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  Ionophore Initiated Calcium Waves
To see the wave of release of calcium ions in this transparent fish egg, it was injected with aequorin, a protein that emits light when it binds to calcium ions. This unfertilized egg then gets exposed to an ionophore that makes the egg permeable to calcium ions in the medium. Waves of calcium ion release occur at multiple sites in this unfertilized egg. This sequence is approximately twice as fast as it actually occurs.
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