Moesin : Biological Overview | Evolutionary Homologs | Regulation | Developmental Biology | Effects of Mutation | References
Gene name - Moesin

Synonyms - Moesin-like, DMoesin

Cytological map position - 8B4--6

Function - signaling

Keywords - oogenesis, posterior group, cortical actin assembly, apical-basal polarity

Symbol - Moe

FlyBase ID: FBgn0011661

Genetic map position - X

Classification - second domain of FERM, Moesin tail domain, PH domain-like, ubiquitin-like

Cellular location - cytoplasmic

NCBI link: Entrez Gene
Moe orthologs: Biolitmine
Recent literature
Sherrard, K.M. and Fehon, R.G. (2015). The transmembrane protein Crumbs displays complex dynamics during follicular morphogenesis and is regulated competitively by Moesin and aPKC. Development 142(10):1869-78. PubMed ID: 25926360
The transmembrane protein Crumbs (Crb) functions in apical polarity and epithelial integrity. To better understand its role in epithelial morphogenesis, this study examined Crb localization and dynamics in the late follicular epithelium of Drosophila. Crb was unexpectedly dynamic during middle-to-late stages of egg chamber development, being lost from the marginal zone (MZ) in stage 9 before abruptly returning at the end of stage 10b, then undergoing a pulse of endocytosis in stage 12. The reappearance of MZ Crb was necessary to maintain an intact adherens junction and MZ. Although Crb has been proposed to interact through its juxtamembrane domain with Moesin (Moe), a FERM domain protein that regulates the cortical actin cytoskeleton, the functional significance of this interaction is poorly understood. This study found that whereas the Crb juxtamembrane domain was not required for adherens junction integrity, it was necessary for MZ localization of Moe, aPKC and F-actin. Furthermore, Moe and aPKC functioned antagonistically, suggesting that Moe limits Crb levels by reducing its interactions with the apical Par network. Additionally, Moe mutant cells lost Crb from the apical membrane and accumulated excess Crb at the MZ, suggesting that Moe regulates Crb distribution at the membrane. Together, these studies reveal reciprocal interactions between Crb, Moe and aPKC during cellular morphogenesis.

