Cyclin-dependent kinase subunit 30A: Biological Overview | References
Gene name - Cyclin-dependent kinase subunit 30A
Cytological map position-30A8-30A8
Function - signaling
Symbol - Cks30A
FlyBase ID: FBgn0010314
Genetic map position - 2L:9,330,767..9,331,981 [+]
Classification - CKS, Cyclin-dependent kinase regulatory subunit
Cellular location - not specified in literature
Cks is a small highly conserved protein that plays an important role in cell cycle control in different eukaryotes. Cks proteins have been implicated in entry into and exit from mitosis, by promoting Cyclin-dependent kinase (Cdk) activity on mitotic substrates. In yeast, Cks can promote exit from mitosis by transcriptional regulation of cell cycle regulators. Cks proteins have also been found to promote S-phase via an interaction with the SCFSkp2 Ubiquitination complex. The Drosophila Cks gene, Cks30A (corresponding to the gene remnants), is required for progression through female meiosis and the mitotic divisions of the early embryo through an interaction with Cdk1 (Cdc2). Cks30A mutants are compromised for Cyclin A destruction, resulting in an arrest or delay at the metaphase/anaphase transition, both in female meiosis and in the early syncytial embryo. Cks30A appears to regulate Cyclin A levels through the activity of a female germline-specific anaphase-promoting complex, CDC20-Cortex. A second closely related Cks gene, Cks85A, plays a distinct, non-overlapping role in Drosophila, and the two genes cannot functionally replace each other (Swan, 2005).
Passage through the cell cycle must be precisely regulated during development. A number of conserved cell cycle regulators have been shown to act at important developmental transitions in various organisms, including Drosophila. Cks, or Suc1, is a small cell cycle regulator that was first identified by its ability to interact genetically and physically with the Cyclin-dependent kinases (Cdks) in yeasts (Hadwiger, 1989; Hayles, 1986). Members of this family were subsequently found in many multicellular organisms, including Xenopus, Caenorhabditis elegans and humans (reviewed by Bartek, 2001; Pines, 1996). In addition to interacting with Cdks, Cks proteins share an anion-binding domain implicated in binding to the phospho-epitope Ser/pSer/pThr/X, found on many mitotic proteins; and a domain that mediates a conformational switch between a monomeric and a dimeric form in vitro (Swan, 2005 and references therein).
Despite the strong conservation of these proteins, studies in different animal models have revealed a surprising number of distinct roles at different points in the cell cycle. Studies of the Cks2 homolog in Xenopus (Xe-p9) indicate a role in the G2-M transition and the metaphase/anaphase transition, possibly by linking the Cdk to its substrates (Patra, 1996; Patra, 1998; Patra, 1999; Spruck, 2003). Mammalian Cks2 and C. elegans CKS-1 are also required for the metaphase/anaphase transition (Polinko, 2000; Spruck, 2003). Cks1 in mammals plays a seemingly unrelated role in promoting S-phase progression as an adaptor protein that links the SCFSkp2 complex to one of its substrates, the Cdk inhibitor p27 (Ganoth, 2001; Spruck, 2001). Recent work in yeast has revealed yet another function for Cks. Saccharomyces cerevisiae Cks1 was found to recruit the 19S and 20S proteosome to the promoter of the CDC20 gene to promote its transcription late in mitosis (Morris, 2003). In metazoans, which all appear to have two Cks genes, it is not yet clear how the two Cks genes carry out these diverse roles, and to what degree there is functional redundancy between them (Swan, 2005 and references therein).
Female meiosis in Drosophila represents an excellent system for studying the developmental control of cell cycle progression. Female meiosis progresses through well-characterized transitions that are linked to development of the oocyte. With the aim of identifying genes required for completion of female meiosis in Drosophila, this study analyzed a collection of maternal effect mutants that arrest early in embryogenesis. It was found that the remnants (rem) gene is required for female meiosis and for mitosis in the early embryo, and that this gene corresponds to the Drosophila Cks gene at cytological location 30A (Cks30A). In addition to its maternal role, Cks30A appears to function with Cdk1 to prevent S-phase in G2-arrested histoblasts in the larva. Cks30A interacts with Cdk1/cyclin complexes, and this interaction is necessary for its function. One of the key functions of Cks30A is to mediate the destruction of Cyclin A, and evidence is presented that this is through an effect on the activity of Cortex, a germline-specific adaptor for the Anaphase-Promoting Complex (APC). It was also found that a closely related Cks gene, Cks85A, plays a distinct role in Drosophila, and the two genes cannot substitute for each other in vivo (Swan, 2005).
The maternal effect lethal gene, remnants, corresponds to one of the two Drosophila Cks genes (Cks30A). Analysis of two hypomorphic alleles and a null allele made by homologous recombination confirmed that Cks30A is not essential for cell cycle regulation in most tissue types. Rather, Cks30A functions in specialized cell cycles: the abdominal histoblast divisions, female meiosis and the syncytial divisions of the early embryo (Swan, 2005).
Cks30A mutants displayed a strikingly simple mitotic phenotype: most embryos from mutant females arrested in metaphase of the first mitotic division. Similarly, Cks30A mutants display a pronounced delay or arrest in metaphase of female meiosis II. Therefore, there is a common requirement for Cks30A in metaphase to anaphase progression in female meiosis II and in early embryonic mitosis. The second meiotic division is similar to a mitotic division in that it involves the segregation of sister chromatids, and therefore Cks30A may be part of a conserved machinery that is required for both of these processes (Swan, 2005).
An alternative explanation for the mitotic arrest in Cks30A mutants is that it is a secondary effect of a prior failure in meiosis or pronuclear fusion. However, FISH experiments indicate that this mitotic arrest occurs even in embryos that successfully undergo pronuclear fusion. Also, mutations in alpha-Tubulin67C, that block pronuclear fusion, do not lead to a mitotic arrest in the embryo, indicating that these events are not coupled (Swan, 2005).
In addition to a crucial role in exit from mitosis, Cks30A is important in at least one aspect of entry into mitosis: spindle formation. Cks30AKO mutants are severely delayed in assembly of the first mitotic spindle. Cks30A was also required for proper assembly of the female meiotic spindle and the specialized spindle-like microtubule aster of the polar bodies. Therefore, Cks30A appears to be required at two points in the mitotic (or meiotic) cell cycle: in prometaphase for spindle assembly and at the metaphase-to-anaphase transition (Swan, 2005).
