Gene name - eyes absent
Cytological map position - 26E1--26E4
Symbol - cli
Genetic map position - 2-17
Classification - protein tyrosine phosphatase, eyes absent family
Cellular location - nuclear
|Recent literature||Wang, P., Chen, Y., Li, C., Zhao, R., Wang, F., Lin, X., Cao, L., Li, S., Hu, L., Gao, Y., Li, Y. and Wu, S. (2015). Drosophila eye developmental defect caused by elevated Lmx1a activity is reliant on chip expression. Biochem Biophys Res Commun. PubMed ID: 26718403
The LIM-homeodomain (LIM-HD) family member Lmx1a has been successfully used to induce dopaminergic neurons from other cell types, thus showing significant implications in replacement therapies of Parkinson's disease, but the underlying mechanism remains elusive. This study used Drosophila eye as a model system to investigate how forced expression of dLmx1a (CG4328) and dLmx1a (CG32105), the fly homologs of human Lmx1a, alters cell identify. Ectopic expression of dLmx1a suppresses the formation of Drosophila eye tissue; the LIM and HD were found to be two essential domains. dLmx1a requires and physically binds to Chip, a well-known cofactor of LIM-HD proteins. Chip connects two dLmx1a proteins to form a functional tetrameric complex. In addition, evidence is provided showing that dLmx1a expression results in the suppression of two retina determination gene eyes absent (eya) and string (stg). Taken together, these findings identified Chip as a novel partner of dLmx1a to alter cell differentiation in Drosophila eye through repressing eya and stg expression, and provide an animal model for further understanding the molecular mechanism whereby Lmx1a determines cell fate.
|Jin, M. and Mardon, G. (2016). Distinct biochemical activities of Eyes absent during Drosophila eye development. Sci Rep 6: 23228. PubMed ID: 26980695
Eyes absent (Eya) is a highly conserved transcriptional coactivator and protein phosphatase that plays vital roles in multiple developmental processes from Drosophila to humans. Eya proteins contain a PST (Proline-Serine-Threonine)-rich transactivation domain, a threonine phosphatase motif (TPM), and a tyrosine protein phosphatase domain. Using a genomic rescue system, this study finds that the PST domain is essential for Eya activity and Dac expression, and the TPM is required for full Eya function. The threonine phosphatase activity plays only a minor role during Drosophila eye development and the primary function of the PST and TPM domains is transactivation that can be largely substituted by the heterologous activation domain VP16. A primary function of Eya during Drosophila eye development is as a transcriptional coactivator. Moreover, the PST/TPM and the threonine phosphatase activity are not required for in vitro interaction between retinal determination factors. Finally, this work is the first report of an Eya-Ey physical interaction. These findings are particularly important because they highlight the need for an in vivo approach that accurately dissects protein function.
|Hoi, C.S., Xiong, W. and Rebay, I. (2016). Retinal axon guidance requires integration of Eya and the JAK/STAT pathway into phosphotyrosine-based signaling circuitries in Drosophila. Genetics [Epub ahead of print]. PubMed ID: 27194748
The transcriptional coactivator and phosphatase eyes absent (Eya) is dynamically compartmentalized between the nucleus and cytoplasm. Although the nuclear transcriptional circuits within which Eya operates have been extensively characterized, understanding of its cytoplasmic functions and interactions remains limited. Previous work has showed that phosphorylation of Drosophila Eya by the Abelson tyrosine kinase can recruit Eya to the cytoplasm, and that eya-abelson interactions are required for photoreceptor axons to project to correct layers in the brain. Based on these observations, this study postulated that photoreceptor axon targeting might provide a suitable context for identifying the cytoplasmic signaling cascades with which Eya interacts. Using a dose-sensitive eya misexpression background, an RNAi-based genetic screen was performed to identify suppressors. Included among the top 10 hits are non-receptor tyrosine kinases and multiple members of the Jak/Stat signaling network (hop, Stat92E, Socs36E, and Socs44A), a pathway not previously implicated in axon targeting. Individual loss-of-function phenotypes combined with analysis of axonal projections in Stat92E null clones confirm the importance of photoreceptor autonomous Jak/Stat signaling. Experiments in cultured cells detect cytoplasmic complexes between Eya and Hop, Socs36E and Socs44A; the latter interaction requires both the Src Homology 2 motif in Socs44A and tyrosine phosphorylated Eya, suggesting direct binding and validating the premise of the screen. Taken together, these data provide new insight into the cytoplasmic phosphotyrosine signaling networks that operate during photoreceptor axon guidance and suggest specific points of interaction with Eya.