Panneton, V., Nath, A., Sader, F., Delaunay, N., Pelletier, A., Maier, D., Oh, K. and Hipfner, D. R. (2015). Regulation of catalytic and non-catalytic functions of the Drosophila Ste20 kinase Slik by activation segment phosphorylation. J Biol Chem [Epub ahead of print]. PubMed ID: 26170449
Protein kinases carry out important functions in cells both by phosphorylating substrates and by means of regulated non-catalytic activities. Such non-catalytic functions have been ascribed to many kinases, including some members of the Ste20 family. The Drosophila Ste20 kinase Slik phosphorylates and activates Moesin in developing epithelial tissues to promote epithelial tissue integrity. It also functions non-catalytically to promote epithelial cell proliferation and tissue growth. A structure-function analysis was carried out to determine how these two distinct activities of Slik are controlled. The conserved C-terminal coiled-coil domain (CCD) of Slik, which is necessary and sufficient for apical localization of the kinase in epithelial cells, is not required for Moesin phosphorylation but is critical for the growth-promoting function of Slik. Slik is auto- and trans-phosphorylated in vivo. Phosphorylation of at least two of three conserved sites in the activation segment is required for both efficient catalytic activity and non-catalytic signaling. Slik function is thus dependent upon proper localization of the kinase via the CCD and activation via activation segment phosphorylation, which enhances both phosphorylation of substrates like Moesin and engagement of effectors of its non-catalytic growth-promoting activity.
Kristo, I., Bajusz, C., Borsos, B. N., Pankotai, T., Dopie, J., Jankovics, F., Vartiainen, M. K., Erdelyi, M. and Vilmos, P. (2017). The actin binding cytoskeletal protein Moesin is involved in nuclear mRNA export. Biochim Biophys Acta [Epub ahead of print]. PubMed ID: 28554770
Current models imply that the evolutionarily conserved, actin-binding Ezrin-Radixin-Moesin (ERM) proteins perform their activities at the plasma membrane by anchoring membrane proteins to the cortical actin network. This study shows that beside its cytoplasmic functions, the single ERM protein of Drosophila, Moesin, has a novel role in the nucleus. The activation of transcription by heat shock or hormonal treatment increases the amount of nuclear Moesin, indicating biological function for the protein in the nucleus. The distribution of Moesin in the nucleus suggests a function in transcription and the depletion of mRNA export factors Nup98 or its interacting partner, Rae1, leads to the nuclear accumulation of Moesin, suggesting that the nuclear function of the protein is linked to mRNA export. Moesin localizes to mRNP particles through the interaction with the mRNA export factor PCID2 and knock down of Moesin leads to the accumulation of mRNA in the nucleus. Based on these results it is proposed that, beyond its well-known, manifold functions in the cytoplasm, the ERM protein of Drosophila is a new, functional component of the nucleus where it participates in mRNA export.
Abeysundara, N., Simmonds, A. J. and Hughes, S. C. (2017). Moesin is involved in polarity maintenance and cortical remodelling during asymmetric cell division. Mol Biol Cell [Epub ahead of print]. PubMed ID: 29282284
An intact actomyosin network is essential for anchoring polarity proteins to the cell cortex and maintaining cell size asymmetry during asymmetric cell division of Drosophila neuroblasts. However, the mechanisms that control changes in actomyosin dynamics during asymmetric cell division remain unclear. This study finds that the actin-binding protein, Moesin, is essential for neuroblast proliferation and mitotic progression in the developing brain. During metaphase, phosphorylated Moesin (p-Moesin) is enriched at the apical cortex and loss of Moesin leads to defects in apical polarity maintenance and cortical stability. This asymmetric distribution of p-Moesin is determined by components of the apical polarity complex and Slik kinase. During later stages of mitosis, p-Moesin localization shifts more basally, contributing to asymmetric cortical extension and myosin basal furrow positioning. These findings reveal Moesin as a novel apical polarity protein that drives cortical remodelling of dividing neuroblasts, which is essential for polarity maintenance and initial establishment of cell size asymmetry.
Plutoni, C., Keil, S., Zeledon, C., Delsin, L. E. A., Decelle, B., Roux, P. P., Carreno, S. and Emery, G. (2019). Misshapen coordinates protrusion restriction and actomyosin contractility during collective cell migration. Nat Commun 10(1): 3940. PubMed ID: 31477736
Collective cell migration is involved in development, wound healing and metastasis. In the Drosophila ovary, border cells (BC) form a small cluster that migrates collectively through the egg chamber. To achieve directed motility, the BC cluster coordinates the formation of protrusions in its leader cell and contractility at the rear. Restricting protrusions to leader cells requires the actin and plasma membrane linker Moesin. This study shows that the Ste20-like kinase Misshapen phosphorylates Moesin in vitro and in BC. Depletion of Misshapen disrupts protrusion restriction, thereby allowing other cells within the cluster to protrude. In addition, this study shows that Misshapen is critical to generate contractile forces both at the rear of the cluster and at the base of protrusions. Together, these results indicate that Misshapen is a key regulator of BC migration as it coordinates two independent pathways that restrict protrusion formation to the leader cells and induces contractile forces.

Two prominent characteristics of epithelial cells, apical-basal polarity and a highly ordered cytoskeleton, depend on the existence of precisely localized protein complexes associated with the apical plasma membrane and on a separate machinery that regulates the spatial order of actin assembly. ERM (ezrin, radixin, moesin) proteins have been proposed to link transmembrane proteins to the actin cytoskeleton in the apical domain, suggesting a structural role in epithelial cells, and they have been implicated in signalling pathways. The sole Drosophila ERM protein Moesin functions to promote cortical actin assembly and apical-basal polarity. As a result, cells lacking Moesin lose epithelial characteristics and adopt invasive migratory behaviour. These data demonstrate that Moesin facilitates epithelial morphology not by providing an essential structural function, but rather by antagonizing activity of the small GTPase Rho. Thus, Moesin functions in maintaining epithelial integrity by regulating cell-signalling events that affect actin organization and polarity. Furthermore, these results show that there is negative feedback between ERM activation and activity of the Rho pathway (Speck, 2003).

