pebble: Biological Overview | Evolutionary Homologs | Regulation | Developmental Biology | Effects of Mutation | References

Gene name - pebble

Synonyms -

Cytological map position - 66A18-20

Function - signaling

Keywords - cell division, cytoskeleton, required for the formation of the contractile ring and the initiation of cytokinesis

Symbol - pbl

FlyBase ID: FBgn0003041

Genetic map position -

Classification - guanyl-nucleotide exchange factor, Rho GEF

Cellular location - cytoplasmic and nuclear

NCBI link: Entrez Gene
pbl orthologs: Biolitmine

Recent literature
Rosa, A., Vlassaks, E., Pichaud, F. and Baum, B. (2015). Ect2/Pbl acts via Rho and polarity proteins to direct the assembly of an isotropic actomyosin cortex upon mitotic entry. Dev Cell 32: 604-616. PubMed ID: 25703349
Entry into mitosis is accompanied by profound changes in cortical actomyosin organization. This study delineate a pathway downstream of the RhoGEF Pbl/Ect2 that directs this process in Drosophila notum epithelial cells. The data suggest that the release of Pbl/Ect2 from the nucleus at mitotic entry drives Rho-dependent activation of Myosin-II and, in parallel, induces a switch from Arp2/3 (see Actin-related protein 2/3 complex, subunit 1) to Diaphanous-mediated cortical actin nucleation that depends on Cdc42, aPKC, and Par6. At the same time, the mitotic relocalization of these apical protein complexes to more lateral cell surfaces enables Cdc42/aPKC/Par6 to take on a mitosis-specific function-aiding the assembly of a relatively isotropic metaphase cortex. Together, these data reveal how the repolarization and remodeling of the actomyosin cortex are coordinated upon entry into mitosis to provide cells with the isotropic and rigid form they need to undergo faithful chromosome segregation and division in a crowded tissue environment.
Jeong, S., Yang, D. S., Hong, Y. G., Mitchell, S. P., Brown, M. P. and Kolodkin, A. L. (2017). Varicose and cheerio collaborate with pebble to mediate semaphorin-1a reverse signaling in Drosophila. Proc Natl Acad Sci U S A 114(39): E8254-e8263. PubMed ID: 28894005
The transmembrane semaphorin Sema-1a acts as both a ligand and a receptor to regulate axon-axon repulsion during neural development. Pebble (Pbl), a Rho guanine nucleotide exchange factor, mediates Sema-1a reverse signaling through association with the N-terminal region of the Sema-1a intracellular domain (ICD), resulting in cytoskeletal reorganization. This study uncover two additional Sema-1a interacting proteins, varicose (Vari) and cheerio (Cher), each with neuronal functions required for motor axon pathfinding. Vari is a member of the membrane-associated guanylate kinase (MAGUK) family of proteins, members of which can serve as scaffolds to organize signaling complexes. Cher is related to actin filament cross-linking proteins that regulate actin cytoskeleton dynamics. The PDZ domain binding motif found in the most C-terminal region of the Sema-1a ICD is necessary for interaction with Vari, but not Cher, indicative of distinct binding modalities. Pbl/Sema-1a-mediated repulsive guidance is potentiated by both vari and cher Genetic analyses further suggest that scaffolding functions of Vari and Cher play an important role in Pbl-mediated Sema-1a reverse signaling. These results define intracellular components critical for signal transduction from the Sema-1a receptor to the cytoskeleton and provide insight into mechanisms underlying semaphorin-induced localized changes in cytoskeletal organization.
Verma, V. and Maresca, T. J. (2019). Microtubule plus-ends act as physical signaling hubs to activate RhoA during cytokinesis. Elife 8. PubMed ID: 30758285
Microtubules (MTs) are essential for cleavage furrow positioning during cytokinesis, but the mechanisms by which MT-derived signals spatially define regions of cortical contractility are unresolved. In this study cytokinesis regulators visualized in Drosophila melanogaster (Dm) cells were found to localize to and track MT plus-ends during cytokinesis. The RhoA GEF ECT2) did not evidently tip-track, but rather localized rapidly to cortical sites contacted by MT plus-tips, resulting in RhoA activation and enrichment of myosin-regulatory light chain. The MT plus-end localization of centralspindlin was compromised following EB1 depletion, which resulted in a higher incidence of cytokinesis failure. Centralspindlin plus-tip localization depended on the C-terminus and a putative EB1-interaction motif (hxxPTxh) in RacGAP50C. It is proposed that MT plus-end-associated centralspindlin recruits a cortical pool of Dm ECT2 upon physical contact to activate RhoA and to trigger localized contractility.
El-Amine, N., Carim, S. C., Wernike, D. and Hickson, G. R. X. (2019). Rho-dependent control of the Citron kinase, Sticky, drives midbody ring maturation. Mol Biol Cell: mbcE19040194. PubMed ID: 31166845
