The Interactive Fly

Zygotically transcribed genes

Heat shock factor and heat shock proteins

Heat shock factor

  • Interplay between RNA interference and heat shock response systems in Drosophila melanogaster
  • Linear ubiquitination by LUBEL has a role in Drosophila heat stress response
  • Expression of thermal tolerance genes in two Drosophila species with different acclimation capacities
  • Expression of hsromega-RNAi transgene prior to heat shock specifically compromises accumulation of heat shock-induced Hsp70 in Drosophila melanogaster
  • The molecular chaperone Hsp70 from the thermotolerant Diptera species differs from the Drosophila paralog in its thermostability and higher refolding capacity at extreme temperatures
  • Small heat shock proteins determine synapse number and neuronal activity during development
  • Small heat shock protein Hsp67Bc plays a significant role in Drosophila melanogaster cold-stress tolerance
  • The histone replacement gene His4r is involved in heat stress induced chromatin rearrangement
    Proteins and RNAs that mediate the magnitude and efficiency of the heat shock response
    Heat shock proteins

    Interplay between RNA interference and heat shock response systems in Drosophila melanogaster

    The genome expression pattern is strongly modified during the heat shock response (HSR) to form an adaptive state. This may be partly achieved by modulating microRNA levels that control the expression of a great number of genes that are embedded within the gene circuitry. This study investigated the cross-talk between two highly conserved and universal house-keeping systems, the HSR and microRNA machinery, in Drosophila melanogaster. Pronounced interstrain differences in the microRNA levels are alleviated after heat shock (HS) to form a uniform microRNA pattern. However, individual strains exhibit different patterns of microRNA expression during the course of recovery. Importantly, HS-regulated microRNAs may target functionally similar HS-responsive genes involved in the HSR. Despite the observed general downregulation of primary microRNA precursor expression as well as core microRNA pathway genes after HS, the levels of many mature microRNAs are upregulated. This indicates that the regulation of miRNA expression after HS occurs at transcriptional and post-transcriptional levels. Deletion of all hsp70 genes had no significant effect on microRNA biogenesis but might influence the dynamics of microRNA expression during the HSR (Funikov, 2016).

    Linear ubiquitination by LUBEL has a role in Drosophila heat stress response

    The HOIP ubiquitin E3 ligase generates linear ubiquitin chains by forming a complex with HOIL-1L and SHARPIN in mammals. This study provides the first evidence of linear ubiquitination induced by a HOIP orthologue in Drosophila. This study identified Drosophila CG11321, which was named Linear Ubiquitin E3 ligase (LUBEL), and it was found to catalyze linear ubiquitination in vitro. Endogenous linear ubiquitin chain-derived peptides were detected by mass spectrometry in Drosophila Schneider 2 cells and adult flies. Furthermore, using CRISPR/Cas9 technology, linear ubiquitination-defective flies were established by mutating residues essential for the catalytic activity of LUBEL Linear ubiquitination signals accumulate upon heat shock in flies. Interestingly, flies with LUBEL mutations display reduced survival and climbing defects upon heat shock, which is also observed upon specific LUBEL depletion in muscle. Thus, LUBEL is involved in the heat response by controlling linear ubiquitination in flies (Asaoka, 2016).

    Expression of thermal tolerance genes in two Drosophila species with different acclimation capacities