These dual roles for Cks30A in meiosis appear to be at least partially conserved in other metazoans. Xenopus Cks2 (Xe-p9), like Cks30A, is required for the metaphase-to-anaphase transition in meiosis II. Xenopus Cks2 is also required in vitro for entry into mitosis, and this may be related to the in vivo requirement for Cks30A in spindle assembly in meiosis and mitosis. Cks genes in other eukaryotes also appear to have related but distinct functions in meiosis. Mouse cks2 is essential for anaphase progression in meiosis I of both male and female meiosis, while in C. elegans cks-1 is not required for entry into anaphase, but is necessary for proper chromosome segregation in meiosis I, possibly reflecting a role in meiotic spindle assembly. Therefore, Cks genes appear to share a common requirement in entry into and exit from meiosis in different eukaryotes (Swan, 2005).
The two roles for Cks30A appear to reflect a conserved role in promoting Cdk1 activity. Cdk1 is the central mitotic Cdk, and its kinase activity on specific mitotic proteins is required for entry into mitosis, including spindle assembly, and in maintaining the metaphase state. Cdk1 activity is also required for exit from mitosis through activation of the APC, which in turn promotes anaphase progression by targeting mitotic cyclins for destruction. In Drosophila Cks30A and Cdk1 interact in vivo, and mutations in Cks30A that disrupt Cdk1 binding are compromised for activity in vivo. Cks30A also interacts genetically with Cdk1 in another cell type, the abdominal histoblasts. Therefore, genetic and physical evidence supports the conclusion that the observed interaction with Cdk1 is required for Cks30A function (Swan, 2005).
Cks30A is required for the destruction of Cyclin A in the ovary and in the syncytial embryo and two observations argue that it is this failure to degrade Cyclin A that results in the observed delay or arrest in metaphase of meiosis II and in mitosis: (1) a similar metaphase arrest is seen in cellularized embryos expressing non-degradable Cyclin A, while syncytial embryos with a slight excess of Cyclin A (approximately 1-3 x wild type) due to mutations in grapes display a metaphase delay; (2) the Cks30A mutant phenotype can be partially rescued by lowering Cyclin A levels. The CDC20 homolog, cortex, is also required for exit from meiosis II, and cortex is also required for Cyclin A destruction in the ovary. These results argue that Cks30A and the APCCortex function in the same pathway leading to Cyclin A destruction, although the possibility that Cks30A and Cortex act in independent pathways to promote Cyclin A destruction cannot be ruled out (Swan, 2005).
In vitro studies of Cks2 in Xenopus have led to a model in which Cks bound to Cdk1 recruits phosphorylated Cdk1 substrates to the kinase, allowing these substrates to be more efficiently recognized and thereby further phosphorylated by Cdk1. The CDC27 and CDC16 components of the APC are key targets of Cks-Cdk1 phosphorylation in Xenopus. While it is not yet clear how APC phosphorylation leads to its activation, there is evidence that one of the effects of phosphorylation is to stimulate CDC20 binding to the APC. Therefore it is possible that in Drosophila Cks30A-Cdk1 phosphorylates the APC, and this phosphorylation specifically stimulates the association of Cortex with the APC. Alternatively, Cks30A-Cdk1 may directly phosphorylate and activate Cortex (Swan, 2005).
In mammalian cells and in the cellularized embryo, the completion of mitosis depends on the sequential destruction of the three mitotic cyclins by the APCFzy. Cyclin A is destroyed first in prometaphase, dependent on APCFzy activity. Although the APCFzy is active, the spindle checkpoint is thought to inhibit its activity on Cyclin B. Upon spindle assembly, the checkpoint is relieved and APCFzy can mediate Cyclin B destruction (Swan, 2005).
It now appears that some but not all aspects of anaphase progression are conserved in the second meiotic division and in the nuclear divisions of the syncytial embryo. Although overall Cyclin B levels do not oscillate during the early syncytial divisions (cycles 1 to 8), Cyclin B appears to undergo local degradation on the mitotic spindle at anaphase, and the injection of stabilized Cyclin B into syncytial embryos results in an early anaphase arrest. By contrast to the early anaphase arrest upon Cyclin B stabilization, the injection of an APC-inhibiting peptide into early embryos results in a metaphase arrest. The results suggest that this metaphase arrest is due to the failure of APCCortex-mediated Cyclin A destruction. Like Cyclin B levels, Cyclin A levels do not oscillate detectably in cycles 1 to 7, although unlike Cyclin B, this appears to be due to a balance between constant destruction and new protein synthesis. Despite this difference, it remains possible that local oscillations in Cyclin A and Cyclin B could drive these syncytial cell cycles (Swan, 2005).
While the importance of cyclin destruction may be conserved in the early embryo, the means by which the cyclins are destroyed appears to be different. In cellularized embryos, the APCFzy is responsible for the sequential destruction of all three mitotic cyclins. In the syncytial embryo, Fzy is not required for Cyclin A destruction. Cortex, a diverged, female germline-specific CDC20, targets Cyclin A for destruction, but has no detectable effect on Cyclin B or B3 levels in the syncytial embryo. It remains possible that Cortex is responsible for the destruction of local pools of maternal Cyclin B (and possibly B3). Alternatively, the known maternal requirement for fzy may reflect a role in the local destruction of these cyclins. This would suggest a model in which the germline utilizes two CDC20 homologs, Cortex and Fzy, to mediate the sequential destruction of Cyclins A, B and possibly B3 in the syncytial embryo. Further work will be needed to test this model. It is also not clear if Cyclin A is the only target of the APCCortex and if the APCCortex is the only target of Cks30A-Cdk1. In addition to metaphase arrest, Cks30A mutants have spindle assembly delays or defects, a phenotype that has not been observed in other cell types to result from a failure to degrade Cyclin A. Interestingly, cortex mutants also have abnormal meiosis II spindles and fail to assemble a mitotic spindle around the male pronucleus, suggesting the possibility that the sole function of Cks30A in the female germline is to activate Cortex. Like Cks30A, C. elegans cks-1 and mouse cks2 appear to be predominantly required for meiosis, and this may also reflect specific roles in activating meiosis-specific APC complexes. The histoblast requirement for Cks30A, in contrast, is unlikely to represent a role in Cortex activation (or subsequent Cyclin A destruction), since cortex mutants, either alone or in combination with Cks30A, have no effect on abdominal development (Swan, 2005).