Rho-dependent proteins control assembly of the cytokinetic contractile ring, yet it remains unclear how those proteins guide ring closure and how they promote subsequent formation of a stable midbody ring. Citron kinase is one important component required for midbody ring formation but its mechanisms of action and relationship with Rho are controversial. This study conducted a structure-function analysis of the Drosophila Citron kinase, Sticky, in Schneider's S2 cells. Two separable and redundant RhoGEF/Pebble-dependent inputs into Sticky recruitment to the nascent midbody ring are defined; each input was shown to be subsequently required for retention at, and for the integrity of, the mature midbody ring. The first input is via an actomyosin-independent interaction between Sticky and Anillin, a key scaffold also required for midbody ring formation. The second input requires the Rho-binding domain of Sticky, whose boundaries were defined in this study. Collectively, these results show how midbody ring biogenesis depends on the coordinated actions of Sticky, Anillin and Rho.
Nakamura, M., Verboon, J. M., Prentiss, C. L. and Parkhurst, S. M. (2020). The kinesin-like protein Pavarotti functions noncanonically to regulate actin dynamics. J Cell Biol 219(9). PubMed ID: 32673395
Pavarotti, the Drosophila MKLP1 orthologue, is a kinesin-like protein that works with Tumbleweed (MgcRacGAP) as the centralspindlin complex. This complex is essential for cytokinesis, where it helps to organize the contractile actomyosin ring at the equator of dividing cells by activating the RhoGEF Pebble. Actomyosin rings also function as the driving force during cell wound repair. Previous work has shown that Tumbleweed and Pebble are required for the cell wound repair process. This study shows that Pavarotti also functions during wound repair and confirm that while Pavarotti, Tumbleweed, and Pebble are all used during this cellular repair, each has a unique localization pattern and knockdown phenotype, demonstrating centralspindlin-independent functions. Surprisingly, it was found that the classically microtubule-associated Pavarotti binds directly to actin in vitro and in vivo and has a noncanonical role directly regulating actin dynamics. Finally, this actin regulation by Pavarotti was shown to not be specific to cellular wound repair but is also used in normal development.
Tavares, L., Gracio, P., Ramos, R., Traquete, R., Relvas, J. B. and Pereira, P. S. (2021). The Pebble/Rho1/Anillin pathway controls polyploidization and axonal wrapping activity in the glial cells of the Drosophila eye. Dev Biol 473: 90-96. PubMed ID: 33581137
During development glial cells are crucially important for the establishment of neuronal networks. Proliferation and migration of glial cells can be modulated by neurons, and in turn glial cells can differentiate to assume key roles such as axonal wrapping and targeting. To explore the roles of actin cytoskeletal rearrangements in glial cells, the function of Rho1 was studied in Drosophila developing visual system. The Pebble (RhoGEF)/Rho1/Anillin pathway is required for glia proliferation and to prevent the formation of large polyploid perineurial glial cells, which can still migrate into the eye disc if generated. Surprisingly, this Rho1 pathway is not necessary to establish the total glial membrane area or for the differentiation of the polyploid perineurial cells. The resulting polyploid wrapping glial cells are able to initiate wrapping of axons in the basal eye disc, however the arrangement and density of glia nuclei and membrane processes in the optic stalk are altered and the ensheathing of the photoreceptor axonal fascicles is reduced.
Montembault, E., Deduyer, I., Claverie, M. C., Bouit, L., Tourasse, N. J., Dupuy, D., McCusker, D. and Royou, A. (2023). Two RhoGEF isoforms with distinct localisation control furrow position during asymmetric cell division. Nat Commun 14(1): 3209. PubMed ID: 37268622
Cytokinesis partitions cellular content between daughter cells. It relies on the formation of an acto-myosin contractile ring, whose constriction induces the ingression of the cleavage furrow between the segregated chromatids. Rho1 GTPase and its RhoGEF (Pbl) are essential for this process. However, how Rho1 is regulated to sustain furrow ingression while maintaining correct furrow position remains poorly defined. This study showed that during asymmetric division of Drosophila neuroblasts, Rho1 is controlled by two Pbl isoforms with distinct localisation. Spindle midzone- and furrow-enriched Pbl-A focuses Rho1 at the furrow to sustain efficient ingression, while Pbl-B pan-plasma membrane localization promotes the broadening of Rho1 activity and the subsequent enrichment of myosin on the entire cortex. This enlarged zone of Rho1 activity is critical to adjust furrow position, thereby preserving correct daughter cell size asymmetry. Our work highlights how the use of isoforms with distinct localisation makes an essential process more robust.

