Gene name - Dynein heavy chain 64C
Cytological map position - 64C1--9
Function - molecular motor
Symbol - Dhc64C
FlyBase ID: FBgn0261797
Genetic map position - 3-
Classification - dynein-(cytoplasmic)-heavy-chain
Cellular location - cytoplasmic
|Recent literature||Arthur, A. L., Yang, S. Z., Abellaneda, A. and Wildonger, J. (2015). Dendrite arborization requires the dynein cofactor NudE. J Cell Sci [Epub ahead of print]. PubMed ID: 25908857
The microtubule-based molecular motor dynein is essential for proper neuronal morphogenesis. Dynein activity is regulated by cofactors whose role(s) in shaping neuronal structure are still being elucidated. Using Drosophila melanogaster, this study revealed that the loss of the dynein cofactor NudE results in abnormal dendrite arborization. The data show that NudE associates with Golgi outposts, which mediate dendrite branching, suggesting NudE normally influences dendrite patterning by regulating Golgi outpost transport. Neurons lacking NudE also have increased microtubule dynamics, reflecting a change in microtubule stability that likely also contributes to abnormal dendrite growth and branching. These defects in dendritogenesis are rescued by elevating Lis1, another dynein cofactor that interacts with NudE as part of a tripartite complex. These data further show that the NudE C-terminus is dispensable for dendrite morphogenesis and likely modulates NudE activity. It is proposed that a key function of NudE is to enhance an interaction between Lis1 and dynein that is critical for motor activity and dendrite architecture.
|Del Castillo, U., Winding, M., Lu, W. and Gelfand, V.I. (2015). Interplay between kinesin-1 and cortical dynein during axonal outgrowth and microtubule organization in Drosophila neurons. Elife 4 [Epub ahead of print]. PubMed ID: 26615019
This study investigates how microtubule motors organize microtubules in Drosophila neurons. It was shown that, during the initial stages of axon outgrowth, microtubules display mixed polarity and minus-end-out microtubules push the tip of the axon, consistent with kinesin-1 driving outgrowth by sliding antiparallel microtubules. At later stages, the microtubule orientation in the axon switches from mixed to uniform polarity with plus-end-out. Dynein knockdown prevents this rearrangement and results in microtubules of mixed orientation in axons and accumulation of microtubule minus-ends at axon tips. Microtubule reorganization requires recruitment of dynein to the actin cortex, as actin depolymerization phenocopies dynein depletion, and direct recruitment of dynein to the membrane bypasses the actin requirement. These results show that cortical dynein slides 'minus-end-out' microtubules from the axon, generating uniform microtubule arrays. The study speculates that differences in microtubule orientation between axons and dendrites could be dictated by differential activity of cortical dynein.
|Jolly, A. L., Luan, C. H., Dusel, B. E., Dunne, S. F., Winding, M., Dixit, V. J., Robins, C., Saluk, J. L., Logan, D. J., Carpenter, A. E., Sharma, M., Dean, D., Cohen, A. R. and Gelfand, V. I. (2016). A genome-wide RNAi screen for microtubule bundle formation and lysosome motility regulation in Drosophila S2 Cells. Cell Rep 14: 611-620. PubMed ID: 26774481
Long-distance intracellular transport of organelles, mRNA, and proteins ("cargo") occurs along the microtubule cytoskeleton by the action of kinesin and dynein motor proteins, but the vast network of factors involved in regulating intracellular cargo transport are still unknown. This study capitalized on the Drosophila melanogaster S2 model cell system to monitor lysosome transport along microtubule bundles, which require enzymatically active kinesin-1 motor protein for their formation. This study used an automated tracking program and a naive Bayesian classifier for the multivariate motility data to analyze 15,683 gene phenotypes and find 98 proteins involved in regulating lysosome motility along microtubules and 48 involved in the formation of microtubule filled processes in S2 cells. Innate immunity genes, ion channels, and signaling proteins were identified having a role in lysosome motility regulation; an unexpected relationship was found between the dynein motor, Rab7a, and lysosome motility regulation.