ERM proteins, highly related members of the larger protein 4.1 superfamily, can exist in an active or inactive conformation. The inactive conformation is brought about by head-to-tail interactions between the FERM (four-point-one, ERM) domain and the carboxy terminus. In this state, ERM actin-binding and membrane association sites are not accessible. A conformational change induced by Rho pathway activation produces the active ERM form. Mammalian ERM proteins bind actin and cell-type-dependent transmembrane proteins such as CD44, CD43 and several I-CAMs. Genetic tests of hypotheses regarding ERM protein involvement in signalling and cytoskeletal support have been impeded by functional redundancies between these three proteins in mammals. In contrast, Drosophila has only one ERM gene, Moesin, enabling such analyses to be performed (Speck, 2003).

To test Moesin function in vivo, a transposable element insertion in the Moesin gene, P{lacW}l(1)G0323, was identified. Males hemizygous for this insertion display late larval lethality. This lethality can be rescued by a duplication of region 8B, Dp(1;Y)619, as well as by a complementary DNA-based Moesin+ transgene. Taken together, these results indicate that l(1)G0323 disrupts Moesin function but does not affect any other essential genes, and is hereafter referred to as MoeG0323 (Speck, 2003).

To determine the extent to which Moesin function is disrupted by this allele, Moesin protein level and distribution were examined in MoeG0323 mutants. Moesin protein is not detected in imaginal discs from MoeG0323 hemizygous larvae by immunofluorescence or immunoblotting. Furthermore, animals trans-heterozygous for the MoeG0323 allele and a deficiency of the 8B region, Df(1)lz-90b24, display the same lethal period and have phenotypes identical to MoeG0323 hemizygous and homozygous mutant larvae, establishing that MoeG0323 is genetically null (Speck, 2003).

To investigate Moesin function in the morphogenesis of epithelial structures the imaginal discs were examined from MoeG0323 hemizygous larvae. In wild-type epithelial cells, filamentous actin associates with the cell cortex and accumulates in the apical domain, where it largely coincides with Moesin. In MoeG0323 cells, F-actin undergoes a marked redistribution: it accumulates in ectopic sites within cells and becomes depleted from the apical region. To demonstrate that actin is reduced in the apical domain, a Moe+ transgene was examined only in the posterior compartment of the mutant disc, generating a 'half-rescued' disc. The rescued cells in the posterior compartment have a wild-type phenotype, and have significantly more filamentous actin in the apical domain than adjacent MoeG0323 cells (Speck, 2003).

The imaginal epithelium normally consists of a single layer of tall columnar cells with an obvious apical-basal polarity. In MoeG0323 imaginal discs, many cells are situated basal to the epithelial monolayer. These cells do not express the junctional marker E-Cadherin or other epithelial polarity markers, including ß-Catenin, Coracle, Crumbs or Discs lost. These data suggest that in MoeG0323 organisms, epithelial cells lose intercellular junctions and epithelial polarity, and are extruded basally from the epithelium (Speck, 2003).

To determine whether cells lacking Moesin not only lose epithelial morphology but also adopt migratory behaviour, a subset of cells within the imaginal epithelium was marked by expressing green fluorescent protein (GFP) under control of the region-specific patched-Gal4 driver. In a wild-type wing imaginal disc, marked cells stay within a continuous stripe along the anterior-posterior boundary. In a MoeG0323 disc, cells expressing GFP under control of patched-Gal4 are found away from the stripe, suggesting that these cells have dispersed. Furthermore, these cells are able to invade adjacent regions of the disc comprising cells that still retain epithelial morphology. Thus, MoeG0323 cells not only lose epithelial markers and intercellular adhesion, but also become motile and show invasive behavior, suggesting that cells lacking Moesin undergo an epithelial-to-mesenchymal transition (Speck, 2003).