    Heat tolerance increases at higher acclimation temperatures in D. melanogaster, but not in D. subobscura. The two species represent separate lineages of the subgenus Sophophora of Drosophila with contrasting tropical African and temperate Palearctic evolutionary histories. D. melanogaster has five copies of the inducible hsp70 gene distributed in two clusters, named A (with two copies) and B (three copies), while D. subobscura has only two copies arranged similarly to cluster A of D. melanogaster. The hsp70s of the two species also differ in their cis-regulatory regions, with D. melanogaster exhibiting features of a faster and more productive promoter. It was predicted that the interspecific variation in acclimation capacity of heat tolerance is explained by evolved variation in expression of the major group of heat shock proteins. To test this prediction, basal levels of gene expression were compared at different developmental temperatures within each of the two species. Furthermore, the heat hardening dynamics were explored by measuring the induction of gene expression during a ramping assay. The prediction of a stronger heat shock protein response in D. melanogaster as compared to D. subobscura was confirmed for both long-term acclimation and short-term hardening. For D. melanogaster the upregulation with temperature ramping ranged from less than two fold (hsp26) to 2500 fold (hsp70A) increase. In all cases induction in D. melanogaster exceeded that of D. subobscura homologs. These differences correlate with structural differences in the regulatory regions of hsp70, and might explain differences in acclimation capacity among species. Finally, in D. melanogaster an indication was found of an inverse relationship between basal and induced levels of hsp70A and hsp83 expression, suggesting a divergent role for thermal adaptation of these genes at benign and stressful temperatures, respectively (Sorensen, 2019).

    Expression of hsromega-RNAi transgene prior to heat shock specifically compromises accumulation of heat shock-induced Hsp70 in Drosophila melanogaster

    A delayed organismic lethality has been reported in Drosophila following heat shock when developmentally active and stress-inducible noncoding hsrω-n transcripts were down-regulated during heat shock through hs-GAL4-driven expression of the hsrω-RNAi transgene, despite the characteristic elevation of all heat shock proteins (Hsp), including Hsp70. This study shows that hsrω-RNAi transgene expression prior to heat shock singularly prevents accumulation of Hsp70 in all larval tissues without affecting transcriptional induction of hsp70 genes and stability of their transcripts. Absence of the stress-induced Hsp70 accumulation was not due to higher levels of Hsc70 in hsrω-RNAi transgene-expressing tissues. Inhibition of proteasomal activity during heat shock restored high levels of the induced Hsp70, suggesting very rapid degradation of the Hsp70 even during the stress when hsrω-RNAi transgene was expressed ahead of heat shock. Unexpectedly, while complete absence of hsrω transcripts in hsrω66 homozygotes (hsrω-null) did not prevent high accumulation of heat shock-induced Hsp70, hsrω-RNAi transgene expression in hsrω-null background blocked Hsp70 accumulation. Nonspecific RNAi transgene expression did not affect Hsp70 induction. These observations reveal that, under certain conditions, the stress-induced Hsp70 can be selectively and rapidly targeted for proteasomal degradation even during heat shock. In the present case, the selective degradation of Hsp70 does not appear to be due to down-regulation of the hsrω-n transcripts per se; rather, this may be an indirect effect of the expression of hsrω-RNAi transgene whose RNA products may titrate away some RNA-binding proteins which may also be essential for stability of the induced Hsp70 (Singh, 2016).

    Heat shock response is one of the most conserved cellular cascades that effectively protect cells and the organism from adverse environmental conditions like physiologically high temperature, oxidative stress, cytotoxins, etc.. While the classical hallmark of the cell stress response has been the rapid induction of different families of heat shock or stress proteins, multiple long noncoding RNAs (lncRNAs) are now also known to be involved in the cell stress response. The 93D/hsrω gene of Drosophila, with its multiple transcripts, is the earliest known lncRNA gene having significant roles in development and in cell stress response. The hsrω gene comprises a proximal region (∼2.6 kb), with two exons, E1 (~475 bp) and E2 (~750 bp), an intron (~700 bp), and a distal >5-kb region carrying short tandem repeats of 280 bp, unique to the locus. Until recently, it was believed to produce two primary transcripts, viz., hsrω-n1 (hsrω-RB) and hsrω-pre-c (hsrω-RC) from which the intron is spliced out to produce the hsrω-n2 (hsrω-RG) and hsrω-c (hsrω-RA) transcripts, respectively. Out of these four hsrω transcripts (two primary and two processed), the hsrω-c is cytoplasmic, while the other three are nuclear. Recent annotation at the Flybase indicates that the hsrω gene is longer than previously believed and produces additional transcripts. Little is known about the newly annotated hsrω transcripts although other studies confirm the presence of these new transcripts (hsrω-RD and hsrω-RF) and that the nearly 21 kb hsrω-RF transcript is heat shock inducible. A very small 23-bp long translatable ORF is present in the hsrω-c of Drosophila melanogaster, although its translation product has not yet been identified (Singh, 2016).