|Wu, C. H., Zong, Q., Du, A. L., Zhang, W., Yao, H. C., Yu, X. Q. and Wang, Y. F. (2016). Knockdown of Dynamitin in testes significantly decreased male fertility in Drosophila melanogaster. Dev Biol [Epub ahead of print]. PubMed ID: 27742209
Dynamitin (Dmn) is a major component of dynactin, a multiprotein complex playing important roles in a variety of intracellular motile events. Wolbachia bacterial infection has been shown to result in a reduction of Dmn protein. As Wolbachia may modify sperm in male hosts, it is speculated that Dmn may have a function in male fertility. Knockdown of Dmn in testes dramatically decreased male fertility, overexpression of Dmn in Wolbachia-infected males significantly rescued male fertility, indicating an important role of Dmn in inducing male fertility defects following Wolbachia infection. Some scattered immature sperm with late canoe-shaped head distributed in the end of Dmn knockdown testis and only about half mature sperm were observed in the Dmn knockdown testis relative to those in the control. Immunofluorescence staining showed significantly less abundance of tubulin around the nucleus of spermatid and scattered F-actin cones to different extents in the individualization complex (IC) during spermiogenesis in Dmn knockdown testes, which may disrupt the nuclear condensation and sperm individualization. Since dynein-dynactin complex has been shown to mediate transport of many cellular components, including mRNAs and organelles, these results suggest that Dmn may play an important role in Drosophila spermiogenesis by affecting transport of many important cytoplasmic materials.
|Trovisco, V., Belaya, K., Nashchekin, D., Irion, U., Sirinakis, G., Butler, R., Lee, J. J., Gavis, E. R. and St Johnston, D. (2016). bicoid mRNA localises to the Drosophila oocyte anterior by random Dynein-mediated transport and anchoring. Elife 5. PubMed ID: 27791980
bicoid mRNA localises to the Drosophila oocyte anterior from stage 9 of oogenesis onwards to provide a local source for Bicoid protein for embryonic patterning. Live imaging at stage 9 reveals that bicoid mRNA particles undergo rapid Dynein-dependent movements near the oocyte anterior, but with no directional bias. Furthermore, bicoid mRNA localises normally in shot2A2, which abolishes the polarised microtubule organisation. FRAP and photo-conversion experiments demonstrate that the RNA is stably anchored at the anterior, independently of microtubules. Thus, bicoid mRNA is localised by random active transport and anterior anchoring. Super-resolution imaging reveals that bicoid mRNA forms 110-120nm particles with variable RNA content, but constant size. These particles appear to be well-defined structures that package the RNA for transport and anchoring.
|Kreko-Pierce, T. and Eaton, B. A. (2017). The Drosophila LC8 homologue Cut-up specifies the axonal transport of proteasomes. J Cell Sci [Epub ahead of print]. PubMed ID: 28808087
Because of their functional polarity and elongated morphologies, microtubule-based transport of proteins and organelles is critical for normal neuronal function. The proteasome is required throughout the neuron for the highly regulated degradation of a broad set of protein targets whose functions underlie key physiological responses including synaptic plasticity and axonal degeneration. Molecularly, the relationship between proteasome transport and the transport of the targets of proteasomes is unclear. The dynein motor complex is required for the microtubule-based motility of numerous proteins and organelles in neurons. This study demonstrates that microtubule-based transport of proteasomes within the neuron utilizes a distinct dynein light chain compared to synaptic proteins. Live imaging of proteasomes and synaptic vesicle proteins in axons and synapses finds that these cargoes traffic independently and that proteasomes exhibit significantly reduced retrograde transport velocities compared to synaptic vesicle proteins. Genetic and biochemical analyses reveals that the Drosophila homologue of the LC8 dynein light chain Cut-up binds proteasomes and functions specifically during their transport. These data support the model that Cut-up functions to specify the dynein-mediated transport of neuronal proteasomes.
Dynein is a member of the myosin family of molecular motors and is involved in transporting cargo from one part of a cell to another. Equally important, dynein plays a role in cytoskeletal dynamics during mitosis. Axonemal dynein was first discovered in the guise of an ATPase involved in ciliary and flagellar beating. A morphologically similar but distinctly different cytoplasmic form of dynein was subsequently discovered that could translocate organelles toward the minus ends of microtubules. Cytoplasmic dynein is a multisubunit protein (1.2 MDa) consisting of two heavy chains of ~ 500 kDa, each of which fold to form the two heads of the motor, as well as multiple intermediate chains, light intermediate chains, and light chains. Dynein is a retrograde motor, which means that Dynein transports cargo from the terminus of axons back to the cell body from which axons emerge (Karki, 1999 and references).