Rho-induced phosphorylation of a C-terminal threonine has been shown to relieve the inhibitory intramolecular association and generate the active conformation of the ERM molecule (Matsui, 1998; Shaw, 1998; Simons, 1998). This residue is conserved in Drosophila Moesin at amino acid position 559. To test the role of Thr 559 phosphorylation in Moesin activation, two mutant cDNA constructs were generated: Moe(T559D), a phosphomimetic form, and Moe(T559A), a form that cannot be phosphorylated at this residue. MoeG0323 hemizygous males are rescued to viability by transgenes expressing Moe(T559D), but not by Moe(T559A), implying that Moe(T559D) is an active form whereas Moe(T559A) is inactive (Speck, 2003).

The phosphomimetic form of ERM proteins has constitutively active properties because it may be locked in the open conformation (Gautreau, 2000). To examine the effects of ERM activation, Moe(T559D) was overexpressed under region-specific Gal4 drivers in wild-type imaginal discs. A marked upregulation of cortical F-actin was observed in cells that express Moe(T559D). By contrast, little or no effect was seen with Moe+ and Moe(T559A) overexpression. The accumulation of cortical actin induced by the gain-of-function mutant is complementary to the reduction of apical actin seen in the Moesin loss-of-function background, indicating that Moesin controls assembly of cortical actin (Speck, 2003).

The pleiotropic nature of the MoeG0323 phenotype suggests an involvement in a signalling pathway that controls cell adhesion and motility. Given the crucial function carried out by the Rho family of small GTPases in regulating these processes and the evidence for Rho regulation of ERM function (Shaw; 1998; Hirao; 1996; Mackay, 1997) the relationship between Moesin and Rho signalling in vivo was examined by manipulating the genetic dose of Rho1, a Drosophila RhoA homolog, in a Moe- background. The null Rho172R allele was used to reduce by half maternal and zygotic Rho function. Halving the dose of Rho1 not only ameliorates the MoeG0323 disc epithelium organization and actin localization phenotypes, but also strongly suppresses MoeG0323 lethality. A similar suppression of lethality was observed with other Rho1 alleles and when the dose of Drok, a Rho effector kinase, was reduced, and a weaker effect was observed with another downstream effector of Rho1 signalling, the non-muscle myosin-II heavy chain gene zipper). The observed suppression was not due to enhanced Moesin protein production or stability. Taken together, these results suggest that Moesin functions antagonistically to activity of the Rho pathway in regulating epithelial polarity and integrity (Speck, 2003).

The ultrastructure was examined of wild-type and MoeG0323 epithelial cells as well as that of MoeG0323 cells that are also heterozygous for Rho172R. In transmission electron micrographs, wild-type epithelial cells have apical finger-like microvilli that tend to congregate near the areas of contact between adjacent cells. In the Moesin mutant, the microvilli are replaced by large apical protrusions that are often of irregular shape. Reduction of Rho activity partially suppresses this Moe- phenotype, and results in cells that lack the protrusions but still display abnormally large microvillus-like structures. These results suggest that Moesin may have a Rho-independent role in maintaining apical integrity that is not essential for viability (Speck, 2003).

If Moesin normally functions as an antagonist of Rho pathway activity, then the effects of Rho pathway hyperactivation should be similar to the Moe loss-of-function phenotype. To test this hypothesis, whether Rho1 overexpression phenotypes in wing imaginal discs mimic those seen in Moe loss-of-function mutants was investigated. Cells overexpressing Rho1 mislocalize F-actin and lose epithelial characteristics. As with MoeG0323 cells, Rho1-expressing cells in the blade region of the wing imaginal disc lose junctional markers and drop basally. These cells also assume a mesenchymal character, become motile and invade between wild-type epithelial cells. Similar, but more disruptive phenotypes were observed when a constitutively active form of Rho1, Rho1V14, was overexpressed in wild-type imaginal discs. Therefore, Rho1 overexpression strongly resembles Moe loss of function, suggesting that Moesin and Rho1 exert opposite effects on the epithelium (Speck, 2003).

