Proliferating cell nuclear antigen


REFERENCES

Aboussekhra, A., et al. (1995). Mammalian DNA nucleotide excision repair reconstituted with purified protein components. Cell 80: 859-868.

Amador, V., Ge, S., Santamaria, P. G., Guardavaccaro, D. and Pagano, M. (2007). APC/C(Cdc20) controls the ubiquitin-mediated degradation of p21 in prometaphase. Mol. Cell 27: 462-473. PubMed Citation: 17679094

Amin, N. S. and Holm, C. (1996). In vivo analysis reveals that the interdomain region of the yeast proliferating cell nuclear antigen is important for DNA replication and DNA repair. Genetics 144(2): 479-493.

Arias, E. E. and Walter, J. C. (2006). PCNA functions as a molecular platform to trigger Cdt1 destruction and prevent re-replication. Nat. Cell Biol. 8: 84-90. Medline abstract: 16362051

Axton, J. M., et al. (1994). The inhibitor of DNA replication encoded by the Drosophila gene plutonium is a small, ankyrin repeat protein. EMBO J 13: 462-70.

Ayyagari, R., et al. (1995). A mutational analysis of the yeast proliferating cell nuclear antigen indicates distinct roles in DNA replication and DNA repair. Mol. Cell. Biol. 15: 4420-4429.

Bermudez, V. P., MacNeill, S. A., Tappin, I. and Hurwitz, J. (2000). The influence of the Cdc27 subunit on the properties of the Schizosaccharomyces pombe DNA polymerase delta. J. Biol. Chem. 277(39): 36853-62. 12124382

Besson, A., Dowdy, S. F., and Roberts, J. M. (2008). CDK inhibitors: Cell cycle regulators and beyond. Dev. Cell 14: 159-169. PubMed Citation: 18267085

Chapados, B. R., et al. (2004). Structural basis for FEN-1 substrate specificity and PCNA-mediated activation in DNA replication and repair. Cell 116: 39-50. 14718165

Chapman, D. L. and Wolgemuth, D. J. (1994). Expression of proliferating cell nuclear antigen in the mouse germ line and surrounding somatic cells suggests both proliferation-dependent and -independent modes of function. Int J Dev Biol 38: 491-497.

Chen, I. T., et al. (1995a). Characterization of p21Cip1/Waf1 peptide domains required for cyclin E/Cdk2 and PCNA interaction. Oncogene 12: 595-607

Chen, I. T., et al. (1995b). Direct interaction of Gadd45 with PCNA and evidence for competitive interaction of Gadd45 and p21Waf1/Cip1 with PCNA. Oncogene 11: 1931-1937.

Chen, J., et al. (1995). Separate domains of p21 involved in the inhibition of Cdk kinase and PCNA. Nature 374: 386-388.

Chen, J., et al. (1996). A 39 amino acid fragment of the cell cycle regulator p21 is sufficient to bind PCNA and partially inhibit DNA replication in vivo. Nucleic Acids Res. 24: 1727-1733.

Chuang, L. S., et al. (1997). Human DNA-(Cytosine-5) methyltransferase-PCNA complex as a target for p21WAF1. Science 277(5334): 1996-2000.

Claycomb, J. M., et al. (2002). Visualization of replication initiation and elongation in Drosophila, Jour. Cell Biol. 159: 225-236. 12403810

Du, W. (2000). Suppression of the rbf null mutants by a de2f1 allele that lacks transactivation domain. Development 127: 367-379.

Duronio, R. J. and O'Farrell, P. H., (1994). Developmental control of a G1-S transcriptional program in Drosophila. Development 120: 1503-1515

Ehrenhofer-Murray, A. E., Kamakaka, R. K. and Rine, J. (1999). A Role for the replication proteins PCNA, RF-C, Polymerase episilon and Cdc45 in transcriptional silencing in Saccharomyces cerevisiae. Genetics 153: 1171-1182.

Fan, Z., et al. (1995). Prolonged induction of p21Cip1/WAF1/CDK2/PCNA complex by epidermal growth factor receptor activation mediates ligand-induced A431 cell growth inhibition. J. Cell Biol. 131: 235-242.

Flores-Rozas, H., et al. (1994). Cdk-interacting protein 1 directly binds with proliferating cell nuclear antigen and inhibits DNA replication catalyzed by the DNA polymerase delta holoenzyme. Proc. Natl. Acad. Sci. 91(18): 8655-8659.