|Jin, M., Eblimit, A., Pulikkathara, M., Corr, S., Chen, R. and Mardon, G. (2016). Conditional knockout of retinal determination genes in differentiating cells in Drosophila. FEBS J [Epub ahead of print]. PubMed ID: 27257739
Conditional gene knockout in post-mitotic cells is a valuable technique which allows the study of gene function with spatiotemporal control. Surprisingly, in contrast to its long-term and extensive use in mouse studies, this technology is lacking in Drosophila. This study used a novel method for generating complete loss of eyes absent (eya) or sine oculis (so) function in post-mitotic cells posterior to the morphogenetic furrow (MF). Specifically, genomic rescue constructs with flippase recognition target (FRT) sequences flanking essential exons are used to generate conditional null alleles. By removing gene function in differentiating cells, it was shown that eya and so are dispensable for larval photoreceptor differentiation, but are required for differentiation during pupal development. Both eya and so are necessary for photoreceptor survival and the apoptosis caused by loss of eya or so function is likely a secondary consequence of inappropriate differentiation. Their requirement for cone cell development was confirmed and a novel role in interommatidial bristle (IOB) formation was revealed. In addition, so is required for normal eye disc morphology. This is the first report of a knockout method to study eya and so function in post-mitotic cells. This technology will open the door to a large array of new functional studies in virtually any tissue and at any stage of development or in adults.
|Weasner, B. M., Weasner, B. P., Neuman, S. D., Bashirullah, A. and Kumar, J. P. (2016). Retinal expression of the Drosophila eyes absent gene is controlled by several cooperatively acting cis-regulatory elements. PLoS Genet 12(12): e1006462. PubMed ID: 27930646
The eyes absent (eya) gene of the fruit fly, Drosophila melanogaster, is a member of an evolutionarily conserved gene regulatory network that controls eye formation in all seeing animals. The loss of eya leads to the complete elimination of the compound eye while forced expression of eya in non-retinal tissues is sufficient to induce ectopic eye formation. Within the developing retina eya is expressed in a dynamic pattern and is involved in tissue specification/determination, cell proliferation, apoptosis, and cell fate choice. This study explores the mechanisms by which eya expression is spatially and temporally governed in the developing eye. Multiple cis-regulatory elements function cooperatively to control eya transcription and spacing between a pair of enhancer elements is important for maintaining correct gene expression. Lastly, it was shown that the loss of eya expression in sine oculis (so) mutants is the result of massive cell death and a progressive homeotic transformation of retinal progenitor cells into head epidermis.
|Davis, T. L. and Rebay, I. (2017). Antagonistic regulation of the second mitotic wave by Eyes absent-Sine oculis and Combgap coordinates proliferation and specification in the Drosophila retina. Development 144(14):2640-2651. PubMed ID: 28619818
The transition from proliferation to specification is fundamental to the development of appropriately patterned tissues. In the developing Drosophila eye, Eyes absent (Eya) and Sine oculis (So) orchestrate the progression of progenitor cells from asynchronous cell division to G1 arrest and neuronal specification at the morphogenetic furrow. This study uncovered a novel role for Eya and So in promoting cell cycle exit in the Second Mitotic Wave (SMW), a synchronized, terminal cell division that occurs several hours after passage of the furrow. Combgap (Cg), a zinc-finger transcription factor, antagonizes Eya-So function in the SMW. Based on Cg's ability to attenuate Eya-So transcriptional output in vivo and in cultured cells and on meta-analysis of their chromatin occupancy profiles, it is speculated that Cg limits Eya-So activation of select target genes posterior to the furrow to ensure properly timed mitotic exit. This work supports a model in which context-specific modulation of transcriptional activity enables Eya and So to promote both entry into and exit from the cell cycle in a distinct spatiotemporal sequence.