    The nucleus-limited hsrω-n transcripts (hsrω-RB and RG on Flybase) interact with several RNA processing proteins and organize the nucleoplasmic omega speckles. The omega speckles function as storage sites for several RNA processing proteins, which are dynamically released from or sequestered by the hsrω-n transcripts according to cellular needs. The hsrω-null individuals are poorly viable and are thermosensitive. A recent study showed that conditional down- or up-regulation of the hsrω nuclear transcripts through hs-GAL4-driven activation of UAS-hsrω-RNAi transgene or of EP alleles of hsrω, respectively, during heat shock results in delayed organismic lethality in spite of the characteristic elevation in cellular levels of the different HSPs, including the Hsp70. The delayed organismic lethality in these genotypes during recovery was correlated with the absence of omega speckles and a slow and incomplete restoration of the hnRNPs on developmentally active gene loci during recovery after heat shock. It is known that global activation of UAS-hsrω-RNAi transgene by Act-GAL4 driver also disrupts the omega speckles. Therefore, it was of interest to see how the disruption of omega speckles in unstressed cells through down- or up-regulation of hsrω transcripts affects their stress response. Accordingly, the present study has examined heat shock response in tissues where hsrω transcripts were down- or up-regulated sometime before the cells were exposed to heat shock. It was found that expression of UAS-hsrω-RNAi transgene in unstressed cells severely affected the cellular levels of Hsp70 during heat shock as well as during subsequent recovery. Interestingly, expression of the UAS-hsrω-RNAi transgene did not affect heat shock-induced transcription, transport, and stability of the hsp70 messenger RNAs (mRNAs) but enhanced rapid degradation of the synthesized Hsp70 through proteasomal pathway even when the cells were under stress (Singh, 2016).

    The noncoding hsrω gene of D. melanogaster is developmentally expressed in almost all cell types and is one of the most highly induced genes following heat shock. The nuclear transcripts of hsrω gene, hsrω-n1, and hsrω-n2 are essential for organization of the omega speckles which are believed to regulate the availability of various hnRNPs and certain other RNA-binding proteins. In an earlier study, it was seen that when the UAS-hsrω-RNAi transgene or an EP allele of hsrω (EP93D or EP3037) was activated during heat shock using the hs-GAL4 driver, the major heat shock genes and stress proteins like Hsp70 and Hsp83 were characteristically induced, yet all the individuals exhibited delayed death during recovery. This delayed death was correlated with the delayed restoration of RNA Pol II, HP1, and hnRNPs to the developmentally active gene loci when UAS-hsrω-RNAi transgene or an EP allele of hsrω was expressed during heat shock (Lakhotia, 2012; Singh, 2016 and references therein).

    The present study used Act-GAL4 or Sgs-GAL4 or GMR-GAL4 drivers, instead of the hs-GAL4 driver, to alter the levels of hsrω transcripts much before the tissues experienced heat shock. In these cases also, as in earlier study (Lakhotia, 2012), a delayed restoration of hnRNPs, etc. to their normal chromosomal sites was seen during recovery from heat shock, which may be the major factor for the observed delayed death. The most striking effect of expression of the hsrω-RNAi transgene, but not of the EP3037 allele, prior to heat shock is the near complete absence of Hsp70 in heat-shocked cells. The former would down-regulate levels of hsrω-n transcripts while the latter would result in their elevated levels. The present results show that cells with down-regulated levels of hsrω transcripts fail to elevate the levels of Hsp70 when heat shocked, although Hsp83 showed the expected increase (Singh, 2016).