Cytokinesis is the final step in cell division during which the daughter nuclei are invested with their own plasma membranes and cytoplasms. Cytokinesis depends on the formation of a cortical actin- and myosin-based ('actomyosin') contractile ring, which constricts and pinches off the membrane to form the two daughter cells. Pebble is a Rho GEF required for the formation of the contractile ring and the initiation of cytokinesis. Targeted vesicle fusion at the cleavage plane is now known to play an important role in animal cell cytokinesis, with microtubule arrays perhaps helping to direct vesicle transport. Membrane addition may be coordinated with and/or guided by contraction of the actomyosin ring, with these two processes together forming the foundation of cytokinesis in both fungal and animal cells. Several conserved protein families, including the septins, the Cdc15p/PSTPIP proteins, and GTPases of the Rho/Rac/Cdc42 family, act quite broadly in cytokinesis and are likely to direct or coordinate aspects of actomyosin-ring function, membrane addition, or both in various organisms (reviewed by Hales, 1999).

Rho proteins are GTPases that act as molecular switches, cycling between inactive (GDP-bound) and active (GTP-bound) states. Rho is activated by guanine nucleotide exchange factors (GEFs), which enhance the exchange of bound GDP for GTP, and are inactivated by GTPase-activating proteins (GAPs), which increase the intrinsic GTPase activity of Rho proteins. Activation of Rho results in a conformational change of the protein revealing structural domains required for the interaction with downstream target proteins. Thus, the intracellular ratio of the GTP/GDP-bound forms of Rho proteins determines the activation of signal transduction pathways regulating the spatial and temporal reorganization of cytoskeletal architecture (Prokopenko, 1999).

The dynamics of Pbl expression and its distribution during mitosis, as well as structure-function analysis, indicate that it is a key regulatory component of the pathway leading to cytokinesis. In Drosophila, Rho1 has also been identified as a putative effector of DRhoGEF2 (Barrett, 1997; H├Ącker, 1998), indicating that Rho1 is regulated by Pebble and DrhoGEF2, and potentially by other RhoGEFs. pbl interacts genetically with Rho1, but not with Rac1 or Cdc42, and Pbl and Rho1 proteins interact in vivo in yeast. Similar to mutations in pbl, loss of Rho1 or expression of a dominant-negative Rho1 blocks cytokinesis. These results identify Pbl as a RhoGEF specifically required for cytokinesis and linked through Rho1 activity to the reorganization of the actin cytoskeleton at the cleavage furrow (Prokopenko, 1999).

Genetic analysis and biochemical studies carried out in Drosophila have led to the identification of a number of genes required for the assembly or function of the contractile ring (Hale, 1999 and Field, 1999). Structural components of the contractile ring include actin, the regulatory light chain of nonmuscle myosin [encoded by spaghetti-squash (sqh)], septins [Peanut (Pnut), Septin-1, and Septin-2], and actin-binding proteins cofilin [encoded by twinstar (tsr), profilin [encoded by chickadee (chic), and anillin. Mutations in sqh, pnut, tsr, and chic result in a failure of cytokinesis, most likely due to defects in contractile ring organization or function. Yet little is known about the roles of these proteins in contractile ring assembly, stabilization, constriction, and disassembly or about the molecular machinery regulating actin cytoskeleton reorganization during cytokinesis (Prokopenko, 1999).

Pbl redistribution during mitosis parallels the onset of cytokinesis. Double stainings of wild-type embryos undergoing mitotic cycle 14 with anti Pbl antibody and with anti-phospho-histone H3 antibody, which marks chromosomes during mitosis, reveals that Pbl is expressed dynamically during the cell cycle. No Pbl protein is observed during late prophase, metaphase, and early anaphase. Pbl protein is first detected in late anaphase, when it colocalizes with separating daughter chromosomes. The highest levels of Pbl staining were found in telophase nuclei, and this staining persists during interphase of the following mitotic cycle. Since Pbl is never observed at the onset of mitosis, it appears that it is rapidly degraded during each cycle of cell division, presumably after being released from the nucleus upon disassembly of the nuclear envelope in prophase. Further analyses with antibodies against lamin to mark nuclei and alpha-spectrin to stain plasma membranes demonstrates that in late anaphase, there are low levels of Pbl protein at the plasma membrane and in the cytoplasm. Initiation of cytokinesis, as judged by the appearance of the cleavage furrow and reassembly of nuclear laminae, coincides with the accumulation of Pbl at the cleavage furrow. As the cleavage furrow progresses, Pbl accumulates at the equator between dividing cells and in the nuclei of the daughter cells. Pbl translocation to the equatorial region beneath the cleavage furrow parallels the accumulation of anillin and Pnut at the equator where the contractile ring is assembled. In telophase and early interphase cells, the protein is strongly enriched in the nuclei and is found at low levels at the cortex. Hence, during mitosis, subcellular distribution of Pbl undergoes dynamic changes and Pbl is associated with the cleavage furrow during cytokinesis. In addition, the levels of protein are known to cycle during each cell cycle with the highest levels of Pbl in late telophase and interphase (Prokopenko, 1999).