The eyes absent gene, recently and more correctly termed clift, is required at an early stage in development of the compound eye. eya is not expressed in the embryonic eye primordia or in eye discs during the first larval instar, suggesting that unlike eyeless, eya is not involved in determining early commitment to eye cell fate. In fact the newly discovered mammalian eya homolog has been shown to be regulated by Pax6, the vertebrate homolog of Drosophila Eyeless (Xu, 1997). In Drosophila eya mutants, progenitor cells in the eye disc undergo programmed cell death anterior to the morphogenetic furrow, rather than proceeding along the developmental pathway leading to retinal differentiation. Thus the absence of eyes is not due to a lack of precursors but to death of those precursors. A low level of cell death normally occurs at this stage, suggesting that eya activity influences the balance between cellular differentiation and death, that is, Eya has a cell survival function. Molecular analysis identifies Eya as nuclear protein expressed in progenitor cells prior to differentiation. It thus appears that eyes absent activity is required for the survival of eye progenitor cells at a critical stage in morphogenesis (Bonini, 1993).
Besides functioning as a transcriptional co-activator that functions in conjunction with Dachshund and Sine oculis, Eyes absent is also a protein tyrosine phosphatase. It does not resemble the classical tyrosine phosphatases that use cysteine as a nucleophile and proceeds by means of a thiol-phosphate intermediate. Rather, Eyes absent is the prototype for a class of protein tyrosine phosphatases that use a nucleophilic aspartic acid in a metal-dependent reaction. Mutations that disrupt the phosphatase active site severely compromise the ability of Eyes absent to promote eye specification and development in Drosophila. The phosphatase function of Eya switches the function of Six1f-Dach, Eya interactors in mammals, from repression to activation, causing transcriptional activation through recruitment of co-activators. The gene-specific recruitment of a co-activator with intrinsic phosphatase activity provides a molecular mechanism for activation of specific gene targets, including those regulating precursor cell proliferation and survival (Li, 2003; Rayapureddi, 2003; Tootle, 2003).
The fly eyes absent (eya) gene, essential for compound eye development in Drosophila, has been shown to be functionally replaceable in eye development by a vertebrate Eya homolog. The relationship between eya and that of the eyeless gene, a Pax-6 homolog, critical for eye formation in both flies and man, has been defined: eya is essential for eyeless directed eye formation (see Specification of the eye disc primordium and establishment of dorsal/ventral asymmetry). Directing eyeless expression to discs that generate legs, wings and the antennal region of the head generates ectopic eyes in these regions. Eya is ectopically expressed in regions where eyeless directs ectopic eye formation. eya is essential for these ectopic eyes as ectopic eyes fail to form in eya mutants. eya can itself direct ectopic eye formation, indicating that eya has the capacity to function as a master control gene for eye formation. Directing ectopic eya expression to imaginal discs induces ectopic eyeless gene expression in the antennal region of the eye-antennal disc but fails to induce ectopic eyeless expression in other discs, even though ectopic ommatidia are formed and ectopic expression of Glass occurs. eya and eyeless together are more effective in eye formation than either gene alone: when expressed together, ectopic eyes are larger and form with higher penetrance than is the case with either eyeless or eya alone; expressed togther, eye formation occurs on the genitalia, a condition never observed in individuals with either gene alone. These data indicate conservation of the pathway of eya function between flies and vertebrates; they suggest a model whereby eya/Eya gene function is essential for eye formation by eyeless/Pax-6, and that eya/Eya can in turn mediate, via a regulatory loop, the activity of eyeless/Pax-6 in eye formation (Bonini, 1997).
eyes absent also plays a role in the embryonic determination of somatic gonadal precursor (SGP) cell fate. The gonad forms from cells of two lineages: the germline and soma (see gonadogenesis and oogenesis for more information). The somatic gonadal cells generate the various cell types within the testis or ovary that support gametogenesis.