    Normally, the stress-inducible Hsp70 shows the most robust increase in stressed cells and continues to remain so for a few hours even after the stress condition is withdrawn. Only in certain WT tissues, like the MT and midgut polytene cells, this protein's inducibility by heat shock follows a different pattern. However, the Hsp70 accumulation after heat shock failed to follow the pattern characteristic of even these tissues when the UAS-hsrω-RNAi transgene was expressed prior to heat shock (Singh, 2016).

    The continued puffing at the Hsp70-encoding 87A and 87C sites even after 4-h recovery from heat shock in Act-GAL4>hsrω-RNAi-expressing salivary gland (SG) also appears to be a consequence of the greatly reduced levels of Hsp70 in these cells since an optimal level of Hsp70 is required to autoregulate the stress-induced heat shock genes, including the hsp70 gene copies. Several earlier studies showed that a subnormal induction of the 93D puff harboring the hsrω gene during heat shock affects puffing at the 87A and 87C sites in a condition-specific manner (reviewed in Lakhotia, 1989; Lakhotia, 2011). In the present study also, it was noted that the 87A puff was generally smaller than the 87C puff in heat-shocked SGs that were already expressing the UAS-hsrω-RNAi transgene. However, the unequal puffing of the 87A and 87C puffs is unlikely to be responsible for the drastically reduced levels of Hsp70 in these cells. Observations on in situ localization of active RNA Pol II and hsp70 transcripts on the heat shock-induced 87A and 87C puffs and elsewhere show that heat shock-induced active transcription of the hsp70 genes and transport of hsp70 mRNAs from nucleus to cytoplasm are not affected by expression of UAS-hsrω-RNAi under the Act-GAL4 driver. Since levels of heat shock-induced hsp70 transcripts, as detected by RT-PCR, remained comparable and immunostaining for a P-body-specific GW182 protein did not reveal any significant difference in the distribution of P-bodies in different genotypes, it is unlikely that the hsp70 transcripts are prematurely degraded when the UAS-hsrω-RNAi transgene is expressed prior to heat shock under Act-GAL4 or Sgs3-GAL4 or GMR-GAL4 drivers. This is also supported by the observation that even 10 min after heat shock, these glands failed to show the characteristic high induction of Hsp70 (Singh, 2016).

    Specific function of the 1.2 kb cytoplasmic hsrω-c transcript during development or in stress response is not known; however, its small translatable ORF has been suggested to have some role in translational activities in cells. Although earlier studies indicated that expression of the hsrω 280-bp repeat sequence present in the hsrω-RNAi transgene does not affect the levels of hsrω-c transcripts, it remains possible that the levels of hsrω-pre-c and, therefore, of the hsrω-c transcripts too may be affected due to the process of RNAi amplification, which in turn may affect translatability of the hsp70 transcripts. Therefore, this study also examined heat shock-induced accumulation of Hsp70 in cells co-expressing UAS-hsrω-RNAi and UAS-hsrω-pre-c transgenes. Since in this case, also Hsp70 levels remained as low as in only hsrω-RNAi transgene-expressing cells, it is believed that the potentially reduced levels of hsrω-pre-c and hsrω-c transcripts are unlikely to be a reason for the observed low levels of Hsp70 in the heat-shocked hsrω-RNAi-expressing cells. These studies also show that global expression of hsrω-RNAi transgene does not affect the basal levels of different Hsc70 proteins in unstressed cells. The 7.10.3 antibody detects cognate as well as the induced members of the Hsp70 family in Drosophila. In agreement with results obtained with the 7Fb ab, which detects only the stress-inducible Hsp70, the 7.10.3 ab signal in heat-shocked samples from hsrω-RNAi transgene-expressing larvae was much less intense than in WT. Thus, autoregulation of Hsp70 induction by enhanced levels of Hsc70s does not appear to be a reason for noninduction of Hsp70 in hsrω-RNAi background (Singh, 2016).