A specific requirement for Cks30A in activation of the maternal-specific APCCortex would explain why Cks30A is essential for anaphase progression in female meiosis and the syncytial embryo but not in most cell types. An alternative possibility, that Cks30A is functionally redundant with the other Drosophila Cks, Cks85A, cannot be ruled out. Cks85A mutants alone or in combination with Cks30A, do not have obvious defects in exit from mitosis. Furthermore, while closely related to Cks30A, Cks85A cannot replace Cks30A when expressed in the female germline, and Cks30A cannot replace Cks85A when expressed zygotically. Therefore, it is concluded that the two Drosophila Cks genes have distinct and non-overlapping functions. Recently, Cks85A was found to interact with a Drosophila Skp2 homolog in a genome-wide yeast two-hybrid screen. Two residues on Cks1 have recently been found to be crucial for Skp2 binding in vitro, and these residues are conserved or similar in Drosophila Cks85A. If indeed Cks85A represents the Drosophila Cks1 ortholog, it is perhaps not surprising that Cks30A cannot functionally replace Cks85A, since it has been found that Cks2 orthologs cannot bind Skp2 in vitro. However, it is unexpected that Cks85A cannot substitute for Cks30A in vivo. To date all Cks proteins tested can stimulate the Cdk-dependent phosphorylation of mitotic proteins in vitro, and the mouse Cks1 can functionally replace Cks2 in vivo. The failure to rescue Cks30A mutant phenotypes cannot be due to an inability of Cks85A to interact with Cdks, since Cks85A binds Cdks with even greater affinity than does Cks30A. It is possible that, analogous to the Cks1/Skp2 interaction, Cks30A has an as-yet-to-be-identified partner that is necessary for its mitotic activities. Cks85A would, therefore, be unable to carry out the mitotic activities because of an inability to bind this putative Cks30A partner (Swan, 2005).
In conclusion, Drosophila Cks30A is crucial for Cdk1 activity in spindle assembly and anaphase progression in female meiosis and early embryonic mitosis, and at least part of this activity appears to be to regulate Cyclin A levels. Cks30A functions non-redundantly with another closely related Drosophila cks, Cks85A (Swan, 2005).
Meiosis is a highly specialized cell division that requires significant reorganization of the canonical cell-cycle machinery and the use of meiosis-specific cell-cycle regulators. The anaphase-promoting complex (APC, a machine for degrading proteins; see APC subunits Cdc27 and morula; for review see Acquaviva, 2006) and a conserved APC adaptor/activator, Cdc20 (also known as Fizzy), are required for anaphase progression in mitotic cells. The APC has also been implicated in meiosis, although it is not yet understood how it mediates these non-canonical divisions. Cortex (Cort) is a diverged Fzy homologue that is expressed in the female germline of Drosophila, where it functions with the Cdk1-interacting protein Cks30A to drive anaphase in meiosis II. This study shows that Cort functions together with the canonical mitotic APC adaptor Fzy to target the three mitotic cyclins (A, B and B3) for destruction in the egg and drive anaphase progression in both meiotic divisions. In addition to controlling cyclin destruction globally in the egg, Cort and Fzy appear to both be required for the local destruction of cyclin B on spindles. Cyclin B associates with spindle microtubules throughout meiosis I and meiosis II, and dissociates from the meiotic spindle in anaphase II. Fzy and Cort are required for this loss of cyclin B from the meiotic spindle. These results lead to a model in which the germline-specific APCCort cooperates with the more general APCFzy, both locally on the meiotic spindle and globally in the egg cytoplasm, to target cyclins for destruction and drive progression through the two meiotic divisions (Swan, 2007).
Cks30A belongs to a highly conserved family of proteins that bind to and stimulate the activity of the mitotic kinase Cdk1. In Xenopus, the Cks30A homologue Xep9 stimulates the Cdk-dependent phosphorylation of APC subunits, and thereby promotes the activation of the APCFzy complex (Patra, 1998). The current results suggest that Cks30A may have a similar role in stimulating both the APCFzy and APCCort in female meiosis in Drosophila. (1) Cks30A, like cort and fzy, is required for the completion of meiosis II and, like fzy, it is required for the completion of the first mitotic division of embryogenesis. (2) Cks30A, as are Cort and Fzy, is necessary for global cyclin destruction in the Drosophila egg and for local cyclin B destruction on the meiotic spindle. Global levels of cyclin A and cyclin B3 are elevated to a greater extent in Cks30A mutants than in single mutants for cort or fzy, consistent with the idea of Cks30A activating both Cort and Fzy. (3) Cks30A is necessary for the activity of ectopically expressed Cort in the adult wing. Cks30A may also play a role in activating APCFzy in mitotic cells. the temperature-sensitive fzy6 allele is lethal at all temperatures in a Cks30A-null background, suggesting that the Cks30A-dependent activation of APCFzy becomes essential when Fzy activity is compromised (Swan, 2007).
Although Cks30A appears to promote the activity of the APCCort and the APCFzy, these complexes seems to retain some activity in the absence of Cks30A. Whereas cort and fzy cause an arrest in meiosis II, Cks30A-null mutants are typically delayed only in meiosis II (Swan, 2005). Also, although cyclin A and cyclin B3 levels are elevated more in Cks30A eggs than in either fzy or cort, their levels are still not as high as in fzy; cort double mutants, indicating that Fzy and Cort can destroy cyclin A and cyclin B3 to some degree in the absence of Cks30A. Cyclin B destruction is even less dependent on Cks30A, because cyclin B levels are affected less in Cks30A mutants than in either cort or fzy single mutants. Therefore, Cks30A may be more crucial for the activity of APCCort and APCFzy complexes on cyclin A and cyclin B3, and less crucial for their activity on cyclin B. The relatively weaker meiotic arrest in Cks30A mutants compared to fzy; cort double mutants may also indicate that the APC has other meiotic targets that can be destroyed in the absence of Cks30A (Swan, 2007).
Conventional centrosomes are absent from a female meiotic spindle in many animals. Instead, chromosomes drive spindle assembly, but the molecular mechanism of this acentrosomal spindle formation is not well understood. This study screened female sterile mutations for defects in acentrosomal spindle formation in Drosophila female meiosis. One of them, remnants (rem), disrupted bipolar spindle morphology and chromosome alignment in non-activated oocytes. It was found that rem encodes a conserved subunit of Cdc2 (Cks30A). Since Drosophila oocytes arrest in metaphase I, the defect represents a new Cks function before metaphase-anaphase transition. In addition, it was found that the essential pole components, Msps and D-TACC, are often mislocalized to the equator, which may explain part of the spindle defect. The second cks gene cks85A, in contrast, has an important role in mitosis. In conclusion, this study describes a new pre-anaphase role for a Cks in acentrosomal meiotic spindle formation (Pearson, 2005).
Spindle formation in female meiosis is unique in terms of the absence of conventional centrosomes. Instead, chromosomes have a central role in the assembly of spindle microtubules. This acentrosomal (also called acentriolar or anastral) spindle formation is common in female meiosis for many animals including mammals, insects and worms. Despite potential medical implications, this spindle formation is much less studied than centrosome-mediated spindle formation in mitosis (Pearson, 2005).