The protein complex Dynactin, initially identified as a co-purifying set of polypeptides in dynein preparations from rat liver and testis, is also a large multisubunit complex consisting of at least seven distinct polypeptides ranging in size from 22-150 kDa. Dynactin was first demonstrated to be required for dynein-mediated transport in vitro, and is now believed to be required for most, or all, of dynein-mediated cellular activities. A physical interaction between dynein and dynactin has been confirmed by biochemical analyses. Cytoplasmic dynein binds dynactin through a direct interaction between dynein intermediate chains and the p150Glued subunit of dynactin. Dynactin is a platform involved in linking dynein to its cargo or to a substratum. During mitosis, dynactin attaches to a substratum consisting of the kinetochore of each chromosome (Karki, 1999 and references).
Mutations in genes regulating components of the dynein-dynactin complex disrupt axon path finding and synaptogenesis during metamorphosis in the Drosophila central nervous system. In order to learn about the role of the dynein-dynactin complex in the development of the nervous system, the growth of mutant sensory axons during metamorphosis was analyzed. The adult sensory axons grow into the thoracic CNS during the first half of metamorphosis (Shepherd, 1996). The P[Gal4] targeting system can be used to label axons of the femoral chordotonal organ (feCO) in the leg as well as some haltere axons. Using this system, axons are marked on the basis of the expression of specific genes that generate these axons. While most larval sensory neurons degenerate during the first 24 hours of metamorphosis, a segmentally repeated array of 6 neurons per segment persists into the adult stages to become functional adult neurons. These sensory neurons retain their axonal projections in the central nervous system intact and unchanged throughout metamorphosis. The adult sensory neuron axons, originating in the leg or haltere, enter the central nervous system at around 12 hours after puparium formation (APF) (Murphey, 1999). Most of these axons grow along the pathways defined by the persistent larval sensory axons. The ordering of the adult sensory projections is, therefore, established upon the larval pattern of projections (Shepherd, 1996).
Axons entering the first thoracic neuromere were studied because the orientation of three collaterals in this segment allows for ease in scoring. In wild type, the leg chordotonal sensory neuron axons arrive at the CNS in what appears to be a single bundle of growth cones. The bundle grows directly towards the midline without branching and reaches the medial target area by about 24 h APF. At 24 hours APF there is often a detectable defasciculation of the axon bundle in the region that will give rise to the collaterals in the anterior and posterior domains of the first thoracic neuromere. The posterior collaterals (pLAC) are first detected at about 30 hours APF, while the anterior collaterals (sLAC) appear by about 42 hours APF (Murphey, 1999).
In order to better understand the functions of the dynein retrograde motor in nervous system assembly, the pathfinding and arborization of sensory axons during metamorphosis were analyzed in wild-type and mutant backgrounds. As an indicator of dynein function, mutations in two genes were analysed: Glued, coding for the heavy chain of dynactin, and Cytoplasmic dynein light (Cdlc1), coding for a light chain of the dynein protein complex. In Glued1 and Cdlc1 mutants, proprioceptive and tactile axons arrive at the CNS on time but exhibit defects in terminal arborizations that increase in severity up to 48 h after puparium formation. The results show that axon growth occurs on schedule in these mutants but the final process of terminal branching, synaptogenesis, and stabilization of these sensory axons requires the dynein-dynactin complex. Since this complex functions as a retrograde motor, it is suggested that a retrograde signal needs to be transported to the nucleus for the proper termination of some sensory neurons (Murphey, 1999).
Dynein is required in some sensory inputs and not in others. A distinction is made between proprioceptive and tactile axons. Proprioceptors are sensory nerves that convey information about muscle tension, providing input related to the position of an organism in space and to forces experienced by the musculature. Proprioceptive information is provided by chordotonal neurons. Tactile neurons provide information about tactile stimuli received by the organism. A lacZ construct fused to the engrailed promoter was used to examine tactile neurons. en-lacZ-positive sensory axons are located in a posterior bundle in each leg nerve and this bundle extends around the posterior border of each leg neuropil to arborize in a ventral domain (Murphey, 1989b, Phillis, 1996, and Murphey, 1999). en-lacZ expression in tactile axons is activated before the axons reach the CNS and therefore their progress can be followed as the axons enter (Murphey, 1999).