Fotedar, R., et al. (1996). A conserved domain of the large subunits of replication factor C binds PCNA and acts like a dominant negative inhibitor of DNA replication in mammalian cells. EMBO J. 15: 4423-33.

Frank, G., Qiu, J., Zheng, L., and Shen, B. (2001). Stimulation of eukaryotic flap endonuclease-1 activities by proliferating cell nuclear antigen (PCNA) is independent of its in vitro interaction via a consensus PCNA binding region. J. Biol. Chem. 276: 36295-36302. 11477073

Fukuda, K., et al. (1995). Structure-function relationship of the eukaryotic DNA replication factor, proliferating cell nuclear antigen. J. Biol. Chem. 270(38): 22527-22534.

Furuya, K., et al. (2004). Chk1 activation requires Rad9 S/TQ-site phosphorylation to promote association with C-terminal BRCT domains of Rad4TOPBP1. Genes Dev. 18: 1154-1164. 1515558

Fuss, B., et al. (2001). Control of endoreduplication domains in the Drosophila gut by the knirps and knirps-related genes. Mech. Dev. 100: 15-23. 11118880

Gary, R., et al. (1997). The DNA repair endonuclease XPG binds to proliferating cell nuclear antigen (PCNA) and shares sequence elements with the PCNA-binding regions of FEN-1 and cyclin-dependent kinase inhibitor p21. J. Biol. Chem. 272(39): 24522-24529.

Georlette, D., et al. (2007). Genomic profiling and expression studies reveal both positive and negative activities for the Drosophila Myb MuvB/dREAM complex in proliferating cells, Genes Dev. 21: 2880–2896. PubMed Citation: 17978103

Gomes, X. V. and Burgers, P. M. (2000). Two modes of FEN1 binding to PCNA regulated by DNA. EMBO J. 19: 3811-3821. 10899134

Gulbis, J. M., et al. (1996). Structure of the C-terminal region of p21(WAF1/CIP1) complexed with human PCNA. Cell 87(2): 297-306.

Hayashi, Y., et al. (1997). Identification of CFDD (common regulatory factor for DNA replication and DREF genes) and role of its binding site in regulation of the proliferating cell nuclear antigen gene promoter. J. Biol. Chem. 272(36): 22848-22858.

Henderson, D. S., et al. (1994). Mutagen sensitivity and suppression of position-effect variegation result from mutations in mus209, the Drosophila gene encoding PCNA. EMBO J 13: 1450-9.

Henderson, D. S., et al. (2000). Mutual correction of faulty PCNA subunits in temperature-sensitive lethal mus209 mutants of Drosophila melanogaster Genetics 154: 1721-1733

Hirose, F., Yamaguchi, M. and Matsukage, A. (1994). Repression of regulatory factor for Drosophila DNA replication-related gene promoters by zerknullt homeodomain protein. J. Biol. Chem. 269: 2937-42.

Hirose, F., et al. (1996). Isolation and characterization of cDNA for DREF, a promoter-activating factor for Drosophila DNA replication-related genes. J. Biol. Chem. 271: 3930-3937

Hochheimer, A., et al. (2002). TRF2 associates with DREF and directs promoter-selective gene expression in Drosophila. Nature 420(6914): 439-45. 12459787

Hosfield, D. J., et al. (1998). Structure of the DNA repair and replication endonuclease and exonuclease FEN-1: coupling DNA and PCNA binding to FEN-1 activity. Cell 95(1): 135-46.

Huang, D. Y. and Prystowsky, M. B. (1996). Identification of an essential cis-element near the transcription start site for transcriptional activation of the proliferating cell nuclear antigen gene. J. Biol. Chem. 271(2): 1218-1225.