Drosophila provides a valuable tool to study the acentrosomal spindle formation in vivo. Unlike many other species, mature non-activated Drosophila oocytes arrest in metaphase of meiosis I until ovulation, which coincides with fertilization. This provides a unique opportunity to study spindle formation, without interference from chromosome segregation or meiotic exit (Pearson, 2005).
Two components of acentrosomal spindle poles, Msps and D-TACC, physically interact and are crucial for spindle bipolarity (Cullen, 2001). Other studies have identified essential components for spindle formation, such as kinesin-like proteins (Ncd and Sub, γ-tubulin, and a membrane protein surrounding the spindle (Axs). Some of these spindle components are probably modulated by cell-cycle regulators, but knowledge of the regulation is limited. To identify essential components and regulators, a cytological screen was performed for mutants defective in acentrosomal spindle formation of non-activated oocytes (Pearson, 2005).
Through the screen, remnants was identified and identified as a mutant of a Drosophila Cks/Suc1 homologue, Cks30A. Cks is the third subunit of the Cdc2 (Cdk1)-cyclin B complex, but the role of Cks is less clearcut than that of other subunits of the complex. It is implicated in entry into mitosis/meiosis, metaphase-anaphase transition, exit from mitosis/meiosis and inactivation of Cdk inhibitors (Patra, 1996
For molecular analysis of the acentrosomal spindle in Drosophila female meiosis, female sterile mutants were screened for spindle defects in non-activated oocytes. Female sterile mutants on the second chromosome have previously been isolated. This study focused on classes of mutants that lay eggs that do not develop beyond the blastoderm stage. This category of mutants includes known meiotic mutants affecting spindle formation, such as fs(2)TW1 (γ-tubulin 37C) and subito (a kinesin-like protein (Pearson, 2005).
The identity of the remnants (rem) gene was identified by positional cloneing. The rem gene was previously mapped to 30A-C using a deficiency Df(2L)30AC (Schupbach, 1989). One missense mutation was identified in the gene CG3738 (cks, hereafter called cks30A; Finley, 1994). There were no other mutations within coding sequences and splicing junctions in the region. In addition, the amount and size of the transcripts that are known to be expressed in adult females was tested, and no differences were found between rem and wild type (Pearson, 2005).
Cks30A is one of two Drosophila homologues of Saccharomyces cerevisiae Cks1/Schizosaccharomyces pombe Suc1, a conserved subunit of the Cdc2 (Cdk1)/cyclin B complex, and has been shown to interact with Cdc2 (Finley, 1994). The mutation in rem1 results in a conversion of the 61st amino acid from proline to leucine. This proline is completely conserved among all Cks homologues, further confirming that the mutation is not a polymorphism. Crystal structure analysis has indicated that this residue forms part of the interaction surface with Cdc2 (Bourne, 1996). Immunoblots using an anti-human Cks1 antibody indicated that this mutation disrupts the stability of the Cks30A protein (Pearson, 2005).
To explain the role of Cks30A, focus was placed on the rem1 mutant in non-activated oocytes, which arrest in metaphase I. Non-activated oocytes were dissected from wild type and the rem1 mutant, and chromosomes and spindles were visualized by immunostaining (Pearson, 2005).
In wild type, non-activated mature oocytes contain a single bipolar spindle around chromosomes. Bivalent chromosomes align symmetrically with chiasmatic chromosomes at the equator and achiasmatic chromosomes that are located nearer the poles. The rem1 mutant was able to enter meiosis, condense chromosomes and assemble microtubules around chromosomes. However, only a minority of spindles showed normal spindle morphology and chromosome alignment (Pearson, 2005).
The most prominent defect in the rem1 mutant was chromosome misalignment. This defect was observed in about a half of the spindles. Even in the cases in which the spindle remained well organized, chiasmatic chromosomes often moved away from the equator and lost overall symmetrical distribution. The second class of defect in the rem1 mutant was abnormal spindle morphology. Although the abnormality varied from spindle to spindle in the rem1 mutant, the most typical defect was the formation of ectopic poles near the spindle equator. The focusing of spindle poles seemed to be unaffected (Pearson, 2005).
Further quantitative analysis showed no significant difference between the phenotypes of rem1 homozygotes (rem1/rem1) and hemizygotes (rem1/Df). This indicates that the rem1 mutation is genetically amorphic. A recent independent study (Swan, 2005) has indicated that another weaker allele remHG24 (Schupbach, 1989) shows similar abnormalities at a lower frequency. These results indicate that Cks30A is required before the metaphase-anaphase transition for spindle morphology and chromosome alignment (Pearson, 2005).
To gain an insight into the spindle defects in female meiosis, the localization of Msps was examined. Msps protein belongs to a conserved family of microtubule regulators, including XMAP215, and is the first protein identified at the acentrosomal poles in Drosophila (Cullen, 2001; Ohkura, 2001). An msps mutation often leads to the formation of a tripolar spindle in female meiosis I (Pearson, 2005).
In wild type, Msps protein is accumulated at the acentrosomal poles of the metaphase I spindle in female meiosis, although the localization sometimes spreads to the spindle microtubules. In the rem1 mutant, although the Msps protein is still concentrated at the poles, it is often accumulated around the equator of the spindle. Mislocalization of this important pole protein to the equator in the rem1 mutant may sometimes lead to the formation of ectopic spindle poles near the equator (Pearson, 2005).
Msps localization is dependent on another pole protein D-TACC, which binds to Msps (Cullen, 2001). To test whether D-TACC also mislocalizes, the localization of D-TACC was examined in the rem1 mutant. In wild type, D-TACC is highly concentrated at the acentrosomal pole. In the rem1 mutant, D-TACC often accumulates at the spindle equator, although it is still concentrated around the poles to some degree. In summary, Cks30A is required for correct localization of the essential pole proteins, Msps and D-TACC (Pearson, 2005).
To gain an insight into how the defect in the Cdc2 complex leads to Msps or D-TACC mislocalization to the spindle equator, the localization of cyclin B was examined. Cyclin B is considered to be the main determinant of the activity and cellular localization of the Cdc2 complex. Immunostaining in non-activated oocytes showed that cyclin B is localized to the metaphase I spindle, with a concentration around the spindle equator. This cyclin B localization could suggest a possible regulatory role of the Cdc2 complex in the transport of Msps and D-TACC from the spindle equator to the poles. The cyclin B localization is not affected in the rem mutant, suggesting that Cks30A mainly affects the substrate specificity of the Cdc2 complex, as shown in other systems (Patra, 1998; Pearson, 2005).
The Drosophila genome contains one more predicted cks homologue (CG9790), which is called cks85A. Although mammalian genomes also have two Cks genes, they are more similar in sequence to each other than to either of the two cks genes in Drosophila (Pearson, 2005).