The first pupal sensory axons to be detected in the CNS are a small group of axons that reach and enter the main longitudinal tracts where (between 12 and 24 hours APF) they turn posteriorly in the first thoracic neuromere (T1) or anteriorly in the second and third thoracic neuromeres (T2 and T3). These appear to be the leg 'pioneers' (Bate, 1976). These are unaffected by mutations that affect the function of dynein. The main bundle of tactile axons is first detected at the edge of the ganglion between 12 and 18 hours APF. The bundle extends posteriorly and medially around the neuromere, reaching most of the target areas and exhibiting the mature form by 48 hours. In Gl1 heterozygotes, in approximately 30% of the neuromeres, some of the axons take an aberrant anterior pathway around the neuropil. These axons appear to grow along paths and arborize in domains normally innervated by axons from the posterior, en-lacZ-minus regions of the leg. Thus early axon growth is normal but later crucial events are compromised by the Gl1 mutation and cause defects in arborization in both the tactile and chordotonal sensory axons (Murphey, 1999).
Two groups of sensory axons are unaffected by the Gl1 mutation: the haltere axons and the axons of a group of leg afferents called hair plates. The axons of the haltere nerve enter the CNS, course anteriorly, and give off a group of collaterals near the border of the first and second thoracic neuromeres and project through the connective to the brain. Just as with the feCO chordotonal axons, the haltere axons arrive at the CNS at 6-12 hours APF, grow toward the midline, reach the main longitudinal tract at 18 hours, and grow into the connnective by 24 hours APF (Murphey, 1999).
The differential responses of the various sensory neurons to the Gl1 mutation led to an examination of the three-dimensional structure of the thoracic neuromere in a search for further evidence of the disruption in axon projections. It has been demonstrated that each thoracic neuromere of insects is partitioned into a few discrete domains that are the processing centers for different sensory modalities (Pfluger, 1988 and Murphey, 1989a). Receptors associated with proprioceptive, tactile, and multimodal hairs have been studied (Murphey, 1998a). Proprioceptive hairs occur in clusters, called hair plates, and are situated near joints. The neuron innervating each proprioceptive hair has a large axon and coarse arborization in the CNS in an intermediate neuropil. Tactile receptors have smaller arbors that terminate in a ventral region of the thoracic neuromere. Finally, the multimodal hairs are each innervated by one tactile and four chemosensory neurons. The single tactile neuron has a central arbor that is indistinguishable from those of the tactile hairs; the four chemosensory neurons project to yet a third region of neuropil near the ventral surface of each neuromere. Thus leg sensory axons define two domains in leg neuropil: the intermediate proprioceptive region and the ventral tactile region. For example, the hair plate axons clearly outline the proprioceptive domain in wild type. This domain appears normal in Gl1 mutants as evidenced by the normal projections of the hair plate axons that surround it. Since the hair plate axons exhibit no defects in the mutant background they also define this domain in mutant animals, indicating its relative normality. Despite this apparent normality of the domain structure based on the hair plate projections, the Gl1 mutation disrupts axonal projections from the feCO within the proprioceptive domain (Murphey, 1999 and Reddy, 1997). The tactile receptors project to a separate ventral domain as revealed by the en-lacZ construct (Phillis, 1996). These mutant axons often make pathway choice errors, such as taking an abnormal anterior pathway, but they continue to terminate in the appropriate ventral domain. The results for Cdlc1 mutants are very similar to those obtained with Gl1. It is concluded that four distinct groups of sensory axons give different responses to mutations that affect the function of dynein: the chordotonal and tactile axons are disrupted, while the hair plate and haltere axons are unaffected (Murphey, 1999).
The relatively late onset of defects in the mutant backgrounds, after the axons have reached the CNS, suggests that defects in the dynein-dynactin complex are compromising a retrograde signaling system. This interpretation is that a retrograde signal, received after the sensory growth cones reach the CNS, is crucial to synaptogenesis or synaptic stabilization. Sine the Gl1 gene product disrupts retrograde transport, one possibility is that a retrograde signal must travel from the axon terminal to the nucleus of the sensory neurons in order to have its effect. There appears to be adequate time for retrograde signaling to the cell body, since the round-trip from terminal to soma would take as little as 10 minutes (fast retrograde transport at 1 micrometer/second for a distance of 600 micrometers). The defects seem to arise much more slowly than this, over a time course of approximately 6 hours (Murphey, 1999).