Huang, F., Saraf, A., Florens, L., Kusch, T., Swanson, S. K., Szerszen, L. T., Li, G., Dutta, A., Washburn, M. P., Abmayr, S. M. and Workman, J. L. (2016). The Enok acetyltransferase complex interacts with Elg1 and negatively regulates PCNA unloading to promote the G1/S transition. Genes Dev 30: 1198-1210. PubMed ID: 27198229

Hayashi, Y., et al. (1999). A binding site for the transcription factor Grainyhead/Nuclear transcription factor-1 contributes to regulation of the Drosophila Proliferating cell nuclear antigen gene promoter. J. Biol. Chem. 274: 49: 35080-35088

Jin, Y., Zeng, S. X., Sun, X. X., Lee, H., Blattner, C., Xiao, Z. and Lu, H. (2008). MDMX promotes proteasomal turnover of p21 at G1 and early S phases independently of, but in cooperation with, MDM2. Mol. Cell. Biol. 28: 1218-1229. PubMed Citation: 18086887

Jonsson, Z. O., Hindges, R., and Hubscher, U. (1998). Regulation of DNA replication and repair proteins through interaction with the front side of proliferating cell nuclear antigen. EMBO J. 17: 2412-2425. 9545252

Kanellis, P., Agyei, R. and Durocher, D. (2003). Elg1 forms an alternative PCNA-interacting RFC complex required to maintain genome stability. Curr. Biol. 13: 1583-1595. 13678589

Kang, M. J., et al. (1997). Cytoplasmic localization of cyclin D3 in seminiferous tubules during testicular development. Exp. Cell Res. 234(1): 27-36.

Kelman, Z., and O'Donnell, M. (1995). Structural and functional similarities of prokaryotic and eukaryotic DNA polymerase sliding clamps. Nucleic Acids Res. 23(18): 3613-3620.

Kim, Y., Starostina, N. G. and Kipreos, E. T. (2008). The CRL4Cdt2 ubiquitin ligase targets the degradation of p21Cip1 to control replication licensing. Genes Dev. 22(18): 2507-19. PubMed Citation: 18794348

Klungland, A. and Lindahl, T. (1997). Second pathway for completion of human DNA base excision-repair: reconstitution with purified proteins and requirement for DNase IV (FEN1). EMBO J. 16(11): 3341-3348.

Korenjak, M. et al. (2004). Native E2F/RBF complexes contain Myb-interacting proteins and repress transcription of developmentally controlled E2F target genes, Cell 119: 181–193. PubMed Citation: 15479636

Levin, D. S., et al. (1997). An interaction between DNA ligase I and proliferating cell nuclear antigen: implications for Okazaki fragment synthesis and joining. Proc. Natl. Acad. Sci. 94(24): 12863-8.

Levin, D. S., et al. (2000). Interaction between PCNA and DNA ligase I is critical for joining of Okazaki fragments and long-patch base-excision repair. Curr. Biol. 10: 919-922. 10959839

Lewis, P. W., et al. (2004). Identification of a Drosophila Myb-E2F2/RBF transcriptional repressor complex. Genes Dev. 18: 2929-2940. PubMed Citation: 15545624

Li, J., et al. (2008). Synergistic function of E2F7 and E2F8 is essential for cell survival and embryonic development, Dev. Cell 14: 62–75. PubMed Citation: 18194653

Li, R., et al. (1996). Subcellular distribution of p21 and PCNA in normal and repair-deficient cells following DNA damage. Curr. Biol. 6: 189-199.

Li, X., Li, J., Harrington, J., Lieber, M. R., and Burgers, P. M. (1995). Lagging strand DNA synthesis at the eukaryotic replication fork involves binding and stimulation of FEN-1 by proliferating cell nuclear antigen. J. Biol. Chem. 270: 22109-22112. 7673186

Liu, J. and Schwartz, J. H. (2003). The cytoplasmic polyadenylation element binding protein and polyadenylation of messenger RNA in Aplysia neurons. Brain Res. 959: 68-76. 12480159

Luo, Y., Hurwitz, J. and Massague, J. (1995). Cell-cycle inhibition by independent CDK and PCNA binding domains in p21Cip1. Nature 375: 159-161. <

Maga, G., et al. (1997). Phosphorylation of the PCNA binding domain of the large subunit of replication factor C by Ca2+/calmodulin-dependent protein kinase II inhibits DNA synthesis. Biochemistry 36(18): 5300-5310.

Matsukage, A., et al. (1995). The DRE sequence TATCGATA, a putative promoter-activating element for Drosophila melanogaster cell-proliferation-related genes. Gene 166: 233-236.