The gene expression pattern of the two cks genes was examined during Drosophila development. RNAs were isolated from various stages of development and analysed by reverse transcription-PCR (RT-PCR) using primers that correspond to each of the cks genes. cks30A gave strong signals in adult females and embryos, whereas it gave only weak signals in adult males, larvae and pupae. This maternal expression pattern is consistent with the observed female sterile phenotype of the cks30A (rem1) mutant. In contrast, cks85A signals were obtained more uniformly throughout the development without sex specificity in adults. In S2 cultured cells, which originated from embryos, both genes were well expressed (Pearson, 2005).
To identify the Cks proteins, an anti-human Cks1 antibody was used for immunoblots of protein extracts from embryos and S2 cells. Although the antibody recognized many proteins, two bands were detected within a range of molecular weights consistent with the Cks proteins. In embryos laid by the rem1 mutant, the amount of the smaller band was greatly reduced. To further confirm their identity, S2 cells were subjected to RNA interference (RNAi) using doublestranded RNAs (dsRNAs) corresponding to the cks genes. It was found that both of the bands disappeared when both genes were simultaneously knocked down by RNAi. It indicated that, consistent with RT-PCR results, S2 cells produced both the Cks proteins and that RNAi effectively depletes them (Pearson, 2005).
Cytological analysis showed that cks85A RNAi results in a significant increase in chromosome misalignment/missegregation and spindle abnormality in mitosis after an extended time, whereas cks30A RNAi has a lesser impact on mitotic progression. About a half of anaphase or telophase cells had lagging chromosomes or chromosome bridges after cks85A RNAi. In some cases, spindles contained scattered chromosomes the sister chromatids of which were either attached or detached. The frequency of multipolar spindles was also increased. The genetic and RNAi results indicated that cks85A has an important function in mitotic progression, whereas cks30A mainly functions in female meiosis (Pearson, 2005).
This study has shown a new pre-anaphase function of a Cks protein in acentrosomal spindle formation during Drosophila female meiosis. Through a cytological screen, spindle defects in remnants among female sterile mutants. Cytological analysis showed that Cks30A is required for correct formation of the acentrosomal spindle and chromosome alignment in female meiosis I. The observation on mislocalization of the essential pole components, Msps and D-TACC, in the mutant provides a molecular insight into a role of Cks30A in spindle morphogenesis (Pearson, 2005).
Cks/Suc1 protein is the third subunit of the Cdc2-cyclin B complex, which is conserved across eukaryotes. Although it has been known to be essential for the cell cycle, the function seems to be less straightforward than that of the other subunits of the Cdc2 complex. One reason is that Cks also interacts with other Cdks and has Cdk-independent functions (Ganoth, 2001; Spruck, 2001). Even if Cks is limited to roles in mitosis/meiosis, Cks proteins are implicated in entry into mitosis/meiosis, metaphase-anaphase transition and also exit from mitosis/meiosis (Patra, 1996; Polinko, 2000; Spruck, 2003). Furthermore, the roles of Cks were further complicated by the fact that animal genomes encode two Cks homologues (Pearson, 2005).
Studies in Caenorhabditis elegans and mice showed that one of two cks genes is required for female fertility (Polinko, 2000; Spruck, 2003). Similarly, the results indicated that one of two Drosophila cks homologues, cks30A, is expressed maternally and is required for female meiosis. Further analysis indicated that Cks30A is required for proper bipolar spindle formation and chromosome alignment in mature oocytes arrested in metaphase I. In C. elegans, depletion of one of the Cks proteins by RNAi results in a failure to complete meiosis I (Polinko, 2000). Similarly, in mice, oocytes from a Cks2 knockout cannot progress past metaphase I and a small percentage of oocytes show chromosome congression failure (Spruck, 2003). In both cases, the defects were interpreted mainly as post-metaphase defects. Since Drosophila non-activated oocytes are arrested in metaphase I until ovulation, pre-anaphase function of Cks30A can be distinguised from possible post-metaphase function. This study clearly showed that Drosophila Cks30A has a function in establishing metaphase I, in addition to later functions that have reported recently (Swan, 2005; Pearson, 2005).
At the moment, it is not known how the cks30A mutation disrupts spindle formation and chromosome alignment in female meiosis. It has been thought that a loss of Cks function affects the Cdc2 activity towards certain substrates. It was found that the essential pole components, Msps and D-TACC, mislocalize to the spindle equator in the mutant. Previously, it was hypothesized that Msps is transported by the Ncd motor and anchored to the poles by D-TACC (Cullen, 2001). D-TACC localizes to the poles independently from Ncd, but may also be transported from the spindle equator along microtubules by other motors. Cks30A-dependent Cdc2 activity may be required for activating the transport system at the onset of spindle formation in female meiosis. Consistently, it was found that cyclin B is concentrated around the equator of the metaphase I spindle. Msps is the XMAP215 homologue and belongs to a family of conserved microtubule-associated proteins (Cullen, 1999; Ohkura, 2001). It is a major microtubule regulator, both in mitosis/meiosis and interphase. The mislocalization of this microtubule-regulating activity could lead to the disruption of spindle organization in the mutant (Pearson, 2005). Cks85A and Skp2 interact to maintain diploidy and promote growth in Drosophila
The Cks or Suc1 proteins are highly conserved small proteins that play remarkably diverse roles in the cell cycle. All Cks homologues have the ability to associate with Cyclin dependent kinases (Cdks) and in many cases this interaction has been shown to be important for function. This study characterized the null and RNAi knockdown phenotype of the Drosophila Cks1 (Cks85A) gene. Cks85A is essential for viability in Drosophila. Cks85A null animals have reduced overall growth and this correlates with reduced ploidy and impaired DNA replication in endoreplicating cells. Interestingly, Cks85A is also required for the maintenance of diploidy in mitotically cycling cells. The requirement for Cks85A in growth is similar to that of the mammalian Cks1, which was found to interact with the SCFSkp2 ubiquitin ligase. The Drosophila Skp2 gene was identifed and generated null alleles were generated. Comparison of these mutants to null mutants for Cks85A reveals a remarkably similar dual requirement in growth and in maintenance of diploidy. Cks85A interacts directly with the SCFSkp2 ubiquitin ligase and genetic evidence indicates that this is its major molecular function. The closely related Cks30A cannot interact with the SCFSkp2 and cannot functionally compensate for loss of Cks85A. It was also found that the critical growth promoting and diploidy maintaining functions of Cks85A and Skp2 are independent of known SCFSkp2 substrates, p27 and Cdt1, indicating that other critical substrates remain to be identified (Ghorbani, 2011).
