Projects focus on differentiation and migration of GnRH cells, olfactory ensheathing cells and olfactory axon outgrowth during development of the craniofacial region. Prenatally, GnRH neurons originate in the nasal region and migrate on olfactory axons into the brain. Current projects examine: 1) lineage of the GnRH cells, 2) intracellular signaling that controls cell movement and corresponding changes in cytoskeletal elements, 3) combinatorial influence of guidance factors involved in migration of these cells into the forebrain; mechanisms common to neuronal migration as well as mechanisms specific to the GnRH system and 4) development of the craniofacial region. Laboratory uses multidisciplinary approaches: cre/lox mice, transgenic lines, imaging, nasal explants, acute slices, videomicroscopy, calcium imaging, electrophysiology, immunocytochemistry, single-cell PCR, CRISPR and subtractive cDNA screening. Candidate should have training in one of the following: Neurobiology, Development or Neuroendocrinology. Technical training in one of the following is required: confocal microscopy, cell culture, calcium imaging, CRISPR, manipulation of gene expression (siRNA, lentivirus, etc.) and/or in situ hybridization on mouse tissue.
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