Matsumiya, S., Ishino, S., Ishino, Y., and Morikawa, K. (2002). Physical interaction between proliferating cell nuclear antigen and replication factor C from Pyrococcus furiosus. Genes Cells 7: 911-922. 12296822

Matsumoto, Y., Kim, K. and Bogenhagen, D. F. (1994). Proliferating cell nuclear antigen-dependent abasic site repair in Xenopus laevis oocytes: an alternative pathway of base excision DNA repair. Mol Cell Biol 14: 6187-6197.

Montecucco, A., et al. (1998). DNA ligase I is recruited to sites of DNA replication by an interaction with proliferating cell nuclear antigen: identification of a common targeting mechanism for the assembly of replication factories. EMBO J. 17(13): 3786-95.

Morris, G. F., Bischoff, J. R. and Mathews, M. B. (1996). Transcriptional activation of the human proliferating-cell nuclear antigen promoter by p53. Proc. Natl. Acad. Sci. 93: 895-899.

Mossi, R., et al. (1997). Replication factor C interacts with the C-terminal side of proliferating cell nuclear antigen. J. Biol. Chem. 272(3): 1769-1776.

Mozzherin, D. J., et al. (1996). Proliferating cell nuclear antigen promotes misincorporation catalyzed by calf thymus DNA polymerase delta. J. Biol. Chem. 271(49): 31711-31717.

Nakanishi, M., et al. (1995). The C-terminal region of p21SDI1/WAF1/CIP1 is involved in proliferating cell nuclear antigen binding but does not appear to be required for growth inhibition. J Biol Chem 270: 17060-17063.

Ng, L., et al. (1990). Drosophila proliferating cell nuclear antigen. Structural and functional homology with its mammalian counterpart. J Biol Chem 265: 11948-54.

Ng, L., et al. (1997). Interaction of DNA polymerase delta, proliferating cell nuclear antigen, and synthetic oligonucleotide template-primers. Analysis by polyacrylamide gel electrophoresis-band mobility shift assay. J. Biol. Chem. 268: 13571-6.

Nishitani, N., et al. (2006). Two E3 ubiquitin ligases, SCF-Skp2 and DDB1-Cul4, target human Cdt1 for proteolysis, EMBO J. 25: 1126–1136. PubMed Citation: 16482215

Ohno, K., et al. (1996). Transcriptional regulation of the Drosophila CycA gene by the DNA replication-related element (DRE) and DRE binding factor (DREF). Nucleic Acids Res. 24(20): 3942-3946.

Pagano, M., et al. (1994). Cyclin D1-mediated inhibition of repair and replicative DNA synthesis in human fibroblasts. Genes Dev. 8: 1627-39.

Podust, V. N., et al. (1995). Mechanism of inhibition of proliferating cell nuclear antigen-dependent DNA synthesis by the cyclin-dependent kinase inhibitor p21. Biochemistry 34: 8869-8875.

Frolov, M. V., et al. (2001). Functional antagonism between E2F family members Genes Dev. 15: 2146-2160. 11511545

Ressler, S., Morris, G. F. and Marriott, S. J. (1997). Human T-cell leukemia virus type 1 Tax transactivates the human proliferating cell nuclear antigen promoter. J. Virol. 71(2): 1181-1190.

Reynolds, N., et al. (2000). Essential interaction between the fission yeast DNA polymerase delta subunit Cdc27 and Pcn1 (PCNA) mediated through a C-terminal p21(Cip1)-like PCNA binding motif. EMBO J. 19(5): 1108-18. 10698951

Rossi, R., et al. (1999). The replication factory targeting sequence/PCNA-binding site is required in G1 to control the phosphorylation status of DNA ligase I. EMBO J. 18: 5745-5754.

Royzman, I., Whittaker, A. J. and Orr-Weaver, T. L. (1997). Mutations in Drosophila DP and E2F distinguish G1-S progression from an associated transcriptional program. Genes & Dev. 11: 1999-2011.

Royzman, I., et al. (2002). The E2F cell cycle regulator is required for Drosophila nurse cell DNA replication and apoptosis. Mech. Dev. 119: 225-237. 12464435

Ryu, J. R., et al. (1997). Transcriptional regulation of the Drosophila-raf proto-oncogene by the DNA replication-related element (DRE)/DRE-binding factor (DREF) system. Nucleic Acids Res. 25(4): 794-799.