To test further the relationship of Moesin with the Rho pathway, whether a reduction in Moe function would suppress a phenotype associated with downregulation of Rho1 was investigated. Rho1 has been shown to function downstream of dishevelled (dsh) in the planar cell polarity (PCP) pathway that regulates the polarity and number of hairs generated by each wing blade cell. Each wild-type wing cell produces a single hair; however, multiple wing hairs result when Rho pathway function is impaired. In flies that are mutant for the dsh1 allele (which inactivates the PCP but not the wingless-signalling function of dishevelled), double-hair-producing cells occurred at a frequency of 6.3%. Removal of a single dose of Moe suppresses the number of cells with double hairs in dsh1 mutants to 1.2%, suggesting that Rho pathway function is upregulated in response to reduction in Moesin activity (Speck, 2003).

These genetic results in Drosophila suggest that Moesin functions antagonistically to Rho pathway activity. To distinguish between an effect on Rho itself and effects on a downstream component of the pathway, and to extend these results from Drosophila to mammalian cells, the effect of reducing ERM function in mammalian LLC-PK1 epithelial cells was examined. Use was made of an ERM truncation, ezrinAct, that has dominant-negative properties in flies and reduces function of all three ERM proteins in these cells (as assayed by phospho-ERM staining, a marker for ERM activation (Hayashi, 1999). Expression of ezrinAct resulted in increased levels of Rho activity, whereas expression of wild-type ezrin caused either no effect, or slightly decreased the level of Rho activity. These results indicate that ERM proteins negatively regulate Rho pathway function by altering the activation state of Rho itself, rather than a downstream component of the pathway (Speck, 2003).

The phenotypic analysis presented in this study clearly demonstrates that Moesin function is required to maintain apical-basal polarity, epithelial integrity, and correct actin distribution. This is consistent with Moesin's association with the apical membrane in epithelial cells and the ability to bind filamentous actin (McCartney; 1996; Edwards, 1997). These results also show that Moesin promotes epithelial morphology by functioning antagonistically to Rho pathway activity, rather than by providing structural linkage between the plasma membrane and the cytoskeleton. Moe- defects, including lethality, are strongly suppressed when Rho pathway activity is reduced, suggesting that Moesin does not have essential structural functions. Furthermore, the diverse Moe- phenotypes -- decreased cortical actin accumulation, loss of epithelial polarity, and acquisition of migratory cell behavior -- can all be attributed to increased Rho pathway activity. Consistent with this model for Moesin function, increased Rho activity has been linked to invasive and metastatic phenotypes in mammalian epithelial cells. Because ERM proteins are known downstream effectors of Rho signalling, the results further suggest the existence of a negative feedback loop between ERM proteins and the Rho pathway. This negative feedback mechanism may be important in inhibiting migratory and invasive cellular behaviors that characterize metastatic cells (Speck, 2003).

The results with mammalian epithelial cells suggest that the effect of ERM function on Rho signalling occurs upstream to or at the level of Rho itself. Previous studies have detected direct binding between ERM proteins and two known regulators of Rho function, RhoGDI (Rho GDP dissociation inhibitor: Takahash, 1997) and Dbl GEF (guanine nucleotide exchange factor for Rho: Takahashi, 1998). Although one of these studies suggested that ERM proteins function to promote Rho activation in vitro (Takahashi, 1997), the in vivo significance of these interactions has not yet been examined. Elucidation of the functional significance of these interactions may be essential to the further understanding of the relationship between ERM proteins and Rho pathway activity. Of note, the proposed negative feedback loop between ERM and Rho parallels the antagonistic relationship between the ERM-related tumour suppressor Merlin and another Rho-family GTPase Rac (Shaw, 2001), and suggests that ERM and Merlin may have similar roles in regulating Rho-family signalling (Speck, 2003).