Embryos homozygous for the pbl2 null allele as well as cytological deficiencies removing pbl [Df(3L)pbl-NR] and [Df(3L)25] have greatly reduced levels of staining in cycle 15-16 interphase cells, which is likely to correspond to the remaining maternal contribution. In contrast, pbl5 embryos that carry the V531D missense mutation affecting a conserved valine have protein levels comparable with wild type. It is likely that the reduction in the levels of cytoplasmic, but not nuclear Pbl, is responsible for failure of cytokinesis in mitotic cycles 14-16, because Pbl has never been observed at the cortex or at the cleavage furrow during anaphase and telophase in cycle 14 pbl mutant cells (pbl2 and pbl5 alleles) (Prokopenko, 1999).

The dramatic increase in Pbl levels at the onset of cytokinesis and localization of Pbl to the cleavage furrow suggests that Pbl may play a regulatory role in cytokinesis. As a protein, which presumably regulates the activity of Rho GTPases, Pbl may be required for the initiation of cytokinesis or may couple cytokinesis with the mitotic cycle. To investigate this hypothesis, the effects of overexpression of full-length Pbl and of Pbl protein in which the BRCT domains and the NLS sequence were deleted (DeltaPbl325-853 allele) were compared. The amino-terminal truncation in DeltaPbl325-853 resembles a similar mutation in a number of mammalian RhoGEFs (for review, see Whitehead, 1997), including Ect2 (Miki, 1993), that results in the constitutive activation of their transforming potential. However, the amino-terminal sequences that are lost in different RhoGEFs do not share common domains. Hence, their role in controlling the activity of the full-length protein remains elusive (Prokopenko, 1999).

Ectopic expression of full-length Pbl (with pblEP3415 or the UAS-Pbl3.2line) in stage 8-11 embryos with the paired-GAL4 (prd-GAL4) driver does not result in a detectable phenotype. Although all cells in segments overexpressing Pbl have high levels of exogenous protein in their nuclei and cytoplasm, this does not interfere with cytokinesis, completion of mitosis, or viability. In contrast, expression of DeltaPbl325-853 with the same driver leads to 100% embryonic lethality. Most cells within the embryonic segments expressing DeltaPbl325-853 become multinucleate. This polyploid phenotype is caused by a failure of cytokinesis, because the cleavage furrow is not initiated, and, likewise, the contractile ring is not assembled. The same approach was used to overexpress the amino-terminally truncated form of Ect2, DeltaEct2. As for DeltaPbl325-853, ectopic expression of DeltaEct2 results in the absence of the contractile ring and cleavage furrow, leading to formation of multinucleate cells, and lethality. In contrast, flies expressing full-length Ect2 exhibit no phenotype and are viable (Prokopenko, 1999).

Thus, it appears that full-length Pbl (as well as Ect2) does not behave in vivo as an active protein, because it does not cause a cytokinetic phenotype when expressed in the embryonic ectoderm. This suggests that activation of the exchange activity of Pbl during cytokinesis may require some initiating event. In contrast, amino-terminally truncated Pbl, when overexpressed in the wild-type embryo, has a phenotype similar to lack of pbl. It is proposed that DeltaPbl may behave as an activated protein competing with endogenous Pbl for a rate-limiting target protein (such as Rho GTPase), which results in an inappropriate activation of downstream effectors. Furthermore, the amino-terminal BRCT domains of Pbl, lacking in DeltaPbl, and known to be protein-protein interaction modules, may be required for its regulation by an upstream signal. Such regulation may be essential for the spatial and temporal control of cytokinesis and for the coupling of signaling pathways initiating cytokinesis with other mitotic events (Prokopenko, 1999).

To investigate the possibility that mutations in Rho1 cause defects during cell division, Rho1 embryos were stained with antibodies against lamin and alpha-spectrin. Embryos homozygous for null alleles of Rho1 (Rho172O and Rho172R) show many binucleate cells in the head region. Occasionally, a few polyploid cells are also observed in thoracic or abdominal segments. Although the latter phenotype is observed with low penetrance, binucleate ectodermal cells could never be found in wild-type embryos. The relatively weak phenotype caused by Rho1 mutations (unlike that of pbl) is most likely due to maternally provided Rho1 protein. Thus, it appears that Rho1 is required for cytokinesis, and that decreased levels of Rho1 may account for the observed phenotype in cytokinesis (Prokopenko, 1999).