    The current observations suggest that the absence or very little presence of Hsp70 in cells that have been expressing the UAS-hsrω-RNAi transgene before they were exposed to the thermal stress is due to specific degradation of Hsp70 by the proteasomal machinery since, when the proteasome inhibitor was present during heat shock, the Hsp70 accumulated in UAS-hsrω-RNAi transgene-expressing cells to the same extent as in other genotypes. Earlier studies have indeed shown that expression of UAS-hsrω-RNAi transgene enhances proteasomal activity. It is intriguing that the improved proteasomal activity specifically removes the stress-induced Hsp70 but not any other HSP. It is known that Hsp70 follows a different pathway for degradation than other HSPs and, therefore, might be singularly targeted in hsrω-RNAi transgene-expressing cells during heat shock and recovery. It is intriguing that Hsp70 does not accumulate in stressed cells only when the UAS-hsrω-RNAi transgene is activated sometime before the heat shock (present results), but its accumulation remains unaffected when this transgene is activated along with heat shock (Singh, 2016).

    The stress-induced Hsp70 is known to be rapidly removed by proteasomal degradation in WT cells recovering from the stress following its ubiquitination by the carboxy terminus of Hsp70-binding protein (CHIP). This protein plays a dual role in the heat shock response by ubiquitination of misfolded proteins and presenting them to Hsp70 during stress, and when misfolded proteins are no longer available during recovery, CHIP ubiquitinates Hsp70 for proteasomal degradation. It remains to be seen if CHIP is involved in the unusually rapid and premature degradation of Hsp70 or some other factors are at play in cells that express the UAS-hsrω-RNAi transgene prior to heat shock (Singh, 2016).

    The findings that hsrω-null (hsrω66 homozygous) condition does not affect the heat shock-induced massive accumulation of Hsp70 but expression of UAS-hsrω-RNAi transgene in hsrω-null background affects Hsp70 levels after heat shock were completely unexpected. The first condition may suggest that a complete absence of hsrω gene since fertilization, as in hsrω66 homozygotes, but not subthreshold levels of hsrω transcripts, as in cells expressing the hsrω-RNAi transgene prior to heat shock, may affect the Hsp70 accumulation. However, absence of heat shock-induced Hsp70 protein when the hsrω-RNAi transgene is expressed in hsrω-null background is very perplexing since these cells do not have any target for the RNAi, as none of the hsrω transcripts is present. This raises the intriguing possibility that the observed effect may not be a consequence of down-regulation of the hsrω transcripts per se but may be a consequence of this transgene's expression itself. The hsrω-RNAi transgene construct includes a monomer of the 280-bp tandem repeats at the hsrω gene, taken from the pDRM30 clone together with ~80 bp of flanking vector sequence. The SympUAS vector would cause transcription of both strands of the 280 bp plus the flanking vector sequence when GAL4 is available. The 280-bp repeat sequence of the hsrω-n transcripts binds with several proteins. It remains possible that binding of some protein/s to the sense and/or antisense strands over-produced by this transgene construct may lead to functional depletion of that/those protein/s, which in turn may affect stability of the heat shock-induced Hsp70. It is known that excessive production of some RNAs or triplet repeat expansion in certain RNAs sequesters specific proteins resulting in dysregulation of their activities and thus proteinopathies. The current results with three other RNAi transgenic lines (Act-GAL4>Egfr-RNAi or Act-GAL4>Hsp60C-RNAi or Act-GAL4>Hsp60D-RNAi), each of which includes a long (>400 bp) RNAi construct, show that the absence of inducible Hsp70 in hsrω-RNAi transgene-expressing background is specific for expression of the hsrω-RNAi transgene. The protein-binding activities of each of the two strands produced by the SympUAS-hsrω-RNAi transgene construct when activated by GAL4 need to be examined. The dsRNA-dependent protein kinase, protein kinase R (PKR), has been implicated in determining the stability of hsp70 mRNA in mammalian cells such that PKR-null cells do not show significant accumulation of Hsp70 when exposed to cell stress. Although the results suggest that the heat-shocked-induced hsp70 transcripts are not destabilized by hsrω-RNAi transgene expression, it would be interesting to examine if the PKR homologues in Drosophila affect Hsp70 protein's stability (Singh, 2016).

