The Society for Developmental Biology Innovation Grant was established in 2016 to provide seed funds for those seeking to develop innovative tools and methods with the potential to have a broad impact on the developmental biology community. This work can include devices, software, novel methods, or new reagents, but is not intended to support training or the application of existing technologies. Projects that would not necessarily be supported through other means are given preference. Graduate student, postdoctoral fellow and full SDB members are all eligible. Deadlines are December 1 and May 31. (Please note, if two grants are awarded from the December 1 deadline, then we will not be taking applications for May 31. Therefore, we strongly advise you to submit your application by the December 1 deadline).
University of California, Merced
|Endogenous tissue-specific protein labeling using split fluorescent proteins||The ability to visualize protein localization and dynamics with genetically encoded fluorescent proteins (FPs) is an essential tool for understanding biological processes. Split-FPs are self-complementing fragments that on their own possess little to no fluorescence but together reconstitute a functional fluorescent protein. We propose using a split-Neon Green system (split-NG) to achieve tissue-specific endogenous protein labeling in zebrafish embryos. In our approach, one component of the split-NG complex will be knocked into a gene of interest by CRISPR/Cas9-directed genome editing, while the second component will be expressed under control of a tissue-specific promoter using conventional transgenesis techniques. In this way, fluorescent signal will only be detected in tissues in which both components are co-expressed. Our initial efforts will focus on achieving tissue-specific labeling of broadly expressed cytoskeleton-related genes. Future applications may include forced protein-protein interactions and lineage tracing of cell types best defined by co-expression of two markers.|