Dynein is also implicated in establishing the orientation of the mitotic spindle early in oogenesis. The orientation of the mitotic spindle has long been known to be important in directing asymmetric cell divisions during embryogenesis. More recently, molecular genetic analyses in C. elegans and Drosophila have identified molecules that couple spindle orientation to the asymmetric localization of cell fate determinants. Members of the myosin family of actin-based molecular motors have been implicated in the asymmetric segregation of factors necessary for cell fate determination in yeast and C. elegans. Less is known about the pathways that establish the initial cellular asymmetry that directs spindle orientation and polarized transport. Given the importance of the microtubule cytoskeleton in cytoplasmic organization and intracellular transport in polarized cell types, microtubules and their associated motor molecules may be part of the primary mechanism that establishes this cytoplasmic asymmetry (McGrail, 1997 and references).
Within the germline cyst a polarity must be established in order to define the pro-oocyte (a single cell selected from 16 germline cells making up the oocytic cyst) as the site of microtubule nucleation in the assembly of the polarized microtubule array. This polarity may be reflected in the asymmetric segregation of the spectrin-rich, multivesicular fusome during the four mitotic divisions (Lin, 1995) that give rise to the oocytic cyst (see alpha Spectrin for more information). The polarity reflects the fixed pattern of cytoplasmic connections among the 16 cystocytes. The asymmetric cystocyte divisions result from the behavior of the fusome and its interaction with spindle poles during mitotic division. It remains unclear how anchorage of the mitotic spindles to the fusome ensures the stereotypical pattern of cyst cell divisions, which creates a polarized cyst leading to oocyte determination, reorganization of the microtubule cytoskeleton, and oocyte differentiation. The identification of mutations in the Drosophila cytoplasmic dynein heavy chain gene Dhc64C (Gepner, 1996) has allowed for an investigation of the role of cytoplasmic dynein in oocyte determination and differentiation. The results provide evidence of a novel role for the cytoplasmic dynein in asymmetric cell division and cellular differentiation (McGrail, 1997).
Mosaic egg chambers whose germline is homozygous for a strong Dhc64C allele fails to produce mature eggs. To understand how oocyte determination is blocked in dynein mutant egg chambers, the polarity of the egg chambers in germline clones was examined. An antibody against a phospho-tyrosine epitope present in ring canals was used to reveal the size and position of the intercellular bridges in wild-type and mutant egg chambers. In the mosaic egg chambers the pattern (e.g., size and location) of the ring canal connections appears abnormal. The most posterior cell in the egg chamber does not have the typical cluster of the 4 largest ring canals. One explanation of this phenotype is based on the disruption of spindle orientation and division pattern, but a cell containing four ring canals may be is mislocalized within the 16-cell cyst. Nonetheless, the mosaic egg chambers always lack an oocyte nucleus, and instead contain 16 polyploid nurse cell nuclei. By contrast, in wild-type egg chambers an oocyte invariably is positioned at the posterior of the egg chamber, and is easily distinguished by the four largest ring canals that reside at the anterior margin of the oocyte. It is also found that Bicaudal-D does not accumulate in a single cell (the oocyte) in dynein mutant cysts. These results suggest that dynein function is required early in germline cyst formation to establish cyst polarity (McGrail, 1997).
Spindle orientation during asymmetric cell division is shown to require cytoplasmic dynein. The orientation and attachment of mitotic spindles to the fusome during cystocyte divisions is thought to ensure the fixed pattern of cell divisions necessary to create the polarized cyst. A striking defect is found in spindle orientation in the dynein mutant cysts. In wild-type mitotic cysts the spindles are arranged in compact clusters with one spindle pole in close contact with a single lobe or branch of the fusome. In contrast, in the mutant cysts the spindles frequently did not appear to contact the fusome, but instead are randomly oriented in the cyst. The defect in spindle orientation suggests that dynein function might be directly involved in the mechanism of spindle orientation. Dynein localization was examined in wild-type mitotic cysts and an enriched, punctate pattern of dynein has been found to be centrally located in a region of the cyst that would correspond to the position of the fusome. No enrichment of dynein on a fusome-like structure is found in interphase cysts. In the 2-cell anaphase cyst and the 4-cell metaphase cyst, each spindle was oriented with one pole in close association to the area of dynein staining. These data suggest that spindle attachment to the fusome is mediated by cytoplasmic dynein. This mechanism would ensure the fixed pattern of cystocyte divisions that lead to a polarized germline cyst (McGrail, 1997).