To further demonstrate the role of Rho1 in cytokinesis, the effects of expression of a dominant-negative form of Rho1, Rho1N19 were analyzed. A dominant-negative form of H-Ras, H-RasN17, cannot interact with downstream target proteins. In addition, H-RasN17 competes with normal H-Ras for binding to rasGEF, which results in formation of inactive H-RasN17-rasGEF complexes and depletion of the pool of the endogenous rasGEF leading to a dominant-negative effect. Therefore, a dominant-negative Rho1 may produce a phenotype similar to or stronger than loss-of-function alleles of Rho1. Ectopic expression of Rho1N19 creates a phenotype that is much worse than loss of zygotic Rho1. Expression of Rho1N19 in ectodermal stripes leads to a complete block of cytokinesis leading to polyploidy of almost every cell within the affected segment. In contrast, expression of wild-type Rho1 or dominant-negative Rac1 (Rac1N17 or Rac1L89) does not affect cytokinesis or completion of mitosis. Interestingly, coexpression of Rho1N19 and Pbl (unlike Pbl alone) results in the mislocalization of Pbl to the cell cortex in interphase and late mitotic cells, further indicating that the two proteins interact during cytokinesis. These results, together with other data, suggest that activation of the Rho1 GTPase by Pbl, its putative exchange factor, is required for the initiation of cytokinesis, because mutations in either protein or overexpression of an inactive protein result in accumulation of multinucleate cells (Prokopenko, 1999).

It is proposed that Pbl, acting at the cleavage furrow, is required for the initiation of cytokinesis. Pbl would function by interacting with and regulating proteins involved in the assembly of the contractile ring. Although the highest levels of Pbl are found in late telophase and young postmitotic nuclei, nuclear Pbl does not seem to play a role in the initiation of cytokinesis. The accumulation of Pbl in telophase nuclei follows the initiation of the cleavage furrow and the beginning of the reassembly of the nuclear envelope and, hence, occurs too late in mitosis to be an instructive signal for cytokinesis. This conclusion is further supported by the observation that amino-terminally truncated Pbl, DeltaPbl, lacking the NLS and localized to the cell cortex and cytoplasm, is able to block cytokinesis. DeltaPbl may compete with the endogenous Pbl at the cleavage furrow for Rho1 or, possibly, other proteins that bind to Pbl (Prokopenko, 1999).

What is the function of nuclear Pbl? The translocation of Pbl to the nuclei may be a regulatory mechanism to turn off the downstream signaling cascade by removing a RhoGEF from the cellular compartment in which the cascade is initiated. Alternatively, there may be two pools of Pbl protein -- a cytoplasmic/cortical pool required for the initiation of cytokinesis and another pool targeted to nuclei in which Pbl plays some other role, unrelated to cytokinesis (Prokopenko, 1999).

Previously, Drosophila Rho1 has been shown to be required for cellularization, gastrulation, dorsal closure, and generation of tissue polarity, but not for cytokinesis. The data suggest that the molecular basis of the genetic interaction between pbl and Rho1 is a physical interaction between Pbl and Rho1 proteins, because the two proteins interact in a yeast two-hybrid assay. Both genetic data and two-hybrid assay results indicate that this interaction is specific for Rho1, but not Rac1 or Cdc42. This interaction presumably results in the activation of the Rho1 GTPase by Pbl and induction of the signaling cascade that initiates the assembly of the contractile ring (Prokopenko, 1999 and references therein).

These results illustrate the complexity of the intracellular signaling pathways involving Rho GTPases and their GEFs. In Drosophila, Rho1 has also been identified as a putative effector of DRhoGEF2, indicating that Rho1 is regulated by at least two RhoGEFs. Given the diversity of developmental roles of Rho1, one would expect that there is a complex hierarchy of molecules regulating Rho1 activity, both spatially and temporally, within a cell. Such differential regulation of Rho1 by upstream signals may be achieved through tissue-specific expression of regulatory molecules (such as GEFs and GAPs) restricted to a particular developmental or cell cycle stage. An additional level of regulation within a cell can be achieved through subcellular compartmentalization of the molecular machinery (such as Rho effectors) required for the activation of distinct pathways mediated by Rho GTPases (Prokopenko, 1999 and references therein).

These observations suggest that Pbl is a key regulatory component of the signaling pathway initiating cytokinesis and therefore, Pbl plays a unique role, different from other cytoskeletal and structural proteins known to be required for cytokinesis. (1) Unlike other proteins required for cytokinesis, the levels of Pbl protein cycle during each round of cell division. (2) Pbl accumulates at the cleavage furrow at the time when cytokinesis is initiated. (3) Pbl is a putative RhoGEF and knowledge about mammalian RhoGEFs clearly implicates them in the regulation of signaling pathways involving Rho GTPases. (4) Mutations within the catalytic DH domain inactivate Pbl function (the pbl5 allele) and abolish Pbl interaction with the Rho1 (PblDeltaDH497-549) allele. In addition, ectopic expression of PblDeltaDH497-549 in the embryo or in the eye imaginal disc blocks cytokinesis and results in the formation of polyploid cells (Prokopenko, 1999).