Sakurai, S., et al. (2005). Structural basis for recruitment of human flap endonuclease 1 to PCNA. EMBO J. 24: 683-693. 15616578

Savio, M., et al. (1996). p21waf1/cip1 protein associates with the detergent-insoluble form of PCNA concomitantly with disassembly of PCNA at nucleotide excision repair sites. Oncogene 13(8): 1591-1598.

Senga, T., Sivaprasad, U., Zhu, W., Park, J. H., Arias, E. E., Walter, J. C. and Dutta, A. (2006). PCNA is a cofactor for Cdt1 degradation by CUL4/DDB1-mediated N-terminal ubiquitination. J. Biol. Chem. 281: 6246-6252. Medline abstract: 16407252

Seto, H., Hayashi, Y., Kwon, E., Taguchi, O. and Yamaguchi, M. (2006). Antagonistic regulation of the Drosophila PCNA gene promoter by DREF and Cut. Genes Cells. 11(5): 499-512. 16629902

Sharp, J. A., et al. (2001). Yeast histone deposition protein Asf1p requires Hir proteins and PCNA for heterochromatic silencing. Curr. Biol. 11: 463-473

Shibahara, K. and Stillman, B. (1999). Replication-dependent marking of DNA by PCNA facilitates CAF-1-coupled inheritance of chromatin. Cell 96(4): 575-85.

Shibutani, S. T., et al. (2008). Intrinsic negative cell cycle regulation provided by PIP box- and Cul4Cdt2-mediated destruction of E2f1 during S phase. Dev. Cell 15(6): 890-900. PubMed Citation: 19081076

Shikata, K., et al. (2001). The human homolog of fission Yeast cdc27, p66, is a component of active human DNA polymerase delta. J. Biochem. (Tokyo). 129(5): 699-708. 11328591

Shivakumar, C. V., et al. (1995). Wild-type human p53 transactivates the human proliferating cell nuclear antigen promoter. Mol Cell Biol 15: 6785-6793.

Smith, M. L., et al. (1994). Interaction of the p53-regulated protein Gadd45 with proliferating cell nuclear antigen. Science 266: 1376-1380.

Stevaux, O., et al. (2002). Distinct mechanisms of E2F regulation by Drosophila RBF1 and RBF2. EMBO J. 21: 4927-4937. 12234932

Stevaux, O., et al. (2005). Retinoblastoma family 2 is required in vivo for the tissue-specific repression of dE2F2 target genes. Cell Cycle 4: 1272–1280. PubMed Citation: 16082225

Sundqvist, A., Sollerbrant, K. and Svensson, C. (1998). The carboxy-terminal region of adenovirus E1A activates transcription through targeting of a C-terminal binding protein-histone deacetylase complex. FEBS Lett. 429(2): 183-8.

Thacker, S. A., Bonnette, P. C. and Duronio, R. J. (2003). The contribution of E2F-regulated transcription to Drosophila PCNA gene function. Curr. Biol. 13: 53-58. 12526745

Tom, S., Henricksen, L. A., and Bambara, R. A. (2000). Mechanism whereby proliferating cell nuclear antigen stimulates flap endonuclease 1. J. Biol. Chem. 275: 10498-10505. 10744741

Torres-Ramos, C. A., et al. (1996). Requirement of proliferating cell nuclear antigen in RAD6-dependent postreplicational DNA repair. Proc. Natl. Acad. Sci. 93(18): 9676-9681.

Torres-Ramos, C. A., Prakash, S. and Prakash, L. (1997). Requirement of yeast DNA polymerase delta in post-replicational repair of UV-damaged DNA. J. Biol. Chem. 272(41): 25445-25448.

Uhlmann, F., et al. (1997a). Deletion analysis of the large subunit p140 in human replication factor C reveals regions required for complex formation and replication activities. J. Biol. Chem. 272(15): 10058-10064.

Uhlmann, F., et al. (1997b). Identification of regions within the four small subunits of human replication factor C required for complex formation and DNA replication. J. Biol. Chem. 272(15): 10065-10071.

Umar, A., et al. (1996). Requirement for PCNA in DNA mismatch repair at a step preceding DNA resynthesis. Cell 87(1): 65-73.

Waga, S., et al. (1994). The p21 inhibitor of cyclin-dependent kinases controls DNA replication by interaction with PCNA. Nature 369: 574-8.