Crumbs, Moesin and Yurt regulate junctional stability and dynamics for a proper morphogenesis of the Drosophila pupal wing epithelium

The Crumbs (Crb) complex is a key epithelial determinant. To understand its role in morphogenesis, this study examined its function in the Drosophila pupal wing, an epithelium undergoing hexagonal packing and formation of planar-oriented hairs. Crb distribution is dynamic, being stabilized to the subapical region just before hair formation. Lack of crb or stardust, but not DPatj, affects hexagonal packing and delays hair formation, without impairing epithelial polarities but with increased fluctuations in cell junctions and perimeter length, fragmentation of adherens junctions and the actomyosin cytoskeleton. Crb interacts with Moesin and Yurt, FERM proteins regulating the actomyosin network. Moesin and Yurt distribution at the subapical region depends on Crb. In contrast to previous reports, yurt, but not moesin, mutants phenocopy crb junctional defects. Moreover, while unaffected in crb mutants, cell perimeter increases in yurt mutant cells and decreases in the absence of moesin function. These data suggest that Crb coordinates proper hexagonal packing and hair formation, by modulating junction integrity via Yurt and stabilizing cell perimeter via both Yurt and Moesin. The Drosophila pupal wing thus appears as a useful system to investigate the functional diversification of the Crb complex during morphogenesis, independently of its role in polarity (Salis, 2017).

This study aimed at unveiling the function of the Crumbs complex in epithelial morphogenesis. Although Crb was discovered several decades ago in Drosophila, the severe apico-basal polarity defects associated to crb inactivation in embryos have hampered the full exploration of its function during epithelia development. The results indicate that Crb also acts during pupal wing morphogenesis, where the absence of crb function does not impair AP/BL polarity and does not lead to the dramatic tissue alterations often seen in other tissues. The pupal wing thus represents an attractive model system, well suited to dissect additional functions of the Crb complex during epithelial morphogenesis, independently of its role in polarity (Salis, 2017).

The redistribution of Crb at the subapical region (SAR) at the end of hexagonal packing, as well as the defects in cells orientation observed in crb mutants suggest that Crb is required to stabilize the actin cytoskeleton and E-cadherin at the adherens junctions at the end of tissue rearrangement. Alterations in F-actin and Myosin II (Myo) distribution in crb mutant cells strikingly mimic those observed in embryos mutant for the actin-binding protein Canoe/Afadin, which links the actomyosin network to AJs. Canoe loss diminishes this coupling leading to reduced cell shape anisometry and defects in germ band elongation. As for crb, canoe mutant cells still retain some ability to change their shape and germ band elongation is delayed and not completely impaired. The defects observed in crb mutant cells support the hypothesis that Crb is a crucial regulator of the interconnection between the actomyosin cytoskeleton and AJs (Salis, 2017).

The fragmentation of AJs upon Crb depletion has been already described, for example in embryo or during follicular morphogenesis. However, in these two systems the function of Crb has been related to the role of Moe in the regulation of the actomyosin cytoskeleton, while the role of Yurt has never been addressed or has been excluded. The current data support that in pupal wing cells the role of Crb in the stability of the AJs is likely established via Yurt. Crb is shown to modulate Yurt localization at the SAR at the end of hexagonal packing and yurt mutant cells phenocopy crb mutant cortical defects. Nonetheless, previous studies in cultured cells have established that Yurt participates in epithelial polarity and organization of apical membranes by negatively regulating the activity of the Crb complex. On the contrary, this study shows that, whereas Crb modulates Yurt distribution at the SAR at the end of hexagonal packing of wing cells, Yurt depletion does not impact Crb association to the SAR, with the exception of the E-cad- and F-actin-devoid gaps. Yurt and Crb similarly act on actomyosin and E-cad organization at the cell-cell junctions suggesting that the coordinated function of these two proteins is regulated by different mechanisms in different tissues. On the other hand, moe depletion does not specifically modify Crb distribution at the SAR, a finding coherent with the evidence that Moe is not implicated in stability of AJs in this tissue, as opposed to other models (Salis, 2017).