The data presented here together with earlier observations allow for a proposal of a signaling pathway required for cytokinesis. It is suggested that activation of Rho1 GTPase by Pbl is required for initiation of a molecular pathway leading to assembly of a contractile ring. Such activation is likely to be achieved through direct binding of Pbl and Rho1 at the cleavage furrow. This is consistent with data from other species, in which Rho proteins have been shown to localize to the plasma membrane or cytosol in resting cells, but to translocate to the cleavage furrow and midbody during cytokinesis. Pbl has a similar distribution during mitosis, being initially cortical but accumulating at the cleavage furrow at the onset of cytokinesis. Significantly, coexpression of Pbl and a dominant-negative form of Rho1 results in the accumulation of Pbl at the cell cortex in late mitotic cells, suggesting that the two proteins interact during cytokinesis (Prokopenko, 1999).

Interaction of Rho1 with its effectors is likely to occur at the mouse cleavage furrow as suggested previously for mouse RhoA and p140mDia, a formin-related protein required for cytokinesis (Watanabe. 1997). Both p140mDia and its Drosophila homolog Diaphanous (Castrillon, 1994) localize to the cleavage furrow during cytokinesis. Furthermore, mutations in dia result in the absence of a contractile ring and the failure of cytokinesis. Interestingly, null alleles of dia dominantly suppress the rough eye phenotype of the GMR-Rho1 and GMR-Pbl flies, but dominantly enhance the eye phenotype of GMR-PblDeltaDH flies, suggesting that the three genes are in the same pathway. In addition, ectopic expression of activated Rho1, Rho1V14, but not wild-type Rho1, abolishes cytokinesis. Because mouse p140mDia interacts preferentially with activated RhoA (RhoAV14) but not with wild-type RhoA, it has been suggested that Rho1V14 blocks cytokinesis by titrating out one of its limiting downstream effectors, such as Dia, required for the initiation of cytokinesis. Mouse and Drosophila Dia, as well as other formin-related proteins implicated in cytokinesis (Bni1p in Saccharomyces cerevisiae and cdc12p in Schizosaccharomyces pombe) bind profilin, an actin-binding protein that regulates F-actin polymerization, in vitro and in vivo. In Drosophila, mutations in profilin (encoded by the chic gene) block the assembly of a contractile ring during meiotic cytokinesis (Giansanti. 1998), and in fission yeast, mutations in both genes -- cdc12 (formin) and cdc3(profilin) -- show a synthetic lethal genetic interaction (Prokopenko, 1999 and references therein).

In conclusion, a linear signaling pathway is proposed linking a RhoGEFwith the actin cytoskeleton and, hence, the actomyosin contractile ring. All of the components of this pathway have been implicated in cytokinesis and have been shown to localize to the cleavage furrow during cytokinesis in Drosophila or other organisms. Furthermore, all of the proteins in the pathway interact in vivo or in vitro with their neighboring partners. It is concluded that the activation of Rho1 by Pbl is likely to be an initiating event for the assembly of the contractile ring at the onset of cytokinesis. Identification of upstream components of this pathway will shed light on how cytokinesis is coordinated with the mitotic cycle and on the nature of the signal required for cytokinesis (Prokopenko, 1999).

Adherens junction remodelling during mitotic rounding of pseudostratified epithelial cells

Epithelial cells undergo cortical rounding at the onset of mitosis to enable spindle orientation in the plane of the epithelium. In cuboidal epithelia in culture, the adherens junction protein E-cadherin recruits Pins/LGN/GPSM2 and Mud/NuMA to orient the mitotic spindle. In the pseudostratified columnar epithelial cells of Drosophila, septate junctions recruit Mud/NuMA to orient the spindle, while Pins/LGN/GPSM2 is surprisingly dispensable. This study shows that these pseudostratified epithelial cells downregulate E-cadherin as they round up for mitosis. Preventing cortical rounding by inhibiting Rho-kinase-mediated actomyosin contractility blocks downregulation of E-cadherin during mitosis. Mitotic activation of Rho-kinase depends on the RhoGEF ECT2/Pebble and its binding partners RacGAP1/MgcRacGAP/CYK4/Tum and MKLP1/KIF23/ZEN4/Pav. Cell cycle control of these Rho activators is mediated by the Aurora A and B kinases, which act redundantly during mitotic rounding. Thus, in Drosophila pseudostratified epithelia, disruption of adherens junctions during mitosis necessitates planar spindle orientation by septate junctions to maintain epithelial integrity (Aguilar-Aragon, 2020).

Adherens junctions have long been thought to be continuously essential for maintaining epithelial form and function. The current findings demonstrate transient loss of adherens junctions during division of pseudostratified epithelial cells, an event that involves adherens junction remodelling during the extensive rounding up of cell shape in mitosis. This study has furthermore shown that loss of adherens junctions is a direct consequence of the increased Rho activity and actomyosin contractility that drives mitotic rounding, which is both necessary and sufficient to regulate the level of junctional E-cadherin in the pseudostratified wing imaginal disc epithelium of Drosophila. These findings are consistent with previous observations that adherens junctions can be removed via E-cadherin endocytosis upon planar polarised Rho activation and actomyosin-driven junctional shrinkage generated during cell-cell rearrangements in the Drosophila embryo and recent optogenetic experiments in human cells. However, the global loss of E-cadherin observed during mitosis of pseudostratified epithelial cells is unprecedented and may be uniquely required by the rapid transformation of these cells from their highly columnar shape to a rounded sphere at mitosis, which involves a rapid increase in apical area and junctional length. This change in cell chape, driven by global actomyosin contractility during mitotic rounding, may both spread out junctions and disrupt cadherin-cadherin contacts between neighbouring cells to favour endocytosis (Aguilar-Aragon, 2020).