In addition to its role in the early events of oocyte determination and differentiation, the microtubule cytoskeleton has been implicated in the establishment of axial polarity and the localization of morphogens within the developing oocyte. Female adults doubly heterozygous for the dynein alleles (Dhc64C 6-6 and Dhc64C 6-12) produce late stage egg chambers but are female sterile. The localization of dynein to the posterior of the stage 9 oocyte is disrupted in egg chambers derived from Dhc64C 6-6 ;Dhc64C 6-12 females. Instead, dynein accumulates in a punctate pattern at the anterior margin of the oocyte. The posterior accumulation of a dynein-activating complex, the Glued complex, is also dependent on cytoplasmic dynein. The wild-type Glued complex is mislocalized along with the dysfunctional dynein to the oocyte anterior (McGrail, 1995 and McGrail, 1997).
A role cannot be found for the cytoplasmic dynein motor in the positioning of known morphogens within the oocyte. However, the dynein alleles used in these studies are not null mutations. Therefore a residual level of dynein activity that is insufficient for the proper localization of the Glued complex, but which may be sufficient for directing other mRNA and protein localizations, cannot be excluded. It will be important to identify the cargoes transported by dynein at this later stage of oogenesis. In this regard, the posterior accumulation of a predicted plus-end motor molecule, the kinesin-beta-gal fusion protein, is markedly reduced. Moreover, the fragile nature of the egg shell and the variable defects in the egg shape and chorionic appendages of the mutant eggs suggest another potential role for dynein in the polarized secretion of the egg shell components by the somatic follicle cells (McGrail, 1997).
It has been proposed that spindle positioning ensures the unequal inheritance of the ring canals and associated fusome material at each round of mitosis. This mechanism is believed to account for the production of a branched chain of interconnected cystocytes, in which the two cells with four ring canals and the largest amount of fusome material become pro-oocytes. It is not clear how the selection is made for one of the two cells to continue to differentiate as the oocyte, while the other takes up a nurse cell fate. However, a recent model has addressed this issue and proposes that the future single pro-oocyte is initially determined by the asymmetric segregation of the spectrosome at the first cystoblast division. The identity of the pro-oocyte is maintained at each division by the asymmetric inheritance of the fusome material, which leads to a polarized fusome (Lin, 1995). The polarized fusome may provide the information necessary to subsequently assemble a polarized microtubule array and establish directed transport to the pro-oocyte. Mutations that disrupt the assembly of the fusome and oocyte differentiation, including mutations in the fusome components alpha-spectrin and the adducin-like hu-li tai shao gene product, foster the view that fusome function is critical in oocyte differentiation. This analysis of mitotic germline cysts in the dynein mutant germaria reveals that mitotic spindles fail to acquire the proper orientation with respect to the fusome, and the normal pattern of ring canal connections and cyst polarity is disrupted. These results support the hypothesis that spindle orientation and asymmetric cell division are critical for oocyte determination (McGrail, 1997).
Bases in 5' UTR - 162
The Drosophila cytoplasmic dynein heavy chain is most similar to the predicted rat cytoplasmic dynein heavy chain. Overall the two sequences share 84% similarity and 72% identity at the amino acid level. The homology is distributed across the entire length of the polypeptide. The conserved central domain encompasses a cluster of four phosphate-binding loop (P-loop) motifs. The predicted sequence of the first P-loop (residues 1895-1902) in Drosophila is totally conserved among all dynein sequences. The secondary structure prediction for the Drosophila cytoplasmic dynein heavy chain is similar to those obtained for other dynein sequences and shows that alpha helix, beta sheet, and beta turn conformations are predicted along the entire length of the protein. The prediction is consistent with the predominant globular shape of native dynein complexes as determined by electron microscopy (Li, 1994).
date revised: 10 May 99
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