Our group is interested in understanding how primary cilia regulate the proliferation of adult neural stem cells. For this we have developed protocols for the isolation of neural stem cells and relative cilia from the embryonic and the adult brain. Further information relative to the approach used for the isolation of neural stem cells can be found in Khatri et al., Sci. Rep 2014.
The project aims at understanding the molecular programs associated to the regulation of signal transduction in neural stem cells by identifying the protein components of the cilia responsible for signal transduction.
The project will involve purification of primary cilia, protein solubilization and identification of the ciliary proteome by antibody array and mass spectrometry.
The lab is located within the Interdisciplinary Center for Neurosciences in Heidelberg and offers excellent research facilities in an intellectually stimulating environment.
A background in proteomics, flow cytometry and or cilia analysis would be considered an advantage.
Application should be sent to:
Dr. Francesca Ciccolini email@example.com