Notably, loss of E-cadherin does not seem to occur during mitosis in cuboidal epithelial cells, which undergo a much milder cell shape change during mitotic rounding, such as the Drosophila follicle cell epithelium or in many human cultured epithelial cell lines. Indeed, E-cadherin was reported to play essential roles in planar spindle orientation in cultured human cells. Thus, the pseudostratified epithelia of Drosophila face a unique challenge of orienting the mitotic spindle in the plane of the epithelium without the use of adherens junctions as a cue, which may explain why these cells instead rely on septate junctions, while planar spindle orientation can occur normally in the cuboidal follicle cell epithelium before septate junctions form. In the absence of septate junctions, spindle-orienting factors such as Pins, Mud, Dlg and Scrib localise to lateral membranes, overlapping with E-cadherin, which can directly interact with Scrib in both Drosophila and human cells (Aguilar-Aragon, 2020).

The findings of this study also shed light on the molecular mechanisms linking the cell cycle with control of Rho activation during mitotic rounding. Downstream of the master mitotic kinase Cdk1, a key role for both Aurora A and B kinases (acting redundantly) was identified in initiating mitosis and maintaining cortical rounding, with Aurora B then acting alone to drive furrow formation during cytokinesis. Aurora kinases are known to activate the key cell cycle kinase Plk1/Polo, which can then activate RacGAP1/MgcRacGAP/CYK4/Tum and ECT2/Pbl, possibly via the kinesin-like protein MKLP1/KIF23/ZEN4/Pav. One report suggested that Aurora B also acts to directly phosphorylate RacGAP1/MgcRacGAP/CYK4/Tum on S387, but this study found that a CRISPR-knockin mutation of this site (tumS387A) is homozygous viable and fertile in Drosophila. In addition, Aurora B can also directly phosphorylate MKLP1/KIF23/ZEN4/Pav to oligomerise and activate RacGAP1/MgcRacGAP/CYK4/Tum and ECT2/Pbl during cytokinesis, via a mechanism involving plasma membrane association of clustered C1 domains from the RacGAP1/MgcRacGAP/CYK4/Tum protein. Accordingly, it was found that a CRISPR-knockin mutation of this site (pavS734A/S735A) is homozygous lethal in Drosophila, suggesting that Aurora A/B could act via this complex during mitotic rounding. Indeed, MKLP1/KIF23/ZEN4/Pav undergoes cell cycle-dependent re-localisation from the nucleus (in interphase) to the cytoplasm (in mitosis), including a clear localisation to the entire plasma membrane during mitosis and then to the cleavage furrow during cytokinesis. The results show that mitotic activation of the MKLP1/KIF23/ZEN4/Pav binding partner RacGAP1/MgcRacGAP/CYK4/Tum also involves translocation from the nucleus to the cytoplasm, similar to ECT2/Pbl, where all three proteins are then available to bind the entire plasma membrane and generate global Rho activity to drive cortical contractility and loss of adherens junctions during mitotic rounding (Aguilar-Aragon, 2020).

The complex of MKLP1/KIF23/ZEN4/Pav and RacGAP1/MgcRacGAP/CYK4/Tum is often referred to as the 'centralspindlin' complex, due to its association with the spindle midzone at anaphase. However, the use of this term was avoided because association with the spindle midzone is not required for the function of this complex in activating ECT2/Pbl and Rho at the plasma membrane during cytokinesis. Furthermore, the results show that the same complex also functions prior to anaphase or cytokinesis in activating ECT2/Pbl and Rho for cortical rounding and downregulation of adherens junctions from the very onset of mitosis (Aguilar-Aragon, 2020).

Importantly, the function of the three cell cycle-regulated Rho activators discussed in this study - MKLP1/KIF23/ZEN4/Pav, RacGAP1/MgcRacGAP/CYK4/Tum and ECT2/Pbl - on the regulation of adherens junctions appears to be dose-dependent and cell shape change-dependent. The sudden re-localisation of these proteins to the cytoplasm at mitosis drives a global increase in Rho activation, cell shape change and loss of junctions. In contrast, the relatively low level of these proteins in the cytoplasm during interphase appears to contribute to the normal maintenance of Rho activity at the junctional actomyosin ring to maintain adherens junctions-at least in mammalian cells. In Drosophila, an interphase role of ECT2/Pbl in contributing to Rho activation and maintenance of adherens junctions is also plausible and should be most easily distinguished from the mitotic role in non-dividing epithelial cells. However, in the ovarian follicle cell epithelium, silencing of ECT2/Pbl by RNAi affected cytokinesis (leading to larger cells) but did not affect overall epithelial architecture after cells arrest their proliferation, suggesting that any interphase function may be obscured by redundancy with other RhoGEFs in this tissue. Note also that, in this cuboidal epithelium, mitotic rounding itself is more subtle and ECT2/Pbl is dispensable for planar spindle orientation and epithelial integrity (Aguilar-Aragon, 2020).