    The present observations appear to be the first to show selective instability of heat shock-induced Hsp70 so that it gets targeted to proteasomal degradation as soon as synthesized while the other induced heat shock proteins remain stable. This adds a new dimension to the complexity of regulation of the cell stress response. This study also brings out additional issues that may need to be considered when applying experimental RNAi approach. Normally, one expects the sense strand of the RNAi construct to get degraded. However, if it does not happen and if the RNA sequence can bind with some proteins, unexpected consequences may also follow (Singh, 2016).

    The molecular chaperone Hsp70 from the thermotolerant Diptera species differs from the Drosophila paralog in its thermostability and higher refolding capacity at extreme temperatures

    Previous work has demonstrated that species of the Stratiomyidae family exhibit higher tolerance to thermal stress in comparison with that of many representatives of Diptera, including Drosophila species. It was hypothesized that species of this group inherited the specific structures of their chaperones from an ancestor of the Stratiomyidae family, and this enabled the descendants to colonize various extreme habitats. To explore this possibility, copies of the Hsp70 genes from Stratiomys singularior, a typical eurythermal species, and Drosophila melanogaster, were cloned and expressed in Escherichia coli for comparison. To investigate the thermal sensitivity of the chaperone function of the inducible 70-kDa heat shock proteins from these species, an in vitro refolding luciferase assay was used. Under conditions of elevated temperature, S. singularior Hsp70 exhibited higher reactivation activity in comparison with D. melanogaster Hsp70 and even human Hsp70. Similarly, S. singularior Hsp70 was significantly more thermostable and showed in vitro refolding activity after preheatment at higher temperatures than D. melanogaster paralog. Thermally induced unfolding experiments using differential scanning calorimetry indicated that Hsp70 from both Diptera species is formed by two domains with different thermal stabilities and that the ATP-binding domain of S. singularior is stable at temperatures 4 degrees higher than that of the D. melanogaster paralog. This study represents the first report that provides direct experimental data indicating that the evolutionary history of a species may result in adaptive changes in the structures of chaperones to enable them to elicit protective functions at extreme environments (Garbuz, 2019).

    Small heat shock proteins determine synapse number and neuronal activity during development

    Environmental changes cause stress, Reactive Oxygen Species and unfolded protein accumulation which hamper synaptic activity and trigger cell death. Heat shock proteins (HSPs) assist protein refolding to maintain proteostasis and cellular integrity. Mechanisms regulating the activity of HSPs include transcription factors and posttranslational modifications that ensure a rapid response. HSPs preserve synaptic function in the nervous system upon environmental insults or pathological factors and contribute to the coupling between environmental cues and neuron control of development. A biased screening was conducted in Drosophila melanogaster searching for synaptogenic modulators among HSPs during development. The role of two small-HSPs (sHSPs). sHSP23 and sHSP26 was explored in synaptogenesis and neuronal activity. Both sHSPs immunoprecipitate together and the equilibrium between both chaperones is required for neuronal development and activity. The molecular mechanism controlling HSP23 and HSP26 accumulation in neurons relies on a novel gene (CG1561). which was named Pinkman (pkm). It is proposed that sHSPs and Pkm are targets to modulate the impact of stress in neurons and to prevent synapse loss (Santana, 2020).