It may be useful here to provide a brief description of the origin of SGP cells: each of the somatic cell types of the gonad arises from mesodermal cells that constitute the embryonic gonad. Using markers for the precursors of the somatic cells of the gonad, five discrete steps have been identified in gonadal development:
The functions of the homeotic genes abdominal A and Abdominal B are both required for the development of gonadal precursors. Each plays a distinct role. abd A activity alone specifies anterior gonadal precursor fates, whereas abd A and Abd B act together to specify a posterior subpopulation of gonadal precursors. Once specified, gonadal precursors born within posterior parasegments move to the site of gonad formation. The proper regional identities, as established by homeotic gene function, are required for the arrest of migration at the correct position. abd A is required in a population of cells within parasegments 10 and 11 that partially ensheath the coalescing gonad. Mutations in iab-4, a distal enhancer element, abolish expression of abd A within these cells, blocking the coalescence of the gonad (Boyle, 1995).
clift has been identified as a regulator of Drosophila gonadogenesis. When cloned, clift turned out to be identical to eyes absent. Mutations in clift abolish gonad formation and produce female sterility. In addition to showing an eye and gonad phenotype, some clift alleles show reduced or absent ocelli and abnormal morphology of the adult brain (Nusslein-Volhard, 1984). Just as with abdominal A, clift is expressed within SGP as these cells first form, demonstrating that 9-12 cells are selected as SGP within each of three posterior parasegments at early stages in gonadogenesis. In abdominal A mutants, clift fails to be expressed, and in abd A overexpressors, clift is expressed ectopically. Despite the early expression of clift, SGP's are specified in the absence of clift function. However, they fail to maintain their fate. Consequently, germ cells do not coalesce into a gonad. In addition, using clift as a marker, it has been shown that the anteroposterior and dorsoventral position of the somatic gonadal precursor cells within a parasegment are established by the secreted growth factor Wingless, acting from the ectoderm, coupled with a gene regulatory hierarchy involving abd A within the mesoderm. While loss of wg abolishes gonadal precursors, ectopic expression expands the population such that most cells within lateral mesoderm adopt gonadal precursor fates. Initial dorsoventral positioning of somatic gonadal precursors relies on a regulatory cascade that establishes dorsal fates within the mesoderm. tinman appears to mediate the role of ectodermally expressed decapentaplegic; in tinman mutants few or no SGP cells are detected. clift expression is subsequently refined through negative regulation by bagpipe, a gene that specifies nearby visceral mesoderm. Thus, these studies identify essential regulators of gonadal precursor specification and differentiation and reveal novel aspects of the general mechanism whereby somatic gonadal cell fate is allocated within the mesoderm (Boyle, 1997).
Does clift/eyes absent have the same targets in the eye that it has in somatic gonad precursors? Is there a unified role for this gene in the two organs? In the eye, eyes absent is implicated in cell survival, while in the gonads clift is implicated in gonadal cell fate. One clift mutant, which acts as a strong allele in gonadal fate determination, nevertheless produces a transcript. Use of this mutant allowed an examination of SGP cell development in the absence of clift function. Shortly after SGP cells are specified in cli mutants there is a reduction in the number of cli expressing SGP cells, such that by germ band retraction, few SGP cells remain. The few cells that remain fail to complete their migration to the position of gonad formation (Boyle, 1997). It would therefore appear that there might be a role for clift in SGP survival in the mesoderm as there is for eya in photoreceptor precursor survival in the eye. The answer to whether there is a unified role for eya/clift in eyes and in the mesoderm awaits discovery of the target(s) for this gene.