Waga, S. and Stillman, B. (1998). Cyclin-dependent kinase inhibitor p21 modulates the DNA primer-template recognition complex. Mol. Cell. Biol. 18(7): 4177-4187.

Warbrick, E., et al. (1997). Homologous regions of Fen1 and p21Cip1 compete for binding to the same site on PCNA: a potential mechanism to co-ordinate DNA replication and repair. Oncogene 14(19): 2313-2321.

Watanabe, H., et al. (1998). Suppression of cell transformation by the cyclin-dependent kinase inhibitor p57(KIP2 )requires binding to proliferating cell nuclear antigen. Proc. Natl. Acad. Sci. 95(4): 1392-1397.

Wu, X., et al. (1996). Processing of branched DNA intermediates by a complex of human FEN-1 and PCNA. Nucleic Acids Res. 24: 2036-2043.

Yamaguchi, M., et al. (1990). Drosophila proliferating cell nuclear antigen (cyclin) gene: structure, expression during development, and specific binding of homeodomain proteins to its 5'-flanking region. Mol. Cell. Biol. 10: 872-9.

Yamaguchi, M., Date, T. and Matsukage, A. (1991a). Distribution of PCNA in Drosophila embryo during nuclear division cycles. J. Cell Sci. 100 ( Pt 4): 729-33.

Yamaguchi, M., et al. (1991b). Repression of the Drosophila proliferating-cell nuclear antigen gene promoter by Zerknullt protein. Mol. Cell. Biol. 11: 4909-17

Yamaguchi, M., Nishida, Y. and Matsukage, A. (1995a). Role of homeodomain protein binding region in the expression of Drosophila proliferating cell nuclear antigen gene: analysis with transgenic flies. Gene Expr. 4: 183-193

Yamaguchi, M., et al., (1995b). A nucleotide sequence essential for the function of DRE, a common promoter element for Drosophila DNA replication-related genes. J. Biol. Chem. 270: 15808-15814. 7797583

Yamaguchi, M., et al. (1995c). Distribution of PCNA during postblastoderm cell division cycles in the Drosophila melanogaster embryo: effect of a string- mutation. Cell Struct. Funct. 20: 47-57

Yamaguchi, M., Hayashi, Y. and Matsukage, A. (1995d). Essential role of E2F recognition sites in regulation of the proliferating cell nuclear antigen gene promoter during Drosophila development. J. Biol. Chem. 270: 25159-25165.

Yamaguchi, M., Hirose, F. and Matsukage, A. (1996). Roles of multiple promoter elements of the proliferating cell nuclear antigen gene during Drosophila development. Genes Cells 1(1) :47-58.

Yamamoto, Y., et al. (1997). The cramped gene of Drosophila is a member of the Polycomb-group, and interacts with mus209, the gene encoding Proliferating Cell Nuclear Antigen. Development 124(17): 3385-3394.

Yang, X. H. Shiotani, B., Classon, M. and Zou, L. (2008). Chk1 and Claspin potentiate PCNA ubiquitination. Genes Dev. 22: 1147-1152. PubMed Citation: 18451105

Yao, N., et al. (1996). Clamp loading, unloading and intrinsic stability of the PCNA, beta and gp45 sliding clamps of human, E. coli and T4 replicases. Genes Cells 1(1): 101-113.

Yeeles, J. T., Janska, A., Early, A. and Diffley, J. F. (2017). How the eukaryotic replisome achieves rapid and efficient DNA replication. Mol Cell 65: 105-116. PubMed ID: 27989442

Zhang, G., et al. (1999). Studies on the interactions between human replication factor C and human proliferating cell nuclear antigen. Proc. Natl. Acad. Sci. 96(5): 1869-1874.

Zhang, Z., Shibahara, K. and Stillman, B. (2000). PCNA connects DNA replication to epigenetic inheritance in yeast. Nature 408(6809): 221-5

Zhou, J. Q., et al. (1997). The small subunit is required for functional interaction of DNA polymerase delta with the proliferating cell nuclear antigen. Nucleic Acids Res. 25(6): 1094-1099.


Proliferating cell nuclear antigen: Biological Overview | Evolutionary Homologs | Regulation | Developmental Biology | Effects of Mutation

date revised: 17 December 2021

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