    Small heat shock protein Hsp67Bc plays a significant role in Drosophila melanogaster cold-stress tolerance

    Hsp67Bc in Drosophila melanogaster is a member of the small heat shock protein family, the main function of which is to prevent the aggregation of misfolded or damaged proteins. Hsp67Bc interacts with Starvin and Hsp23, which are known to be a part of the cold-stress response in the fly during the recovery phase. This study investigated the role of the Hsp67Bc gene in the cold-stress response. In adult Drosophila, Hsp67Bc expression was shown to increase after cold stress and decrease after 1.5 h of recovery, indicating the involvement of Hsp67Bc in short-term stress recovery. A deletion in the D. melanogaster Hsp67Bc gene was implemented using imprecise excision of a P-element, and the cold tolerance of Hsp67Bc-null mutants was analyzed at different developmental stages. Hsp67Bc-null homozygous flies were found to be viable and fertile but display varying cold-stress tolerance throughout the stages of ontogenesis: the survival after cold stress is slightly impaired in late 3(rd) instar larvae, unaffected in pupae, and notably affected in adult females. Moreover, the recovery from chill coma is delayed in Hsp67Bc-null adults of both sexes. In addition, the deletion in the Hsp67Bc gene caused more prominent up-regulation of Hsp70 following cold stress, suggesting the involvement of Hsp70 in compensation of the lack of the Hsp67Bc protein. Taken together, these results suggest that Hsp67Bc is involved in the recovery of flies from a comatose state and contributes to the protection of the fruit fly from cold stress (Malkeyeva, 2021).

    The histone replacement gene His4r is involved in heat stress induced chromatin rearrangement

    His4r is the only known variant of histone H4 in Drosophila. It is encoded by the His4r single-copy gene that is located outside of the histone gene cluster and expressed in a different pattern than H4, although the encoded polypeptides are identical. A null mutant (His4r(Δ42)) was generated which is homozygous viable and fertile without any apparent morphological defects. Heterozygous His4r(Δ42) is a mild suppressor of position-effect variegation, suggesting that His4r has a role in the formation or maintenance of condensed chromatin. Under standard conditions loss of His4r has a modest effect on gene expression. Upon heat-stress the induction of the Heat shock protein (HSP) genes Hsp27 and Hsp68 is stronger in His4r(Δ42) mutants with concordantly increased survival rate. Analysis of chromatin accessibility after heat shock at a Hsp27 regulatory region showed less condensed chromatin in the absence of His4r while there was no difference at the gene body. Interestingly, preconditioning before heat shock led to increased chromatin accessibility, HSP gene transcription and survival rate in control flies while it did not cause notable changes in His4r(Δ42). Thus, these results suggest that His4r might play a role in fine tuning chromatin structure at inducible gene promoters upon environmental stress conditions (Farago, 2021).

    Garbuz, D. G., Sverchinsky, D., Davletshin, A., Margulis, B. A., Mitkevich, V., Kulikov, A. M. and Evgen'ev, M. B. (2019). The molecular chaperone Hsp70 from the thermotolerant Diptera species differs from the Drosophila paralog in its thermostability and higher refolding capacity at extreme temperatures. Cell Stress Chaperones. PubMed ID: 31664698

    Malkeyeva, D., Kiseleva, E. and Fedorova, S. (2020). Small heat shock protein Hsp67Bc plays a significant role in Drosophila melanogaster cold-stress tolerance. J Exp Biol. PubMed ID: 32943578

    Santana, E., de Los Reyes, T. and Casas-Tinto, S. (2020). Small heat shock proteins determine synapse number and neuronal activity during development. PLoS One 15(5): e0233231, PubMed ID: 32437379

    Singh, A. K. and Lakhotia, S. C. (2016). Expression of hsromega-RNAi transgene prior to heat shock specifically compromises accumulation of heat shock-induced Hsp70 in Drosophila melanogaster. Cell Stress Chaperones 21(1): 105-120. PubMed ID: 26386576

    Sorensen, J. G., Giribets, M. P., Tarrio, R., Rodriguez-Trelles, F., Schou, M. F. and Loeschcke, V. (2019). Expression of thermal tolerance genes in two Drosophila species with different acclimation capacities. J Therm Biol 84: 200-207. PubMed ID: 31466754

    Zygotically transcribed genes

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