Studies based on in vivo mechanical measurements or mathematical/physical modeling have proposed that epithelial cell packing results from a balance between intrinsic cell tension and extrinsic tissue-wide forces to establish a correct and robust order in the tissue. Hence, the tension generated by the actomyosin cortex and the pressure transmitted through adherens junctions are the two main self-organizing forces driving tissue morphogenesis. Tension shortens cell-cell contacts and pressure of individual cells counteracts tension to maintain cell size. The current data indicate that Crb recruits at SAR Moe and Yurt, which show opposite effects on pupal wing morphogenesis. While Moe promotes cell expansion, Yurt controls cell constriction and the stability of the AJs and of the actomyosin network. In crb mutant cells, the absence of variation in the cell perimeter might be explained by the simultaneous loss of positive and negative regulators. Therefore, Crb acts as a coordinator of the two self-organizing mechanisms implicated in morphogenesis. Additionally, the dynamic redistribution of Crb at the SAR at the end of hexagonal packing, together with the disruption of cell orientation in crb mutants, is consistent with the hypothesis that Crb is required to stabilize cell shape and pattern in order to properly progress throughout tissue development (Salis, 2017).

In conclusion, these functional analyses during pupal wing morphogenesis allowed the unraveling Crb-dependent mechanisms that are integrated to produce shape changes during development independently of epithelial polarity. Furthermore, the results show that the interplay between Crb and FERM proteins is tissue-regulated and that their epistatic interactions differ in a spatio-temporal manner (Salis, 2017).


cDNA clone length - 2418

Bases in 5' UTR - 247

Exons - 9

Bases in 3' UTR - 343


Amino Acids - 575

Structural Domains

Moesin shares 58% overall identity with both human moesin and human ezrin, 57% identity with mouse radixin, and only 41% identity with human merlin. Designation of this gene as Moesin, rather than as ezrin or radixin, is based primarily on its lack of the polyproline tract characteristic of the latter two proteins. furthermore, Moesin shares a greater degree of identity in the C-terminal divergent region with human moesin (26%) than with human ezrin (25%) or mouse radixin (22%) (McCartney, 1996).

Analysis of the Drosophila genome identified a single gene encoding an ERM protein, which had been previously cloned and named Moesin. However, when compared with human ERMs, Drosophila Moesin has strongest conservation with Radixin in the FERM domain, with Ezrin in the C-terminal tail, and shares with mammalian Moesin the absence of a polyproline track that is found in both Ezrin and Radixin. To gain further insight into the evolution of ERMs, sequence conservation of ERM proteins was analyzed from various species. Each available ERM protein from vertebrates seems to be a true orthologue of Ezrin, Radixin or Moesin, respectively, suggesting that three ERM genes exist in these species. By contrast, in common with Drosophila, ERMs from Caenorhabditis elegans and the snail Biomphalaria glabrata are equally related to Ezrin, Radixin and Moesin, thus suggesting that the presence of three ERM genes is restricted to deuterostomes. However, Merlin/NF2 proteins from protostomes and deuterostomes are more related to each other than to ERM, indicating that divergence between NF2 and ERM preceded the separation of these two taxa. Within the C-terminal tail, Drosophila Moesin also contains residues characteristic of Ezrin, Radixin or Moesin, and the threonine involved in ERM activation is conserved in Drosophila Moesin (position 559). Thus, Drosophila Moesin seems to be a prototypical ERM protein, sharing both common and specific features with all three vertebrate ERMs (Polesello, 2002).

Moesin : Evolutionary Homologs | Regulation | Developmental Biology | Effects of Mutation | References

date revised: 25 October 2004

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