The small Cdk subunit, Cks or suc1 was first identified on the basis of a genetic and physical interaction with the Cyclin dependent kinase (Cdk) in fission yeast and budding yeast. In addition to a completely conserved Cdk interacting region, all Cks proteins have an anion binding domain that appears to allow interaction with phosphoepitopes. All metazoans studied to date have two Cks proteins (Cks1 and Cks2 in mammals) that appear to have both distinct and redundant functions. Xenopus Xe-p9, mammalian Cks2 and Drosophila Cks30A are necessary for meiosis, and are necessary for both entry into and exit from meiosis. The anaphase role appears to involve the recruitment of cyclin-Cdk complexes to the Anaphase Promoting Complex (APC) to promote either or both APC phosphorylation by the Cdk or cyclin ubiquitination by the APC. In vitro studies in the murine model indicate that in addition to this meiotic role, Cks2 functions redundantly with Cks1 in the entry into mitosis. Surprisingly, this appears to reflect a role in transcription -- specifically of mitotic cyclins and Cdk1 (Martinsson-Ahlzen, 2008; Ghorbani, 2011 and references therein).
Studies of mammalian Cks1 revealed yet another novel role for Cks proteins, as part of the SCFSkp2 ubiquitin ligase. SCFSkp2 is important for the ubiquitination and proteasome-mediated destruction of a number of proteins, many of which have key roles in S-phase regulation (Nakayama, 2006). Cks1 interacts with Skp2 and is required for the recognition of a critical SCFSkp2 substrate, the Cdk inhibitor p27. Cks1 knockout in the mouse results in reduced growth and elevated p27 levels, and phenotypes are also observed upon Skp2 knockout (Ghorbani, 2011).
While in vitro studies and the shared phenotypes suggest a close functional relationship between Cks1 and Skp2, it remains unclear to what degree Cks1 is necessary for the targeting of SCFSkp2 substrates other than p27. It is also not known to what degree Cks1 functions independent of SCFSkp2 and to what extent it is redundant with its close homologue, Cks2 (Ghorbani, 2011).
This study presents the characterization of the Drosophila Cks85A gene. Cks85A was found to be required for growth and for the maintenance of diploidy. Cks85A is the Drosophila Cks1 orthologue, and interacts with Skp2 as part of the SCFSkp2 complex. Using genetic and biochemical approaches, the relationships in vivo between Cks85A and Skp2, and between Cks85A and the other Drosophila Cks protein, Cks30A, were determined (Ghorbani, 2011).
Cks1 and Skp2 have emerged in recent years as important oncogenes and potential therapeutic targets for cancer treatment. Both genes are overexpressed in a wide spectrum of cancers and in many cases their expression levels are predictive of outcomes (reviewed in Hershko, 2008). The increased expression of Cks1 and Skp2 in tumors has been correlated with decreases in levels of the tumor suppressor, p27, suggesting that the major oncogenic activity of Cks1 and Skp2 corresponds to their role in p27 destruction. Characterization of the Drosophila Cks1 (Cks85A) and Skp2 leads the conclusion that Cks and Skp2 may have more complicated roles in the cell cycle and in cancer progression. First Drosophila Cks85A and Skp2 control growth independent of any effect on p27 levels. Second, Cks85A and Skp2 have potential tumor suppressive roles, being required for the maintenance of genomic stability (Ghorbani, 2011).
Loss of either Cks85A or Skp2 in Drosophila results in polyploidy. Flow cytometry analysis reveals up to 35% of cells being polyploid (depending on genotype and temperature). Of these, the vast majority appear to be tetraploid, with much lower frequency of cells with greater than 8n DNA content. A similar conclusion can be reached from chromosome squashes of larval brains. While tetraploidy may be more prevalent than higher ploidies, this conclusion has to be somewhat qualified. Based on the appearance of nuclei in whole mount preparations, the ploidy level of cells in wing imaginal discs seems much less than in eye imaginal discs or brains. While the degree of polyploidy in eye imaginal discs was not quantified it is noted that most nuclei posterior to the MF appear larger than those anterior to the MF, suggesting that the majority of these undifferentiated cells are polyploid. Also, the size of nuclei posterior to the MF varies considerably dependent on genotype and temperature. Presumably the larger nuclei represent cells of greater than tetraploid DNA content. Chromosome squashes on the other hand, could also lead to an underestimate of both the frequency and degree of polyploidy, as only cells that are in mitosis are examined for ploidy. If mutant cells bypass mitosis and undergo endoreplication cycles they will not be identified by this method (Ghorbani, 2011).
Cks85A and Skp2 appear to be required in multiple cell types to maintain diploidy, but interestingly the requirement for these genes is limited to late in development. In the case of Cks85A this is clearly not due to maternal rescue, as demonstrated by the finding that loss of the Maternal genes contribution of Cks85A does not result in an earlier onset or more severe polyploidy. Similar experiments could not be performed with Skp2 as the centromere-proximal location of the Skp2 gene precludes the use of mitotic recombination to generate germline clones (Ghorbani, 2011).
The distinctive phenotype observed in the eye imaginal disc also suggests a specifically late requirement for Cks85A and Skp2. During the 3rd instar, a morphogenic furrow passes from posterior to anterior of the eye imaginal disc and cells stop dividing and differentiate in its wake. Eye discs from Cks85A and Skp2 null wandering 3rd instar larvae show clear polyploidy only in mitotically active, undifferentiated cells anterior to the furrow. The simplest interpretation is that polyploidy only arises in mitotically active cells and only late in development. It is interesting that there is often a fairly abrupt transition from polyploid cells anterior to the furrow, to apparently diploid cells posterior to the furrow. Assuming that the MF is continually moving in these mutants, more of a gradient of polyploidy that extends posterior to the MF would be expected. Perhaps MF progression is also affected in these mutants, or alternatively, polyploid cells posterior to the MF are eliminated via apoptosis (Ghorbani, 2011).
In addition to polyploidy, Cks85A and Skp2losses result in a high frequency of apoptosis. Genomic instability often leads to the activation of checkpoint pathways that trigger apoptosis. Further study will be necessary to determine if apoptosis in Cks85A and Skp2 is a result of such a checkpoint dependent response to anneuploidy (Ghorbani, 2011).
A role for Skp2 and Cks85A in maintaining diploidy appears to be conserved from Drosophila to vertebrates, but how they perform this function may not be conserved. There is, in fact, little consensus on how Cks1 and Skp2 maintain diploidy in mammalian cells. Cks1 has been implicated in a redundant role with Cks2 in promoting Cdk1 and cyclin transcription and entry into mitosis (Martinsson-Ahlzen 2008). Knockdown of both Cks1 and Cks2 results in failure to enter mitosis and consequent re-replication. By a completely different mechanism, Skp2 (and by inference, Cks1) are implicated in promoting mitotic entry by regulating p27 levels (Nakayama, 2004). Neither model fits well with what is known so far about Drosophila Cks85A and Skp2. Contrary to predictions of both models, a failure to enter mitosis in is not observed Skp2 or Cks85A null cells. With respect to the latter model, p27 levels are not elevated upon Cks85A or Skp2 knockout in Drosophila. Furthermore, p27/Dap binds and inhibits only Cdk2, not the mitotic Cdk1, and it is therefore difficult to imagine how excess p27 could disrupt mitosis in Drosophila (Ghorbani, 2011).