Drosophila eye specification and development relies on a collection of transcription factors termed the retinal determination gene network (RDGN). Two members of this network, Eyes absent (EYA) and Sine oculis (SO), form a transcriptional complex in which EYA provides the transactivation function while SO provides the DNA binding activity. EYA also functions as a protein tyrosine phosphatase, raising the question of whether transcriptional output is dependent or independent of phosphatase activity. To explore this, microarrays together with binding site analysis, quantitative real-time PCR, chromatin immunoprecipitation, genetics and in vivo expression analysis were used to identify new EYA-SO targets. In parallel, the expression profiles of tissue expressing phosphatase mutant eya were examined, and it was found that reducing phosphatase activity did not globally impair transcriptional output. Among the targets identified by this analysis was the cell cycle regulatory gene, string (stg), suggesting that EYA and SO may influence cell proliferation through transcriptional regulation of stg. Future investigation into the regulation of stg and other EYA-SO targets identified in this study will help elucidate the transcriptional circuitries whereby output from the RDGN integrates with other signaling inputs to coordinate retinal development (Jemc, 2007).
Two general conclusions have resulted from this work: (1) the similarity in expression profiles between tissue overexpressing wild type and phosphatase-dead eya transgenes and analysis of gene expression by quantitative PCR suggest that EYA's phosphatase is not generally required for EYA transcriptional activity, although it may be required for maximal transactivation of some target genes, and (2) the short sequence (T/C/G)GA(A/T/G)A(T/C) appears to be the only recognizable motif shared among all SO binding sites. As exemplified by in vivo validation of EYA-SO-mediated regulation of the cell cycle regulator stg, further analysis of the target genes identified in this study will likely shed new light into the mechanisms underlying EYA-SO function during development (Jemc, 2007).
The main goal of this study was to identify new targets of EYA transcriptional activity. Although adult head tissue was used for overexpression experiments, an 86% success rate in confirming changes in expression of potential targets in developing Drosophila eye-antennal imaginal discs overexpressing eya supports the ability of this system to identify similar data sets in different developmental stages. Out of the ten genes upregulated by eya overexpression in both adult head tissue and eye-antennal imaginal discs, five demonstrated enrichment of endogenous SO at one or more predicted binding sites. These predicted binding regions were conserved across a minimum of two and up to nine Drosophila species, emphasizing their likely biological relevance. Two binding sites that did not demonstrate SO enrichment were not conserved across other Drosophila species, while binding sites in the remaining three genes were conserved across multiple species and could be EYA-SO targets in other tissues (Jemc, 2007).
The core sequence shared by all of these targets is (T/C/G)GA(A/T/G)A(T/C), a pared down version of the previously proposed GTAAN(T/C)NGANA(T/C)(C/G) SO binding sequence. In Drosophila EYA-SO targets, the sequence flanking the core (T/C/G)GA(A/T/G)A(T/C) has been shown to be important only in the case of the target so, and is absent in one of the two SO binding sites identified in the lz locus and the binding sites in stg were confirmed by gel shifts. While specific flanking sequences may further stabilize SO-DNA interactions, characterization of such a flanking sequence consensus awaits further analysis. Confirmation of additional targets predicted by microarray and binding site analysis should provide for further characterization of the SO binding sequence. Out of the remaining 31 potential targets, all except one have binding sites conserved across multiple Drosophila species, suggesting that additional EYA-SO targets will be confirmed within this data set (Jemc, 2007).
While none of the previously identified EYA-SO targets were included in the final list, two targets, so and lz were upregulated upon eya overexpression, although less than the two-fold cutoff. The expression of the previously identified targets hh, ato and ey was either absent or changes were not statistically significant. One explanation for this observation is that other signaling pathways required for the expression of these genes may not be activated, or, conversely, inhibitory signaling pathways could be activated in adult head tissue (Jemc, 2007).
In addition to examining expression levels of previously identified EYA-SO targets, the list of upregulated EYA-SO target candidates was compared to genes that were upregulated by ey overexpression in microarray analyses. Because ey both induces eya and so expression and is itself transcriptionally regulated by EYA-SO, one would expect to see a number of genes similarly regulated by overexpression of either ey or eya; however, as detailed below, pairwise comparisons between the current data set to lists of candidate ey targets derived from two independent array studies, reveals a surprisingly limited overlap. One study identified 371 genes with at least 1.5-fold upregulation across two array experiments, only 55 of which were similarly upregulated in both arrays. Comparison of this data set to a second more recent report of 300 candidate genes upregulated in response to ey overexpression revealed only 24 common targets. Comparison to the current list of potential eya-so targets yielded 10 shared with the first data set and 3 common to the second results. Encouragingly, despite this limited overlap, stg, a gene shown in this study to be transcriptionally regulated by eya and so, was one of the two targets consistently upregulated in all three studies (Jemc, 2007).