Finally, it is noted that ECT2/Pbl was initially reported to be capable of acting as GEF for another GTPase, Cdc42, in addition to Rho 38. This activity suggested a possible role in regulating the apical Cdc42-Par6-aPKC complex in epithelial polarity and in mitosis. In Drosophila, ECT2/Pbl was found to drive transient apical spreading of the Cdc42-Par6-aPKC complex during mitotic rounding of pupal notum epithelial cells. This study found evidence for a similar function of ECT2/Pbl in activating Cdc42 during interphase in the post-mitotic ovarian follicle cell epithelium, although the loss-of-function phenotype of ECT2/Pbl is obscured due to redundancy with other Cdc42 GEFs such as beta-PIX. Loss of both ECT2/Pbl and beta-PIX reduced the level of the Cdc42-Par6-aPKC complex localising to the apical domain of post-mitotic follicle cells and also reduced apical ZO-1 localisation in human Caco2 intestinal epithelial cells in culture. In contrast, the gain-of-function phenotype caused by ECT2/Pbl overexpression is a neoplastic tumour-like phenotype and clearly involves ectopic spreading of the apical Cdc42-Par6-aPKC complex, in addition to persistent cell rounding in both cuboidal follicle cells and pseudostratified wing epithelial cells (Aguilar-Aragon, 2020).

In conclusion, the findings provide new insights into the cell biology of mitotic rounding, identifying remodelling of adherens junctions as a key event in pseudostratified epithelia, where rounding is extensive, but not cuboidal epithelia, where rounding is more subtle. These results are consistent with the hypothesis that Rho activation and actomyosin contractility can stabilise adherens junctions in the absence of mechanical strain, but that Rho activation can induce E-cadherin endocytosis above a critical strain threshold, be it either junctional shrinkage or expansion, both of which may alter the geometry of the junctional actomyosin ring and disrupt cadherin-cadherin contacts between neighbouring cells to favour endocytic internalisation of E-cadherin. The results also clarify the molecular mechanisms linking cell cycle control machinery, particularly the Aurora A and B kinases, with Rho activation and mitotic rounding. Lastly, this work may have direct relevance to certain human epithelial cancers, such as lung cancer or glioma, where overexpression of ECT2/Pbl has been reported to correlate for poor prognosis, and where the findings suggest it could drive not only disruption of epithelial polarity via activation of Cdc42 or Rac but also loss of adherens junctions via sustained Rho activation to promote tumour progression (Aguilar-Aragon, 2020).


Amino Acids - 853

Structural Domains

pbl encodes a novel RhoGEF related to the mouse Ect2 oncoprotein. The lengths of Pbl and Ect2 are 853 and 738 amino acids, respectively. Overall, the two proteins are 40% identical and 61% similar. Common functional domains of Pbl and Ect2 include the Dbl homology (DH) and pleckstrin homology (PH) domains (in Pbl, amino acids 390-574 and 600-718, respectively) that are found in tandem in all RhoGEFs. Mammalian members of the RhoGEF family are considered to be potential oncogenes because their truncated forms often induce transformed foci when overexpressed in cultured fibroblasts. The DH domain catalyzes GDP/GTP exchange through direct binding to its effector GTPase (Hart, 1994; Zheng, 1995; Liu, 1998). The PH domain (Shaw, 1996), always located carboxy-terminal to the DH domain in RhoGEFs, is thought to be required for membrane or cytoskeletal targeting of the protein and in enhancement of the catalytic activity of the adjacent DH domain (Whitehead, 1995; Zheng, 1996; Liu, 1998; Sterpetti, 1999). At the amino terminus, Pbl and Ect2 share two BRCT (BRCA1 carboxyl terminus) domains (in Pbl, amino acids 113-198 and 208-291) initially identified in the familial breast and ovarian cancer susceptibility gene BRCA1 (Callebaut, 1997). BRCT domains are found in proteins involved in DNA metabolism and cell cycle checkpoint control and are thought to be protein-protein interaction modules. In addition, Pbl contains a putative nuclear localization signal (NLS) (amino acids 306-322) and a PEST sequence (amino acids 371-383), which often serves as a proteolytic signal to target proteins for rapid degradation (Prokopenko, 1999 and references therein).

pebble: Evolutionary Homologs | Regulation | Developmental Biology | Effects of Mutation | References

date revised: 5 December 2023

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