Skp2, but as yet not Cks1, has been implicated in a completely different pathway to maintain diploidy -- preventing re-replication by promoting Cdt1 degradation. Again the current results do not support a similar model for Drosophila Skp2 (or Cks85A), as Cdt1/Dup levels are not elevated in either Skp2 or Cks85A null cells. The control of re-replication in different cell types has been found to vary greatly and to involve multiple mechanisms. It is therefore possible that Drosophila Cks85A and Skp2 are indeed involved in preventing re-replication but that they target pre-replication factors other than Cdt1 (Ghorbani, 2011).
Regardless of how Cks85A and Skp2 maintain diploidy, the current findings strongly support a model in which they function together in this capacity. Cks85A and Skp2 null mutants are similar, with Skp2 having the more severe phenotypes. This and the fact that double mutants appear similar to Skp2 alone, argue that Cks85A has little additional function outside its role with Skp2. This possibility is more dramatically illustrated by the finding that the lethality and polyploidy of Cks85A null mutants can be at least partially overcome by over-expressing Skp2. Therefore, Cks85A has little or no essential functions outside of the SCFSkp2 complex (Ghorbani, 2011).
It is also interesting that polyploidy resulting from loss of Cks85A is relatively low at 18°C but is similar to that of Skp2 null mutants at higher temperatures. This may simply reflect a need for stronger interactions between substrate and SCF complex at higher temperatures, and if so it implies that Cks85A functions essentially to increase the efficiency of SCFSkp2–substrate interactions. This may be analogous to the role of Cks2 in mitosis, where it is proposed to link its associated Cdk to specific substrates, thereby increasing the efficiency of substrate phosphorylation (Ghorbani, 2011).
While SCFSkp2 has numerous putative in vivo substrates, little is known about the role of Cks1, if any, in the recognition of these. The exception is p27, for which Cks1 appears to be absolutely essential. The results argue that in Drosophila and perhaps in other organisms, Cks85A plays a more general role in the recognition of substrates by SCFSkp2, and that the absolute requirement for mammalian Cks1 in p27 recognition may be the exception. To date there is no clear idea of what these targets are in Drosophila and it will clearly be of great interest to identify these and to determine if these are conserved (Ghorbani, 2011).
The endoreplication cycle, in which cells undergo repeated rounds of DNA replication without intervening mitosis represents a unique variation on the normal mitotic cell cycle and an excellent system for examining growth control and S-phase control in the absence of mitosis. Cks85A and Skp2 were found to be necessary for growth in endoreplicating tissues, and this requirement appears to reflect a role in the endoreplication cycle. It seems contradictory that Cks85A and Skp2 are necessary for achieving polyploidy in endoreplicating cells while they are necessary for preventing polyploidy in diploid cells. This could reflect two distinct roles for Cks85A and Skp2. Cks85A and Skp2 interact with both Cdk1 (Cdc2) and Cdk2 (Cdc2c) and it is possible that interaction with each Cdk confers different functions on the SCFSkp2 complex. In mitotic cells, Cks85A and Skp2 may interact with the mitotic Cdk1 to prevent DNA re-replication. Loss of Cdk1 and cyclin A in Drosophila results in apparent polyploidy and it is intriguing to speculate that this reflects a functional interaction with SCFSkp2. In endoreplicating tissues, where Cdk1 is not expressed (Zielke, 2008), Cks85A and Skp2 may instead interact with Cdk2 to promote S phase. It is noted Cks85A and Skp2 may be necessary for growth in mitotic tissues and this may also reflect a Cdk2-dependent role in S phase of the canonical cell cycle. Cks85A and Skp2 may therefore interact with Cdk2 to promote DNA replication in S-phase, and interact with Cdk1 following S phase to prevent DNA re-replication. It is not yet clear how Cdk association with SCFSkp2 might contribute to its function. SCFSkp2 specifically targets phospho-proteins (Nakayama, 2006) and therefore, association with Cdks may allow efficient coordination between the phosphorylation and ubiquitination of substrates. Alternatively, Cdks could play a non-catalytic, recruitment role. It has been proposed that Cdk2 helps to recruit associated p27 to the SCFSkp2 while Cdk1 may recruit cyclin A to the APC for ubiquitination (Ghorbani, 2011).
The Cks proteins are all closely related at the sequence level and share common Cdk-interacting and phospho-epitope binding domains. Cks genes from humans can replace their budding yeast counterpart, illustrating the remarkable functional conservation of these proteins. In metazoans, in which there are two Cks genes, there is also evidence of functional conservation between the two homologues: in mice, ectopic expression of Cks1 can functionally rescue meiotic defects resulting from the loss of Cks2, while mammalian Cks1 and Cks2 appear to function redundantly to promote mitosis (Martinsson-Ahlzen, 2008; Ghorbani, 2011 and references therein).
In contrast, the two Drosophila Cks proteins appear to have distinct and non-overlapping functions. Cks30A is required for spindle assembly and anaphase progression in female meiosis but has no essential function in mitotic cells. Cks30A appears to be the functional equivalent of Xenopus Xe-p9, mouse Cks2 and C. elegans Cks1 -- all of which have specific requirements in meiosis. Previous studies have shown that Cks85A cannot functionally replace Cks30A in promoting APC activity in female meiosis and this study now shows that Cks30A cannot functionally replace Cks85A in the SCFSkp2. In fact Cks30A appears to act antagonistically to Cks85A. The failure of Cks30A to functionally replace Cks85A could easily be attributed to its inability to interact with Skp2. The apparent antagonistic behavior is more difficult to explain. Cks30A promotes the APC-dependent destruction of mitotic cyclins. Therefore it is speculated that Cks30A antagonizes SCFSkp2 function through its effect on associated cyclins. Again, this model hinges on the assumption that cyclin-Cdk association with SCFSkp2 is important for its in vivo activity, a possibility that clearly needs to be tested experimentally (Ghorbani, 2011).
In conclusion this study found that the two closely related Cks genes in Drosophila are functionally distinct and that Cks85A acts mainly or perhaps exclusively as a part of the SCFSkp2 complex. SCFSkp2 has essential roles in the maintenance of diploidy and in promoting growth. It is therefore possible that the human orthologues, Cks1 and Skp2 possess both tumor promoting and tumor suppressing functions (Ghorbani, 2011).