As additional targets are confirmed, it is important to note that EYA may also associate with transcription cofactors other than SO to regulate gene expression. Although EYA can associate with DAC, and X-ray crystallographic analysis suggests DAC can bind DNA, targets of an EYA-DAC complex or a consensus DAC binding site have not been identified. In addition, EYA also contains an engrailed homology 1 (eh1) domain, suggesting it may be able to bind to the transcriptional repressor Groucho (GRO). However, as current in vivo data only supports a role for EYA as a transactivator complexed to SO, identification of additional EYA cofactors in vivo will be necessary to explore the potential of SO-independent EYA transcriptional functions further (Jemc, 2007).
Many of the genes identified as direct EYA-SO transcriptional targets are largely uncharacterized 'CGs' whose expression patterns in the eye will have to be studied in detail to gain further insight to EYA-SO-mediated regulation, but a few have predicted or well-studied functions that may provide insight into how EYA-SO functions during normal development and how misregulation can result in disease. Most notable on this list is stg. Given that overexpression of eya and so results in overproliferation, while their loss leads to tissue reduction, EYA-SO control of stg expression provides a mechanism for how EYA-SO regulation of the cell cycle may in turn affect cell proliferation. An interesting question for future investigation is how the relatively broad expression of EYO and SO throughout the developing retina activates stg expression only in a relatively narrow stripe of cells just anterior to the morphogenetic furrow. Given the apparent complexity of stg cis-regulatory elements, a likely explanation is that EYA-SO act combinatorially with transcriptional effectors of other signaling pathways to effect this developmental precision (Jemc, 2007).
Consistent with eya and so overexpression leading to increased tissue overgrowth in Drosophila, elevated levels of Eya and Six family members have been observed in a variety of cancers. Studies of the transcriptional targets of mammalian Eya and SO/Six proteins have identified the cell cycle regulatory genes, cyclin D1 and cyclin A1, the proto-oncogene c-Myc and ezrin, a regulator of the cytoskeleton and contributor to metastasis, suggesting intermediates through which Eya and Six family genes regulate proliferation and contribute to cancer. Identification of stg as a transcriptional target of EYA and SO in Drosophila provides not only the first direct cell cycle target in Drosophila, but also suggests another target through which EYA and SO might regulate proliferation in other organisms (Jemc, 2007).
Before parallels can be drawn to how EYA-SO targets important for Drosophila retinal development might be relevant to development and disease in other organisms, it will be necessary to examine the conservation of the transcriptional regulatory circuits. However, given the predicted functions of the gene products encoded by candidate EYA-SO targets, together with knowledge of Eya-Six function in mammalian systems, it is tempting to speculate. For example, CG12030, the Drosophila homolog of the human Gale, encodes a sugar epimerase required for galactose metabolism. As metabolic abnormalities have been demonstrated to play a part in cataract formation, and mutations in eya have been observed in patients with congenital cataracts, the identification of CG12030 as an EYA-SO target suggests intermediates through with eya might function to maintain homeostasis in the eye. Mal, which encodes a molybdenum cofactor sulfurase important for ommochrome biosynthesis, is expressed in Drosophila pigment cells in the eye and would seem a logical target of the RDGN. Mutations of the human homolog of mal, HMCS, can result in renal failure and myositis, both intriguing phenotypes given the importance of Eya-Six in vertebrate kidney and muscle development. CG15879 encodes the Drosophila homolog of human SERHL2, a member of a serine hydrolase-like family predicted to regulate muscle growth, a developmental context in which Eya and Six family genes function in Drosophila and vertebrates. Lastly, CG8449 has a predicted RabGAP/TBC domain. While RabGAPs function in a variety of developmental contexts, RabGAP-like proteins have been predicted to function in phototransduction and synaptic transmission in Drosophila and mutations in RabGAP genes have been isolated in cases of Warburg Micro syndrome, a severe autosomal recessive disorder characterized by abnormalities in the eye, as well as the central nervous system and genitals, all contexts in which Drosophila eya and so are expressed. Identification of additional EYA-SO targets and the examination of the conservation of EYA-SO transcriptional regulation across homologous genes in different species will be necessary to determine how EYA and contribute to development and disease (Jemc, 2007).