Search PubMed for articles about Drosophila Cyclin-dependent kinase subunit 30A
Bartek, J. and Lukas, J. (2001). p27 destruction: Cks1 pulls the trigger. Nat. Cell Biol. 3: E95-E98. PubMed ID: 11283628
Bourne, Y., et al. (1996). Crystal structure and mutational analysis of the human CDK2 kinase complex with cell cycle-regulatory protein CksHs1. Cell 84: 863-874. PubMed ID: 8601310
Cullen, C. F. and Ohkura, H. (2001). Msps protein is localized to acentrosomal poles to ensure bipolarity of Drosophila meiotic spindles. Nat Cell Biol 3: 637-642. PubMed ID: 11433295
Finley, R. L. and Brent, R. (1994). Interaction mating reveals binary and ternary connections between Drosophila cell cycle regulators. Proc. Natl. Acad. Sci. 91: 12980-12984. PubMed ID: 7809159
Ganoth, D., Bornstein, G., Ko, T. K., Larsen, B., Tyers, M., Pagano, M. and Hershko, A. (2001). The cell-cycle regulatory protein Cks1 is required for SCF(Skp2)-mediated ubiquitinylation of p27. Nat. Cell Biol. 3: 321-324. PubMed ID: 11231585
Ghorbani, M., Vasavan, B., Kraja, E. and Swan, A. (2011). Cks85A and Skp2 interact to maintain diploidy and promote growth in Drosophila. Dev. Biol. 358(1): 213-23. PubMed ID: 21827746
Hadwiger, J. A., Wittenberg, C., Mendenhall, M. D. and Reed, S. I. (1989). The Saccharomyces cerevisiae CKS1 gene, a homolog of the Schizosaccharomyces pombe suc1+ gene, encodes a subunit of the Cdc28 protein kinase complex. Mol. Cell. Biol. 9: 2034-2041. PubMed ID: 2664468
Hayles, J., Beach, D., Durkacz, B. and Nurse, P. (1986). The fission yeast cell cycle control gene cdc2: isolation of a sequence suc1 that suppresses cdc2 mutant function. Mol. Gen. Genet. 202: 291-293. PubMed ID: 3010051
Hershko, D.D. (2008). Oncogenic properties and prognostic implications of the ubiquitin ligase Skp2 in cancer. Cancer 112: 1415-1424. PubMed ID: 18260093
Martinsson-Ahlzen, H. S., et al. (2008). Cyclin-dependent Kinase Associated Proteins Cks1 and Cks2 are Essential During Early Embryogenesis and for Cell Cycle Progression in Somatic Cells, Mol. Cell. Biol. 28(18): 5698-709. PubMed ID: 18625720
Morris, M. C., Kaiser, P., Rudyak, S., Baskerville, C., Watson, M. H. and Reed, S. I. (2003). Cks1-dependent proteasome recruitment and activation of CDC20 transcription in budding yeast. Nature 424: 1009-1013. PubMed ID: 12827207
Nakayama, K., et al. (2004). Skp2-mediated degradation of p27 regulates progression into mitosis. Dev. Cell. 6: 661-672. PubMed ID: 15130491
Nakayama, K. I. and Nakayama, K. (2006). Ubiquitin ligases: cell-cycle control and cancer. Nat. Rev. Cancer 6: 369-381. PubMed ID: 16633365
Ohkura, H., Garcia, M. A. and Toda, T. (2001). Dis1/TOG universal microtubule adaptors-one MAP for all? J. Cell Sci. 114: 3805-3812. PubMed ID: 11719547
Patra, D. and Dunphy, W. G. (1996). Xe-p9, a Xenopus Suc1/Cks homolog, has multiple essential roles in cell cycle control. Genes Dev. 10: 1503-1515. PubMed ID: 8666234
Patra, D. and Dunphy, W. G. (1998). Xe-p9, a Xenopus Suc1/Cks protein, is essential for the Cdc2-dependent phosphorylation of the anaphase-promoting complex at mitosis. Genes Dev. 12: 2549-2559. PubMed ID: 9716407
Patra, D., Wang, S. X., Kumagai, A. and Dunphy, W. G. (1999). The xenopus Suc1/Cks protein promotes the phosphorylation of G(2)/M regulators. J. Biol. Chem. 274: 36839-36842. PubMed ID: 10601234
Pearson, N. J., Cullen, C. F., Dzhindzhev, N. S. and Ohkura, H. (2005). A pre-anaphase role for a Cks/Suc1 in acentrosomal spindle formation of Drosophila female meiosis. EMBO Rep. 6(11): 1058-63. PubMed ID: 16170306
Pines, J. (1996). Cell cycle: reaching for a role for the Cks proteins. Curr. Biol. 6: 1399-1402. PubMed ID: 8939596
Polinko, E. S. and Strome, S. (2000). Depletion of a Cks homolog in C. elegans embryos uncovers a post-metaphase role in both meiosis and mitosis. Curr. Biol. 10: 1471-1474. PubMed ID: 11102813
Schupbach, T. and Wieschaus, E. (1989). Female sterile mutations on the second chromosome of Drosophila melanogaster. I. Maternal effect mutations. Genetics 121: 101-117. PubMed ID: 2492966
Spruck, C., Strohmaier, H., Watson, M., Smith, A. P., Ryan, A., Krek, T. W. and Reed, S. I. (2001). A CDK-independent function of mammalian Cks1: targeting of SCF(Skp2) to the CDK inhibitor p27Kip1. Mol. Cell 7: 639-650. PubMed ID: 11463388
Spruck, C. H., de Miguel, M. P., Smith, A. P., Ryan, A., Stein, P., Schultz, R. M., Lincoln, A. J., Donovan, P. J. and Reed, S. I. (2003). Requirement of Cks2 for the first metaphase/anaphase transition of mammalian meiosis. Science 300: 647-650. PubMed ID: 12714746
Swan, A., Barcelo, G. and Schupbach, T. (2005). Drosophila Cks30A interacts with Cdk1 to target Cyclin A for destruction in the female germline. Development 132: 3669-3678. PubMed ID: 16033797
Swan, A. and Schupbach, T. (2007). The Cdc20 (Fzy)/Cdh1-related protein, Cort, cooperates with Fzy in cyclin destruction and anaphase progression in meiosis I and II in Drosophila. Development 134(5): 891-9. PubMed ID: 17251266
Zielke, N., et al. (2008). The anaphase-promoting complex/cyclosome (APC/C) is required for rereplication control in endoreplication cycles, Genes Dev. 22: 1690-703. PubMed ID: 18559483
date revised: 17 January 2012 Biological Overview
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