Given the importance of achieving appropriate levels of gene expression during the course of development, it is not surprising that multiple signaling pathways converge to regulate common target genes at the level of transcription. For example, hh and lz are coordinately regulated by receptor tyrosine kinase (RTK) downstream effectors of the ETS family in conjunction with EYA and SO. This study has identified stg as an EYA-SO target and suggests stg transcription is also regulated Notch and Wingless (Wg) signaling, and by RTK signaling. Thus, these results suggest a mechanism by which members of the RDGN are integrated with Notch and Wg signaling to coordinate cell proliferation. Identification of additional EYA-SO targets is likely to reveal new nodes for integration of the RDGN with other signaling pathways, explaining how signaling pathways cooperate to yield specific developmental outcomes (Jemc, 2007).
Bases in 5' UTR - 395 for type 1 and 210 for type II
Exons - 5 for each transcript
Bases in 3' UTR - 553
There are two identified cDNA sequences initiating from different promoters. The first 19 amino acids of the type I cDNA and the first 25 of the type II are generated by alternative splicing; these transcripts differ in their first exons (Bonini, 1993).
Comparison of the vertebrate proteins with Drosophila Eya reveals several domains of homology. The largest domain is the C-terminal portion of the protein, referred to as ED1 (for Eya homology domain1), which shows 53% identity over 271 amino acids in all the vertebrate and fly clones. The vertebrate ED1 sequences are more similar to one another than to the fly's ED1 sequence. Within the N-terminal domain, the sequences show little conservation. A short region of weak homology, ED2, occurs between the vertebrate and fly sequences in the amino terminus. In this domain, a run of spaced tyrosine residues is conserved. The fly sequence has a weak PEST protein degredation sequence; in a similar location, the mouse Eya2 sequence has a region that may serve as a weak PEST site. The fly Eya protein sequence has a consensus nuclear localization sequence at the amino terminus, and the protein is nuclear by immunocytochemistry. However, none of the vertebrate sequences show a motif indicative of a nuclear localization sequence (Zimmerman, 1997). The Drosophila protein has several potential cyclic nucleotide-dependent, protein kinase C and tyrosine kinase phosphorylation sites (Bonini, 1993)
The retinal determination (RD) gene network encodes a group of transcription factors and cofactors necessary for eye development. Transcriptional and posttranslational regulation of RD family members is achieved through interactions within the network and with extracellular signaling pathways, including epidermal growth factor receptor/RAS/mitogen-activated protein kinase (MAPK), transforming growth factor beta/DPP, Wingless, Hedgehog, and Notch. A structure-function analyses reveals novel aspects of Eyes absent (Eya) function and regulation. The conserved C-terminal Eya domain negatively regulates Eya transactivation potential, and Groucho-Sine oculis (So) interactions provide another mechanism for negative regulation of Eya-So target genes. The transactivation potential of Eya maps to an internal proline-, serine-, and threonine-rich region that includes the Eya domain 2 (ED2) and two MAPK phosphorylation consensus sites and demonstrate that activation of the RAS/MAPK pathway potentiates transcriptional output of Eya and the Eya-So complex in certain contexts. Drosophila S2 cell two-hybrid assays were used to describe a novel homotypic interaction that is mediated by Eya's N terminus. These data suggest that EYA requires homo- and hetero-typic interactions and RAS/MAPK signaling responsiveness to ensure context-appropriate RD gene network activity (Silver, 2003).
date revised: 20 November 2003
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